Antioxidant effects of exogenous polyamines in damage of lysosomes inflicted by xanthine oxidase or stimulated polymorphonuclear leucocytes

Kafy, A. M. L.; Lewis, David

doi:10.1007/bf01968093

Cited by 11 publications

(3 citation statements)

References 24 publications

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“…The lysis of lysosomes, inducing inflammation, by active oxygen species of guinea pig polymorphonuclear leukocytes (63) and the inflammatory effects of ozone (34) were successfully prevented by polyamines. These observations indicate that polyamines may be important in the reduction of inflammatory responses (13) and thus indirectly reduce bronchial hyperreactivity.…”

Section: Polyamines and Pulmonary Inflammationmentioning

confidence: 99%

“…2) The whole lung, specifically the pulmonary epithelium, is uniquely exposed to a high oxygen tension and numerous inhaled environmental agents (e.g., ozone, cigarette smoke). It has been demonstrated that polyamines can protect against radicals in two different ways: first, by direct quenching of free radicals (34,46,63,79) and second, by condensing the DNA, in other words, by protecting the genomic information (34). Polyamines might thus act as one of the many defense lines against free radical attack in the lungs, protecting against DNA strand breaks due to reactive oxygen species (46,69,70).…”

Section: General Conclusionmentioning

confidence: 99%

“…A few mechanisms may play a role in this function. Polyamines can simply bind to negatively charged allergens, polyamines can interact with the inflammatory system (36,164), and/or polyamines can reduce inflammatory responses through the scavenging of radicals (34,63).…”

Section: General Conclusionmentioning

confidence: 99%

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Polyamines in the lung: polyamine uptake and polyamine-linked pathological or toxicological conditions

Hoet

Nemery

2000

American Journal of Physiology-Lung Cellular and Molecular Phys

107

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The natural polyamines putrescine, cadaverine, spermidine, and spermine are found in all cells. These (poly)cations exert interactions with anions, e.g., DNA and RNA. This feature represents their best-known direct physiological role in cellular functions: cell growth, division, and differentiation. The lung and, more specifically, alveolar epithelial cells appear to be endowed with a much higher polyamine uptake system than any other major organ. In the lung, the active accumulation of natural polyamines in the epithelium has been studied in various mammalian species including rat, hamster, rabbit, and human. The kinetic parameters (Michaelis-Menten constant and maximal uptake) of the uptake system are the same order of magnitude regardless of the polyamine or species studied and the in vitro system used. Also, other pulmonary cells accumulate polyamines but never to the same extent as the epithelium. Although different uptake systems exist for putrescine, spermidine, and spermine in the lung, neither the nature of the carrier protein nor the reason for its existence is known. Some pulmonary toxicological and/or pathological conditions have been related to polyamine metabolism and/or polyamine content in the lung. Polyamines possess an important intrinsic toxicity. From in vitro studies with nonpulmonary cells, it has been shown that spermidine and spermine can be metabolized to hydrogen peroxide, ammonium, and acrolein, which can all cause cellular toxicity. In hyperoxia or after ozone exposure, the increased polyamine synthesis and polyamine content of the rat lung is correlated with survival of the animals. Pulmonary hypertension induced by monocrotaline or hypoxia has also been linked to the increased polyamine metabolism and polyamine content of the lung. In a small number of studies, it has been shown that polyamines can contribute to the suppression of immunologic reactions in the lung.

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Section: Polyamines and Pulmonary Inflammationmentioning

confidence: 99%

Section: General Conclusionmentioning

confidence: 99%

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Polyamines in the lung: polyamine uptake and polyamine-linked pathological or toxicological conditions

Hoet

Nemery

2000

American Journal of Physiology-Lung Cellular and Molecular Phys

107

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Protective effect of spermine on DNA exposed to oxidative stress

et al. 1995

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Pathological conditions that cause oxidative stress can affect DNA integrity. The aim of this research was to study the protective effect of spermine against DNA damage induced by an oxygen-radical generating system. Deoxyguanosine and DNA were separately dissolved in phosphate buffer and incubated for 1 h at 40 degrees C in the presence of 50 mM H2O2/10 mM ascorbic acid. Single nucleosides and their products of oxidation were then obtained by enzymatic digestion of DNA. The compounds were separated by micellar electrokinetic capillary chromatography (MECC) with SDS-modified mobile phase and detected at 254 nm. Two major products of DNA oxidation have been identified as derivatives of deoxyguanosine with electrophoretic properties different from 8-hydroxy-2'-deoxyguanosine. When the oxidation of DNA was carried out in the presence of 0.1 mM spermine, the formation of the two by-products of deoxyguanosine was markedly reduced. On the contrary, spermine did not prevent the oxidation of deoxyguanosine alone, suggesting that the polyamine should be bound to the DNA strands to exert its antioxidative effect.

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Antioxidative effects of polyamines

Løvaas

1991

J Americ Oil Chem Soc

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The antioxidative effects of spermine, spermidine and putrescine were determined by measurement of primary and secondary oxidation products of polyunsaturated fatty acids, using gas and liquid chromatography as well as spectrophotometric recordings. It was demonstrated that polyamines inhibit the oxidation of polyunsaturated fatty acids, a-tocopherol and carotenoid pigments. Both linear and nonlinear dose/response relationships have been observed. The efficiency of a given polyamine was correlated with the number of amine groups in the molecule. Spermine was, thus, more efficient than spernfidine, which in turn had a higher efficiency than putrescine. The relative antioxidative effect was as follows: spermine (100.0), spermidine (61.0), putrescine (23.0), ethoxyquin (27.6), ascorbyl palmitate (18.3), octyl gallate (7.9), tert butylhydroquinone (6.3), butylated hydroxyanisole (3.6) and a-tocopherol (3.4).

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Antioxidant effects of exogenous polyamines in damage of lysosomes inflicted by xanthine oxidase or stimulated polymorphonuclear leucocytes

Cited by 11 publications

References 24 publications

Polyamines in the lung: polyamine uptake and polyamine-linked pathological or toxicological conditions

Polyamines in the lung: polyamine uptake and polyamine-linked pathological or toxicological conditions

Protective effect of spermine on DNA exposed to oxidative stress

Antioxidative effects of polyamines

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