Antioxidant Effect of Alpha-Linolenic Acid during In Vitro Maturation in Porcine Oocytes

Lee, Jieun; Hwangbo, Yong; Kim, Hwa-Young; Cheong, Hyeonsook; Yang, Boo‐Keun; Park, Choon‐Keun

doi:10.12749/rdb.2017.41.4.65

Cited by 2 publications

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“…As a marker of cellular maturation, GSH is increased in oocytes during maturation (DeMatos et al, 2000) and is involved with male pronuclei formation in oocytes (Mori et al, 2000). In our previous study, reduced GSH level in oocytes by H 2 O 2 was recovered in 50 μM ALA and it suggested that ALA treatment could promote GSH synthesis in oocytes (Lee et al, 2017b). Furthermore, FAs are regarded as potential metabolic substrates in oocytes and early-stage embryos and are required as an energy source for the metabolism of embryos in pre-implantation of cows and pigs (Sturmey et al, 2009).…”

Section: Discussionmentioning

confidence: 84%

Alpha-Linolenic Acid: It Contribute Regulation of Fertilization Capacity and Subsequent Development by Promoting of Cumulus Expansion during Maturation

Lee¹,

Hwangbo²,

Cheong³

et al. 2018

Dev. Reprod.

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The objective of this study was to evaluate the effects of alpha-linolenic acid (ALA) during in vitro maturation (IVM) on cumulus expansion, nuclear maturation, fertilization capacity and subsequent development in porcine oocytes. The oocytes were incubated with 0, 25, 50, and 100 μM ALA. Cumulus expansion was measured at 22 h, and gene expresison and nuclear maturation were analyzed at 44 h after maturation. Then, mature oocytes with ALA were inseminated, and fertilization parameters and embryo development were evaluated. In results, both of cumulus expansion and nuclear maturation were increased in 50 μM ALA groups compared to control groups (p<0.05). However, expression of gap junction protein alpha 1 (GJA1, cumulus expansion-related gene), delta-6 desaturase (FADS1, fatty acid metabolism-related gene), and delta-5 desaturase (FADS2) mRNA in cumulus cells were reduced by 50 μM ALA treatment (p<0.05). Cleavage rate was enhanced in 25 and 50 μM ALA groups (p<0.05), especially, treatment of 50 μM ALA promoted early embryo develop to 4 and 8 cell stages (p<0.05). However, blastocyst formation and number of cells in blastocyst were not differ in 25 and 50 μM ALA groups. Our findings show that ALA treatment during maturation could improve nuclear maturation, fertilization, and early embryo development through enhancing of cumulus expansion, however, fatty acid metabolism- and cumulus expansion-related genes were down-regulated. Therefore, addition of ALA during IVM of oocytes could improve fertilization and developmental competence, and further studies regarding with the mechanism of ALA metabolism are needed.

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