Antioxidant effects of bovine serum albumin on kinetics, microscopic and oxidative characters of cryopreserved bull spermatozoa

Ashrafi, Iraj; Kohram, Hamid; Tayefi‐Nasrabadi, Hossein

doi:10.5424/sjar/2013113-3870

Cited by 17 publications

(6 citation statements)

References 41 publications

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“…Post-thaw sperm motility and integrity of plasma membrane were better in semen samples containing 1.0% BSA than that in other samples containing (0.5% -1.5% -2% BSA) and also better than the control sample. Our findings are in line with the results reported by (Ashrafi et al, 2013). BSA had beneficial effects on motility, viability, normal morphology, and plasma membrane integrity in bull spermatozoa after a freeze-thaw process.…”

Section: Discussionsupporting

confidence: 93%

The Effects of Bovine Serum Albumin (BSA) Supplementation on Post-thaw Quality of Cryopreserved Bull Semen

Rayan

Anwer²,

El-Badry³

et al. 2023

مجلة سوهاج لشباب الباحثين

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The present work aimed to study the effect of cryopreservation on individual motility, mitochondrial activity, plasma membrane, acrosome, and DNA integrities of bull spermatozoa in response to the addition of different concentrations of bovine serum albumin (BSA) to frozen semen extender. Four adult fertile healthy bulls were enrolled in this study, semen samples were collected weekly from each bull for six successive weeks using an artificial vagina (of 42°C temperature). After semen evaluation, ejaculates from each bull were diluted in a Tris-based buffer egg yolk extender and cooled to 5C. The Semen extender was supplemented by five different concentrations of BSA;0 (as a control group), 0.5, 1, 1.5, and 2 g/100 ml. Samples were processed for cryopreservation. Each cooled ejaculate was split into five equal aliquots and diluted with BSA supplemented extender; then semen was packaged in 0.25 ml straws and placed 4 cm above liquid nitrogen before being plunged into a liquid nitrogen tank and stored. Frozen straws were thawed in a water bath at 37°C for 30 sec and then evaluated for assessment of individual motility, mitochondrial activity, and plasma membrane and acrosome integrities. The results of the present study showed that post-thaw sperm motility and integrity of plasma membrane were significantly better (P<0.05) in semen samples containing 1.0% BSA (60.00±2.88 and 59.67±1.45 respectively) than in control sample (52.33±1.45 and 53.67±1.85, respectively). Moreover, BSA 1%-supplemented group had better motility and plasma membrane integrity than the other three groups; supplemented with BSA 0.5%-, BSA Article info.

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Section: Discussionsupporting

confidence: 93%

The Effects of Bovine Serum Albumin (BSA) Supplementation on Post-thaw Quality of Cryopreserved Bull Semen

Rayan

Anwer²,

El-Badry³

et al. 2023

مجلة سوهاج لشباب الباحثين

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“…When semen is required within a short period of time after collection for AI or IVF, chilled storage is considered for sperm preservation (Câmara et al, 2011;King et al, 2004). However, the full potential of semen utilization, particularly for commercial application purposes, relies on using cryopreserved doses (Ashrafi, Kohram, & Tayefi-Nasrabadi, 2013;Faigl et al, 2012). Nevertheless, liquidchilled storage of semen has been reported to deleteriously affect both sperm motility and morphology and, hence, sperm fertilizing capacity over preservation time (Alam, Yeashmin, Bari, & Mishra, 2005;Kasimanickam, Kasimanickam, Thatcher, Nebel, & Cassell, 2007;Munsi, Bhuiyan, Majumder, & Alam, 2007).…”

Section: Introductionmentioning

confidence: 99%

Influence of omega‐3 incorporation in sperm preservation medium on physical and kinematic properties of chilled and cryopreserved ram spermatozoa

Rateb

2018

Reprod Domestic Animals

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Two experiments were carried out to investigate the efficiency of supplementing sperm preservation medium with omega-3 polyunsaturated fatty acids on improving liquid-chilled storage and cryopreservation capacity of ram spermatozoa. Ejaculates (n = 100) were collected from five adult rams, Ovis aries, by an artificial vagina twice weekly throughout the period February-April, 2017. After initial evaluation, ejaculates of each collection session from the same males were pooled, diluted (1:10) with Tris-citric acid egg yolk extender, and were further split into five aliquots using a split-sample technique. The first aliquot served as control (omega-free), whereas the other four portions were supplemented with 0.1, 0.2, 0.3 or 0.4 mM omega-3, respectively (T ). Thereafter, the diluted specimens were stored at 4°C for 48 hr, during which sperm physical and morphometric properties were evaluated along with oxidative stress indices (T , T ). Omega-3 levels that efficiently mitigated the detrimental effects of chilled preservation, and maintained preservation aptitude of spermatozoa were further investigated for sperm cryosurvival against control (untreated). Post-thaw physical and kinematic properties of spermatozoa, in all groups, were objectively evaluated by a computer-assisted sperm analysis (CASA) system. The results showed that, at 48 hr of chilled storage, supplementing preservation medium with 0.4 mM omega-3 was positively correlated (p < 0.01) with each of progressive motility, live sperm, intact acrosome and intact cell membrane (r = 0.83, 0.85, 0.85, 0.89, respectively). Furthermore, a positive correlation (p < 0.01) was observed between inclusion of omega-3 in cryopreservation medium and each of post-thaw total sperm motility, progressive motility, live sperm, normal sperm, intact acrosome, intact cell membrane, VCL, VSL, VAP, ALH and STR (r = 0.76, 0.84, 0.79, 0.90, 0.89, 0.91, 0.61, 0.73, 0.65, 0.78 and 0.60, respectively). These results accentuate efficiency of supplementing the diluent with omega-3 fatty acids on improving chilled and cryopreservation aptitude of ram spermatozoa.

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“…Nevertheless, improving semen quality during cold storage still appears to be a challenge in sheep industry (Hashem et al, 2017). In the meantime, the full potential of semen utilization relies on using it in the cryopreserved form (Ashrafi et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Influence of Supplementing Ram Semen Diluent With Reduced Glutathione on Oxidative Stress Indices and Quality Attributes of Chilled and Cryopreserved Spermatozoa

Khalifa

2023

Egyptian Journal of Animal Production

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This study was carried out to evaluate the efficiency of supplementing ram semen diluent with glutathione (GSH) on liquid-chilled and cryo-storage capacities of spermatozoa. Twenty ejaculates were collected from 5 Barki rams, 4 ejaculates each, during late-January and mid-February using an artificial vagina. The pooled specimens of each collection session were diluted (1:10) with Tris-citric egg yolk extender, and were split into 5 aliquots. The first aliquot served as control (GSH-free), whereas the other 4 aliquots were supplemented with 0.1, 0.2, 0.3 or 0.4 mMGSH. Thereafter, the samples were stored at 4°C for 3 h whereat each sample was subdivided into two portions. The first portion was maintained at 4°C for the subsequent 48 h during which sperm traits were assessed alongside oxidative stress indicators, whereas a chilled-glycerolized base diluent was added to the other portion and was further processed for cryopreservation. Post-thaw physical and kinematic properties of spermatozoa were evaluated by a computer-assisted sperm analysis (CASA) system, and sperm DNA fragmentation level within each group was also determined by fluorescent imaging. The results showed that GSH supplementation reduced (P<0.05) the oxidative stress on spermatozoa and maintained their physical and morphological criteria over the 48h period of chilled storage in a dose-depending pattern. Moreover, the 0.4 mM level of GSH improved (P<0.05) post-thaw traits and kinematics of ram sperm while decreasing (P<0.05) DNA fragmentation level compared to the control specimens. These findings imply glutathione's powerful potential as an exogenous antioxidant supplement in ram sperm medium which could be of great significance for further application in semen processing and IVF workflows.

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Antioxidant effects of bovine serum albumin on kinetics, microscopic and oxidative characters of cryopreserved bull spermatozoa

Cited by 17 publications

References 41 publications

The Effects of Bovine Serum Albumin (BSA) Supplementation on Post-thaw Quality of Cryopreserved Bull Semen

The Effects of Bovine Serum Albumin (BSA) Supplementation on Post-thaw Quality of Cryopreserved Bull Semen

Influence of omega‐3 incorporation in sperm preservation medium on physical and kinematic properties of chilled and cryopreserved ram spermatozoa

Influence of Supplementing Ram Semen Diluent With Reduced Glutathione on Oxidative Stress Indices and Quality Attributes of Chilled and Cryopreserved Spermatozoa

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