Antioxidant effects of bioactive compounds isolated from cordyceps and their protective effects against UVB‐irradiated HaCaT cells

Abstract

Summary Background Excessive free radicals, generated from the metabolic reaction in organisms, have been implicated in many human diseases as well as aging process. Nowadays, many synthetic substances have been developed as anti‐oxidation cosmetic ingredients. However, man‐made antioxidants often have certain toxicity and side effects, which make their application under strict control. Therefore, more and more researchers focus on natural antioxidants because of their advantages. Aims In this study, CE obtained from natural Chinese medicine was used to investigate whether it had antioxidant effect in vitro and repair effect on HaCaT cell damage caused by UVB. Methods UV‐Vis and HPLC were adopted for qualitative and quantitative analysis of CE. We investigated the antioxidant potential of CE by assessing its ABTS+, DPPH•, hydroxyl (OH•), and superoxide anions (O2·-) free‐radical quenching ability. The safety of CE was studied by CCK‐8 assay. To evaluate the anti‐oxidation effect of CE on UVB‐induced damage on HaCaT cells, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) content were tested. Results Experiment data showed that the CE displayed high scavenging ability: ABTS+, DPPH•, OH•, and O2·- quenching rates were 88%, 64%, 94%, and 58%, respectively. Furthermore, after UVB radiation (30 mJ/cm2), adding CE (50‐500 μg/mL) could increase the SOD activity in HaCaT cells and reduce the MDA contents. Conclusions All results illustrate that the CE shows significant antioxidant effect on scavenging free radicals in vitro. Besides, the CE can repair UVB‐induced oxidant damage by improving SOD activity and reducing MDA content.

The Mechanism of Peach Gum Polysaccharide Preventing UVB-Induced Skin Photoaging by Regulating Matrix Metalloproteinanse and Oxidative Factors

“…A large number of studies have reported that natural plant polysaccharides have a good biological protective effect on skin photoaging damage. For example, after HaCaT cells were treated with UVB irradiation (30 mJ/cm 2 ), the addition of Cordyceps polysaccharide was shown to increase the activity of SOD and reduce the content of MDA in the cells, which has a notable effect on antioxidation and free radical scavenging in vitro [ 50 ]. In addition, Wang et al studied the UV protection of seaweed sulfated polysaccharides (HFPS) in HaCaT cells and in zebrafish in vitro and in vivo.…”

Optimization of Antioxidant Activity of Compounds Generated during Ginseng Extract Fermentation Supplemented with Lactobacillus

“…The superoxide anion (O 2 ·− ) scavenging activity of the GS and fermentation samples was assayed using the method described by Tang et al [ 69 ], with slight modifications. For this, 2.25 mL of Tris-HCl buffer (50 mM, pH 8.2), 0.1 mL of sample, and 0.2 mL of catechol solution (3 mM, pre-warmed at 37 °C before use) were mixed.…”

The Large Molecular Weight Polysaccharide from Wild Cordyceps and Its Antitumor Activity on H22 Tumor-Bearing Mice

“…Studies have reported that Cordyceps consists of various chemical constituents, such as nucleosides, lipids, saccharides, mannitol, and amino acids [22]. Modern research has shown that Cordyceps possesses many bioactive functions, such as anti-tumor [23], anti-inflammatory [24], antioxidation [25] and immunomodulation [26] activities. However, its anti-cancer composition is unclear.…”