Antioxidant effects of dihydrocaffeic acid in human EA.hy926 endothelial cells

Huang, Junjun; Paulis, Tomas de; May, James M.

doi:10.1016/j.jnutbio.2004.07.002

Cited by 98 publications

(54 citation statements)

References 46 publications

(54 reference statements)

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“…Huang et al, (2004) found a significant increase in protein expression and activity of eNOS enzyme in EA.hy926 cell line after incubation with dihydrocaffeic aicd (caffeic acid metabolite) at different concentrations (0-200 µM) for 18 hours. The effect of resveratrol, epigallocatechingallate and epicatechingallate on eNOS enzyme was investigated on EA.hy926 cell line at different concentrations (0-100 µM) for 24 hours.…”

Section: Discussionmentioning

confidence: 94%

“…EA.hy296 cell line incubated with red wine polyphenol extract (100-600 µg/ml for 18 hours produced a significant increase in NO production [38]. Dihydrocaffeic acid (caffeic acid metabolite) at different concentrations (0-200 µM) significantly increased the production of NO after being added to EA.hy926 cells for 18 hours in a dose-dependent manner [39]. Another study was performed on NO production after incubating the bovine pulmonary artery endothelial cell line with 50 and 100 µM pomegranate juice for 24 and 48 hours.…”

Section: Discussionmentioning

confidence: 99%

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Punicalagin Induce the Production of Nitric Oxide and Inhibit Angiotensin Converting Enzyme in Endothelial Cell Line EA.hy926

Omar¹,

Yousr²,

Howell³

2016

ujph

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Introduction: punicalagin, a hydrolysable tannin polyphenol from pomegranate, reported to have a protective effect against many diseases due to its high antioxidant and free radical scavenging activities. Aim, this study investigated the potential antihypertensive activity of punicalagin in human derived EA.hy926 endothelial cell model, via two mechanisms. In first mechanism, punicalagin enhance the nitric oxide production through scavenging reactive oxygen specious (ROS) and activating the endothelial nitric oxide synthase enzyme (eNOS). In second mechanism, punicalagin showed effect activity by inhibiting the angiotensin converting enzyme (ACE). Methods: The effect of different concentrations (1-100 µM) of punicalagin on EA.hy926 cells was measured using MTT assay. Induction of ROS was done using Ang II and scavenging activity of punicalagin was assessed by flow cytometer and fluorimeter. NO production was measured in order to determine the effective dose of punicalagin followed by measuring the ability of punicalagin to induce eNOS activity and the enzyme expression by Western blotting cellular Ca 2+ concentration and ACE inhibition were also determined. Results: Punicalagin (1-60 µM) reduced ROS production in EA.hy926, which induced by added angiotensin II, as shown by flow cytometry and by fluorometry. In addition, at the same concentration the nitric oxide production was increased in a dose-dependent manner due to increased eNOS activation. The activation of eNOS enzyme was promoted by an increase of cellular calcium concentration at the tested concentrations. Examined punicalagin concentrations significantly inhibited ACE activity, possibly due to zinc binding. Conclusion: punicalagin clearly exhibits the potential for reducing hypertensive activity by a dual mechanism of nitric oxide synthase induction by increasing nitric oxide levels, and ACE inhibition.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Punicalagin Induce the Production of Nitric Oxide and Inhibit Angiotensin Converting Enzyme in Endothelial Cell Line EA.hy926

Omar¹,

Yousr²,

Howell³

2016

ujph

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“…Overall, phenol derivatives may exert biological activities. Dihydrocaffeic acid, the reduced derivative of caffeic acid, has well-known and higher antioxidant activity than its precursor (35,36). The screening of strains based on enzymatic activities toward hydroxycinnamic acids may allow the selection of starter cultures with both optimal technology and functional features.…”

Section: Resultsmentioning

confidence: 99%

Hydroxycinnamic Acids Used as External Acceptors of Electrons: an Energetic Advantage for Strictly Heterofermentative Lactic Acid Bacteria

Filannino

Gobbetti

Angelis

et al. 2014

Appl Environ Microbiol

103

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The metabolism of hydroxycinnamic acids by strictly heterofermentative lactic acid bacteria (19 strains) was investigated as a potential alternative energy route. Lactobacillus curvatus PE5 was the most tolerant to hydroxycinnamic acids, followed by strains of Weissella spp., Lactobacillus brevis, Lactobacillus fermentum, and Leuconostoc mesenteroides, for which the MIC values were the same. The highest sensitivity was found for Lactobacillus rossiae strains. During growth in MRS broth, lactic acid bacteria reduced caffeic, p-coumaric, and ferulic acids into dihydrocaffeic, phloretic, and dihydroferulic acids, respectively, or decarboxylated hydroxycinnamic acids into the corresponding vinyl derivatives and then reduced the latter compounds to ethyl compounds. Reductase activities mainly emerged, and the activities of selected strains were further investigated in chemically defined basal medium (CDM) under anaerobic conditions. The end products of carbon metabolism were quantified, as were the levels of intracellular ATP and the NAD ؉ /NADH ratio. Electron and carbon balances and theoretical ATP/glucose yields were also estimated. When CDM was supplemented with hydroxycinnamic acids, the synthesis of ethanol decreased and the concentration of acetic acid increased. The levels of these metabolites reflected on the alcohol dehydrogenase and acetate kinase activities. Overall, some biochemical traits distinguished the common metabolism of strictly heterofermentative strains: main reductase activity toward hydroxycinnamic acids, a shift from alcohol dehydrogenase to acetate kinase activities, an increase in the NAD ؉ /NADH ratio, and the accumulation of supplementary intracellular ATP. Taken together, the above-described metabolic responses suggest that strictly heterofermentative lactic acid bacteria mainly use hydroxycinnamic acids as external acceptors of electrons.

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“…Phenol derivatives may also exert biological activities. Dihydrocaffeic acid, the reduced derivative of caffeic acid, has well-known higher antioxidant activity than its precursor (58,59). Thus, the choice of FLAB starter strains with specific enzymatic activities involving phenolic acids may have a promising role in food fermentation.…”

Section: Discussionmentioning

confidence: 99%

Metabolism of Fructophilic Lactic Acid Bacteria Isolated from the Apis mellifera L. Bee Gut: Phenolic Acids as External Electron Acceptors

Filannino

Cagno

Addante

et al. 2016

Appl Environ Microbiol

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Fructophilic lactic acid bacteria (FLAB) are strongly associated with the gastrointestinal tracts (GITs) of Apis mellifera L. worker bees due to the consumption of fructose as a major carbohydrate. Seventy-seven presumptive lactic acid bacteria (LAB) were isolated from GITs of healthy A. mellifera L. adults, which were collected from 5 different geographical locations of the Apulia region of Italy. Almost all of the isolates showed fructophilic tendencies: these isolates were identified as Lactobacillus kunkeei (69%) or Fructobacillus fructosus (31%). A high-throughput phenotypic microarray targeting 190 carbon sources was used to determine that 83 compounds were differentially consumed. Phenotyping grouped the strains into two clusters, reflecting growth performance. The utilization of phenolic acids, such as p-coumaric, caffeic, syringic, or gallic acids, as electron acceptors was investigated in fructose-based medium. Almost all FLAB strains showed tolerance to high phenolic acid concentrations. p-Coumaric acid and caffeic acid were consumed by all FLAB strains through reductases or decarboxylases. Syringic and gallic acids were partially metabolized. The data collected suggest that FLAB require external electron acceptors to regenerate NADH. The use of phenolic acids as external electron acceptors by the 4 FLAB showing the highest phenolic acid reductase activity was investigated in glucose-based medium supplemented with p-coumaric acid. Metabolic responses observed through a phenotypic microarray suggested that FLAB may use p-coumaric acid as an external electron acceptor, enhancing glucose dissimilation but less efficiently than other external acceptors such as fructose or pyruvic acid. Fructophilic lactic acid bacteria (FLAB), described only recently (1-9), belong to a special group of lactic acid bacteria (LAB) that prefer fructose instead of glucose as a carbon source. These bacteria have been isolated from specific ecological fructose-rich niches such as flowers, fruits, and fermented food based-fruits and, only recently, from the gastrointestinal tracts (GITs) of several insects (e.g., bumblebees, honeybees, tropical fruit flies, and Camponotus ants), which have a fructose-based diet (4, 10, 11). Among these insects, social honeybees are those of greater interest because of their economic and ecological importance for honeybee products (e.g., propolis, royal jelly, honey, and pollen) and especially for crop pollination. Despite a global increase in the population of domesticated bees according to the FAO data (12), honeybees are facing growing adversity. Localized declines in bee populations have occurred in many European countries (13-15). To understand and to prevent the decrease, several studies have been addressed to investigate the symbiotic and pathogenic microbial interactions (16-19). The observations made as of to date indicate the honeybees' GITs harbor a core microbiota dissimilar to those of other animals, including humans (16)(17)(18)(19)(20)(21)(22)(23)(24). FLAB are strongly as...

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Antioxidant effects of dihydrocaffeic acid in human EA.hy926 endothelial cells

Cited by 98 publications

References 46 publications

Punicalagin Induce the Production of Nitric Oxide and Inhibit Angiotensin Converting Enzyme in Endothelial Cell Line EA.hy926

Punicalagin Induce the Production of Nitric Oxide and Inhibit Angiotensin Converting Enzyme in Endothelial Cell Line EA.hy926

Hydroxycinnamic Acids Used as External Acceptors of Electrons: an Energetic Advantage for Strictly Heterofermentative Lactic Acid Bacteria

Metabolism of Fructophilic Lactic Acid Bacteria Isolated from the Apis mellifera L. Bee Gut: Phenolic Acids as External Electron Acceptors

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