Antioxidant Effects of Bioactive Compounds Isolated from Pressurized Steam-Treated Corni Fructus and Their Protective Effects Against UVB-Irradiated HS68 Cells

Abstract

This study evaluated the antioxidant and protective effects of bioactive compounds isolated from pressurized steam-treated Corni Fructus (PSC). We had previously tested the protective effects of the furan fraction containing 5-hydroxymethylfurfural (5-HMF), polyphenol fraction containing gallic acid, and iridoid glycoside fraction containing morroniside and loganin. We measured the potency of antioxidant activities of the bioactive compounds isolated from PSC via oxygen radical absorbance capacity (ORAC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays. One fraction in particular (named F-2) not only contained high amounts of phenolics but also had potent antioxidant activities. The protective effects of F-2 were evaluated by measuring the levels of the collagen-degrading enzyme, matrix metalloproteinase-1 (MMP-1), and the marker of collagen biosynthesis, procollagen type I C-peptide (PIP), in UVB-treated HS68 fibroblasts. MMP-1 levels decreased in an F-2 concentration-dependent manner, and PIP secretion from the cultured HS68 cells was significantly higher than that from the UVB-irradiated cultures alone. Further, F-2 attenuated UVB-induced MMP-1 and ameliorated UVB-downregulated collagen type I alpha 1 mRNA expression in HS68 cells. Therefore, F-2 isolated from PSC is a good candidate for the prevention of skin damage from free radicals in various skin conditions.

Inhibition of Digestive Enzymes and Antioxidant Activity of Extracts from Fruits of Cornus alba, Cornus sanguinea subsp. hungarica and Cornus florida–A Comparative Study

“…The iridoid-type compounds are believed to exert less relevant antioxidant activity than phenolic type compounds such as flavonoids and anthocyanins. It was proved that a fractions (1 mg/mL) of Corni fructus containing iridoids like loganin (content 83.07%) and morroniside (content 16.93%) or morroniside alone are less active than the fraction containing 5-hydroxymethylfurfural (content 21.10%), morroniside (content 5.40%) and loganin (content 64.05%) enriched in gallic acid (content 9.45%) [26]. Thus, it is suggested that minor compounds, other than iridoids, determine the antioxidant activity of CF extract.…”

Effects of In Vitro Gastrointestinal Digestion on the Antioxidant Capacity and Anthocyanin Content of Cornelian Cherry Fruit Extract

“…Therefore, a simulated in vitro human digestion model was applied, and the stability of anthocyanins from the investigated fruits, as well as the changes in the antioxidant activity of the digested fruit extract were investigated. Although the anthocyanin profile, the total phenolic content, and the in vitro antioxidant capacity of the Cornelian cherry fruits were already elucidated by several research groups [10,11,17,18,19,20,21], this is the first study dealing with the impact of gastrointestinal digestion on their antioxidant activity and on the bioaccessibility of their anthocyanins.…”

Insight into Seasonal Change of Phytochemicals, Antioxidant, and Anti-Aging Activities of Root Bark of Paeonia suffruticosa (Cortex Moutan) Combined with Multivariate Statistical Analysis

“…The contents of oxypaeoniflorin ( 16) and 1,2,3,4,6-O-pentagalloyl glucose (30) were the highest in LYCM-3 extract. Interestingly, the content of paeonol (50) in the LYCM-1 extract was the highest. The contents of gallic acid (3), paeoniflorin (23), and 1,2,3,4,6-O-pentagalloyl glucose (30) were relatively stable in the four periods, with less than a one-fold difference.…”

“…Gallic acid (3) was one kind of raw material for the synthesis of complex secondary metabolites in the early stage of growth, its content in CM was higher than others in a one-year growth period [41]. The content of paeonol (50) increases with the growth of CM. It is considered that paeonol (50) is one of the marker components in the growth and maturity of CM.…”

“…Gallic acid (3), mudanploside H (37), and luteolin (46) could affect the productions of MMP-1 and PCOL-I in HDF. The iridoid glycoside fraction containing morroniside (4) and loganin (13) could downregulate the expression of MMP-1 mRNA in UVB irradiated HS68 cells [50].…”