Antioxidant Effects and Physiological Activities of Pumpkin (Cucurbita moschata Duch.) Extract from Different Aerial Parts

Abstract

This study investigated the antioxidant effects and physiological activities of extract from various parts of pumpkin (Cucurbita moschata Duch.) such as leaf, flesh, skin and seed. Different dried parts of C. moschata were boiled in ethanol for three hours, evaporated, and lyophilized with a freeze-dryer. Total polyphenol contents were the highest in leaf (29.62±0.88 mg GAE/g DM). The same result was observed in the measurement of the flavonoid content. The leaf extract of C. moschata had stronger DPPH radical-scavenging activities and ABTS-radical scavenging activities than any other part. Leaf extract of C. moschata was found to have the highest ferric-reducing antioxidant power (FRAP) value. Meanwhile, at 0.1 mg/mL of all extracts higher degree of tyrosinase inhibitory effects than kojic acid was observed (leaf; 49.58%, flesh; 40.20%, skin; 41.30%, seed; 36.79% and kojic acid; 20.70%). The glycation inhibitory activity decreased in the order leaf>skin>flesh>seed. The α-glucosidase inhibitory activity of seed extract was the highest with a value of 63.58% at the concentration of 1 mg/mL.

Enhanced Production of Phenolic Compounds from Pumpkin Leaves by Subcritical Water Hydrolysis

“…Cha ( 14 ) reported that 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of the water extract from pumpkin leaves was 44.6% at the concentration of 20 mg/mL. Kim et al ( 15 ) reported that the ethanol extract of pumpkin leaves had the highest total phenolic contents (29.62 mg gallic acid equivalents/g dry matter), followed by skin (12.08), flesh (7.08), and seed (1.52), and had stronger DPPH-, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)-radical scavenging activities, and ferric reducing antioxidant power (FRAP) value than any other parts.…”

Changes in Phenolic Composition, Antioxidant and Antidiabetic Properties of Jeju Citrus sudachi as Influenced by Maturity

“…체내에는 superoxide dismutase (SOD), glutathione peroxidase, catalase 등의 산화 억제 효소들이 존재하 여 발생된 활성 산소를 소거하지만 [28] 불규칙적인 식습관, 스 트레스, 환경오염 등에 노출된 현대인의 생활습관으로 산화 억제 효소들의 균형이 깨지게 되고 심질환, 당뇨, 신경 질환 등과 같은 만성 질환으로 이어지게 된다 [17]. 현재 다양한 항산 화제 개발이 활발하게 이루어지고 있지만 여러 합성 항산화제 의 부작용이 보고되면서 천연물소재를 이용하여 활성산소를 억제하려는 시도가 활발히 진행 중이다 [13].…”

Changes in Phenolic Metabolites and Biological Activities of Pumpkin Leaves (Cucurbita moschata Duchesne ex Poir.) During Blanching

“…Mkandawire and Masamba ( 10 ) used lemon juice as a blanching medium because the poor rural communities cannot afford commercial food additives. Raw pumpkin leaves showed stronger DPPH-, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)-radical scavenging activities, and ferric reducing antioxidant power (FRAP) compared to the fruit and the seeds ( 11 ). Polyphenols are antioxidants, known as antihypoglycemic agents, proven to lower the fasting blood sugar levels and the risk of type 2 diabetes ( 11 ).…”

“…Raw pumpkin leaves showed stronger DPPH-, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)-radical scavenging activities, and ferric reducing antioxidant power (FRAP) compared to the fruit and the seeds ( 11 ). Polyphenols are antioxidants, known as antihypoglycemic agents, proven to lower the fasting blood sugar levels and the risk of type 2 diabetes ( 11 ). Dietary phenolic compounds inhibit the carbohydrate digestive enzymes, α-amylase and β-glucosidase ( 12 ) and reduce the re-absorption of glucose in the intestine.…”