Antioxidant effect of high-density lipoproteins in peroxidation of low-density lipoproteins

Klimov, A. N.; Kozhemyakin, L. A.; Pleskov, V M; Andreeva, L. I.

doi:10.1007/bf00841817

Cited by 21 publications

(10 citation statements)

References 9 publications

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“…Oxidation of LDL is thought to be a key element in the atherosclerotic process (42). HDL can inhibit the Cu2+-catalyzed oxidation of LDL in vitro (37,38). We have found that Lp(A-I) are more effective in the inhibition of LDL oxidation than ultracentrifugally isolated HDL and that a significant part of this in vitro inhibition can be attributed to the quantitatively minor subfractions of transferrin-and ceruloplasmin-containing Lp(A-I).…”

Section: Discussionmentioning

confidence: 91%

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Binding of transition metals by apolipoprotein A-I-containing plasma lipoproteins: inhibition of oxidation of low density lipoproteins.

Kunitake

Jarvis

Hamilton

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

125

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We have found transition metals tightly bound to apolipoprotein A-I-containing lipoproteins [Lp(A-I)] isolated by selected affinity immunosorption from human serum. Prominent among the metal ions detected were iron and copper. By immunoblotting the proteins of Lp(A-I), we detected both transferrin and ceruloplasmin. The transferrincontaining Lp(A-I) particles, isolated by selected aflmity immunosorption against transferrin, were larger (mean diameter of 14.2 nm) and had a higher protein content than most high density lipoproteins (HDL). Ultracentrifugally isolated HDL were found to contain much less transferrin, whereas transferrin was found associated with apolipoprotein A-I from the >1.21-g/ml ultracentrifugal fraction. This suggests that the complex is not recovered in the classic HDL density interval because of its very high density. HDL inhibit copper-catalyzed oxidation of low density lipoproteins (LDL) in vitro. We have found that immunoisolated Lp(A-I) are an order of magnitude more effective in inhibiting the oxidation of LDL than ultracentrifugally isolated HDL, on the basis of protein mass. When

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Section: Discussionmentioning

confidence: 91%

“…HDL can inhibit Cu2+-catalyzed oxidation of LDL in vitro (37,38). Immunoisolated Lp(A-I) were almost an order of magnitude more effective at inhibiting the production of TBARS in LDL than an equal mass of ultracentrifugally isolated HDL from the same individual (Fig.…”

Section: Methodsmentioning

confidence: 95%

Binding of transition metals by apolipoprotein A-I-containing plasma lipoproteins: inhibition of oxidation of low density lipoproteins.

Kunitake

Jarvis

Hamilton

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

125

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“…High density lipoproteins do not share these properties and experiments suggest that they may even protect low density lipoproteins from peroxidation. 30 This constellation of effects-namely, endothelial cell injury, uncontrolled lipid uptake by macrophages, reduced endothelial prostacyclin synthesis, and associated thrombogenicity-have all been strongly implicated in the pathogenesis of atherosclerosis. '6"' The potential role of lipid peroxides in atherogenesis is further supported by the increase in serum and aortic lipid peroxide concentrations found in animals maintained with atherogenic diets'2 2 and epidemiological data showing increased serum lipoperoxide concentrations in the children of parents suffering premature myocardial infarction.33 Further work is in progress to see whether plasma lipid peroxide values might provide a useful index of the severity ofatherosclerosis.…”

Section: Statisticsmentioning

confidence: 99%

Lipid peroxides and atherosclerosis.

Stringer

Görög

Freeman

et al. 1989

BMJ

328

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Plasma lipid peroxide concentrations were measured in 100 patients with occlusive arterial disease proved angiographically (50 patients with ischaemic heart disease, 50 with peripheral arterial disease) and compared with values in 75 control patients with no clinical evidence of atherosclerosis. Lipid peroxide concentrations were significantly higher in patients both with ischaemic heart disease (median 4-37 iimol/l (interquartile range 3-85-5-75 [tmol/l); p<0-001) and with peripheral arterial disease (median 4-37 [smoltl (3-88-5-21 [mol/l); p<0001) than in controls (median 3-65 [tmol/l (interquartile range 3-29-3-89 [mol/l)).Overall there was a significant but weak correlation between plasma lipid peroxide and plasma triglyceride concentrations (r,=0-25; p<0-001) but not between plasma lipid peroxide and plasma total cholesterol concentrations. Furthermore, hypertension, obesity, diabetes, smoking, positive family history, and intake of fi blockers and thiazide diuretics were not associated with significant differences in lipid peroxide values.This study provides clinical support to experimental data indicating that peroxidised lipids may be important in atherogenesis and its complications and also suggests that peroxidised lipids may provide an index of the severity of atherosclerosis.Thrombosis Research

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“…Injection of HDL 3 into hypercholesterolemic rabbits reduced the levels of conjugated dienes in plasma. 32 The levels of HDL cholesterol significantly correlated with the levels of conjugated dienes in the plasma of healthy subjects and patients with coronary artery disease. 33 The results of the present study demonstrated that HDL attenuates the formation of conjugated dienes in LDL after incubation with ECs.…”

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confidence: 99%

Impact of Antioxidants and HDL on Glycated LDL–Induced Generation of Fibrinolytic Regulators From Vascular Endothelial Cells

Ren

Shen

2000

ATVB

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Abstract-Hyperglycemia and dyslipoproteinemia are biochemical markers of diabetes mellitus (DM). Elevated levels of plasminogen activator inhibitor-1 (PAI-1) with and without reduction of tissue plasminogen activator (tPA) in plasma have been frequently found in patients with DM. Our previous studies indicated that glycation enhances low density lipoprotein (LDL)-induced production of PAI-1 and further decreases tPA generation in vascular endothelial cells (ECs). The present study demonstrated that treatment with antioxidants, butylated hydroxytoluene or vitamin E, blocked native LDL-and glycated LDL-induced changes in PAI-1 and tPA generation in ECs. Native or glycated high density lipoprotein (HDL) did not significantly alter tPA generation in ECs. Glycated but not native HDL (Ն100 g/mL) moderately increased PAI-1 release from ECs. Cotreatment with native or glycated HDL inhibited LDL-induced or glycated LDL-induced changes in PAI-1 and tPA generation in ECs. The abundance of conjugated dienes was increased in glycated or EC-modified LDL. Treatment with butylated hydroxytoluene, vitamin E, or HDL reduced the abundance of conjugated dienes in glycated or EC-modified LDL. The effects of antioxidants and HDL on LDL-induced or its glycated LDL-induced changes in the generation of PAI-1 and tPA were also found in cultured human coronary artery ECs. The findings of the present study suggest that antioxidants and HDL may attenuate native LDL-or glycated LDL-induced changes in the generation of fibrinolytic regulators from vascular ECs, which possibly results from their inhibition on the lipid peroxidation of LDL particles. Treatment with antioxidants or hypolipidemic agents potentially improves fibrinolytic activity and reduces thrombotic tendencies in patients with DM. Key Words: LDL Ⅲ glycation Ⅲ antioxidants Ⅲ HDL Ⅲ plasminogen activator inhibitor-1 H yperglycemia and dyslipoproteinemia, 2 major biochemical markers in diabetes mellitus (DM), have been implicated in the development of cardiovascular complications in patients with DM. Intravascular thrombosis plays a critical role in the development of ischemic events with and without the presence of atherosclerotic lesions. The formation of thrombus in the vasculature mainly depends on the balance between coagulation and fibrinolysis. The release of fibrinolytic activity from the vascular wall responding to temporary occlusion was impaired in patients with DM. 1,2 The production of plasminogen activator inhibitor-1 (PAI-1) and tissue plasminogen activator (tPA) in vascular endothelial cells (ECs) is modulated by thrombin, 3 glucose, 4 proinsulin, 5 growth factors, 6 and several types of plasma lipoproteins. [7][8][9][10][11][12][13] Elevated levels of glycated LDL were detected in the plasma of patients with DM. 14,15 Previous studies by our group demonstrated that glycation enhanced LDL-induced overproduction of PAI-1 and further reduced the generation of tPA from cultured vascular ECs. 13 Glycation increases the oxidative stress of lipoproteins. 16 Treatment with...

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Antioxidant effect of high-density lipoproteins in peroxidation of low-density lipoproteins

Cited by 21 publications

References 9 publications

Binding of transition metals by apolipoprotein A-I-containing plasma lipoproteins: inhibition of oxidation of low density lipoproteins.

Binding of transition metals by apolipoprotein A-I-containing plasma lipoproteins: inhibition of oxidation of low density lipoproteins.

Lipid peroxides and atherosclerosis.

Impact of Antioxidants and HDL on Glycated LDL–Induced Generation of Fibrinolytic Regulators From Vascular Endothelial Cells

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