Antioxidant Effect of Sericin in Brain and Peripheral Tissues of Oxidative Stress Induced Hypercholesterolemic Rats

Abstract

This study evaluated the antioxidant effect of crude sericin extract (CSE) from Antheraea assamensis in high cholesterol fed rats. Investigation was conducted by administering graded oral dose of 0.25 and 0.5 gm/kg body weight (b.w.)/day of CSE for a period of 28 days. Experiments were conducted in 30 rats and were divided into five groups: normal control, high cholesterol fed (HCF), HCF + 0.065 gm/kg b.w./day fenofibrate (FF), HCF + sericin 0.25 gm/kg b.w./day (LSD), and HCF + sericin 0.5 gm/kg b.w./day (HSD). In brain, heart, liver, serum, and kidney homogenates nitric oxide (NO), thiobarbituric acid reactive substances (TBARS), protein carbonyl content (PCC), superoxide dismutase, reduced glutathione (GSH) was measured. LSD treatment prevented the alterations in GSH and PCC levels in hypercholesterolemic (HyC) brain tissue homogenates of rats. CSE lowers the serum total cholesterol level in HyC rats by promoting fecal cholesterol (FC) excretion. CSE increases FC level by promoting inhibition of cholesterol absorption in intestine. The endogenous antioxidant reduced significantly and the oxidative stress marker TBARS level increases significantly in the peripheral tissue of HCF rats. However, the administration of LSD and HSD exhibited a good antioxidant activity by reducing the TBARS level and increasing the endogenous antioxidant in peripheral tissue. In addition, a histological examination revealed loss of normal liver and kidney architecture in cholesterol fed rats which were retained in sericin treated groups. The findings of this study suggested that CSE improves hypercholesterolemia in rats fed a HyC diet. Clinical relevance of this effect of CSE seems worthy of further studies.

The Potential of Moringa oleifera to Induce Cerebral Leptin mRNA Expression and to Attenuate Oxidative Stress, Cognitive and Motor Deficits, Depression- and Anxiety- Like Behavior in Experimental Obese Model

“…Rats fed the HCD for a long period, would exhibit higher production of ROS in their brain tissues rather than in the controls. Therefore, the brain vulnerability to oxidative stress is associated with GSH depletion and lipid peroxidation rise 51 . Brain dysfunction is considered a potential results of obesity as a stressful condition implicated in induction of ROS and oxidative stress 56 .…”

Phytochemical screening, antioxidant, antityrosinase, and antigenotoxic potential of Amaranthus viridis extract

“…Total content of polyphenols, flavonoids, and tannin in the extracts was determined as previously reported. [ 14 15 16 ] The amount of total polyphenol was calculated as a catechin equivalent from the calibration curve of catechin as a reference standard and expressed as mg catechin/g dry weight of the sample. Flavonoid concentration was expressed as quercetin equivalent, i.e., expressed as mg quercetin/g dry weight of the sample.…”

Extraction and antioxidant activity of sericin, a protein from silk

“…J. Food Technol., Campinas, v. 23, e2019058, 2020 | https://doi.org/10.1590/1981-6723.05819 7/14 2016; Napavichayanun et al, 2017;Deori et al, 2016;Sangwong et al, 2016;Kumar & Mandal (2017); Fan et al, 2010;Kaewkon et al, 2012;Zhaorigetu et al, 2001;Dash et al, 2008), and inhibits tyrosinase activity (Aramwit et al, 2010a;Kato et al, 1998;Thongsook & Tiyaboonchai, 2011;Puangphet et al, 2015;Wu et al, 2008;Manosroi et al, 2010). Table 3 provides an overview of studies supporting the sericin's antioxidant activity.…”

“…)/day of CSE for a period of 28 days. Deori et al (2016) Rat brain Locally treated (pre-incubation), 0.3% sericin for 30 to 200 min. Kato et al (1998) Mouse fibroblast (L929) cells Locally treated (pre-incubation), 10 and 100 µg/mL sericin.…”