Antioxidant Effects of Melatonin and Coenzyme Q10 on Oxidative Damage Caused by Single-Dose Ochratoxin A in Rat Kidney

Yenilmez, A.; Işikli, Burhanettin; Aral, Erinc; Değirmenci, İrfan; Sutken, Emine; Bayçu, Cengiz

doi:10.4077/cjp.2010.amk073

Cited by 19 publications

(16 citation statements)

References 30 publications

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“…Yenilmez et al . investigated the effects of a relatively high single-dose of OTA and the antioxidant effects of Mel and CoQ 10 on OTA-induced oxidative damage in rats [166]. Male Sprague-Dawley rats were divided into four groups of seven rats each: control, OTA, Mel + OTA and CoQ 10 + OTA groups.…”

Section: Ochratoxin a Toxicity: The Effect Of Antioxidant Substancmentioning

confidence: 99%

Toxicity of Ochratoxin A and Its Modulation by Antioxidants: A Review

Sorrenti

Giacomo

Acquaviva

et al. 2013

Toxins

170

105

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Ochratoxin A (OTA) is a mycotoxin involved in the development of different types of cancers in rats, mice and humans. A growing number of in vitro and in vivo studies has been collected and has described evidence compatible with a role for oxidative stress in OTA toxicity and carcinogenicity. Because the contribution of the oxidative stress response in the development of cancers is well established, a role in OTA carcinogenicity is plausible. Several studies have been performed to try to counteract the adverse effects of oxygen radicals generated under OTA-exposure. A number of molecules with various antioxidant properties were tested, using in vivo or in vitro models. Protection against OTA-induced DNA damage, lipid peroxidation, as well as cytotoxicity were observed, further confirming the link between OTA toxicity and oxidative damage. These studies demonstrated that antioxidants are able to counteract the deleterious effects of chronic consumption or exposure to OTA and confirmed the potential effectiveness of dietary strategies to counteract OTA toxicity.

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Section: Ochratoxin a Toxicity: The Effect Of Antioxidant Substancmentioning

confidence: 99%

Toxicity of Ochratoxin A and Its Modulation by Antioxidants: A Review

Sorrenti

Giacomo

Acquaviva

et al. 2013

Toxins

170

105

View full text Add to dashboard Cite

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“…Ochratoxin A is in part effective by triggering apoptosis [5,6,9,10,[15][16][17][18][19][20][21][22][23][24][25][26]. Mechanisms involved in the stimulation of apoptosis by ochratoxin A include oxidative stress [18,27,28] and Ca 2+ entry [29]. Similar to apoptosis of nucleated cells, erythrocytes may undergo suicidal death or eryptosis [30].…”

Section: Introductionmentioning

confidence: 99%

Enhanced Apoptotic Death of Erythrocytes Induced by the Mycotoxin Ochratoxin A

Jilani

Lupescu

Zbidah

et al. 2012

Kidney Blood Press Res

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Background: The mycotoxin ochratoxin A, an agent responsible for endemic Balkan nephropathy is known to trigger apoptosis and thus being toxic to several organs including the kidney. The mechanisms involved in ochratoxin A induced apoptosis include oxidative stress. Sequelae of ochratoxin intoxication include anemia. Similar to apoptosis of nucleated cells, erythrocytes may undergo suicidal cell death or eryptosis, which is characterized by cell shrinkage and cell membrane scrambling resulting in phosphatidylserine-exposure at the cell surface. Eryptosis could be triggered by Ca²⁺-entry through oxidant sensitive unspecificcation channels increasing cytosolic Ca²⁺ activity ([Ca²⁺]_i). The Ca²⁺-sensitivity of cell membrane scrambling could be enhanced and eryptosis thus triggered by ceramide. The removal of suicidal erythrocytes may lead to anemia. Moreover, eryptotic erythrocytes could adhere to the vascular wall thus impeding microcirculation. The present study explored, whether ochratoxin A stimulates eryptosis. Methods: Fluo3-fluorescence was utilized to determine [Ca²⁺]_i, forward scatter to estimate cell volume, annexin-V-binding to identify phosphatidylserine-exposing cells, fluorescent antibodies to detect ceramide formation and hemoglobin release to quantify hemolysis. Moreover, adhesion to human vascular endothelial cells (HUVEC) was determined utilizing a flow chamber. Results: A 48 h exposure to ochratoxin A was followed by significant increase of Fluo3-fluorescencei (≥ 2.5 µM), increase of ceramide abundance (10 µM), decrease of forward scatter (≥ 5 µM) and increase of annexin-V-binding (≥ 2.5 µM). Ochratoxin A exposure slightly but significantly enhanced hemolysis (10 µM). Ochratoxin (10 µM) enhanced erythrocyte adhesion to HUVEC. Removal of extracellular Ca²⁺ significantly blunted, but did not abrogate ochratoxin A-induced annexin V binding. Conclusions: Ochratoxin A triggers suicidal erythrocyte death or eryptosis, an effect partially but not fully due to stimulation of Ca²⁺-entry.

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“…Several antioxidants have been applied to provide protection against OA-induced hepato-renal toxicity (Yenilmez et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Mushroom Insoluble Non-Starch Polysaccharides (MINSP) Attenuate Ochratoxin A-Oxidative Stress and Hepato-Renal Dysfunctions in Rat

Nada¹,

Omara²,

Amra³

et al. 2014

Virol Mycol

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The aim of the present study was to investigate the effect of mushroom insoluble non-starch polysaccharides (MINSP) on Ochratoxin A-induced hepato-renal damage in rat. MINSP (75 and 150 mg/kg) administered daily for 15 days in concomitantly with Ochratoxin A (OA) (1.7 mg/Kg), intraperitoneal (i.p). Normal groups treated with MINSP showed significant decrease in serum levels of -glutamyle transferase (GGT),aminotransferases (ALT & AST), creatinine, uric acid, total antioxidant capacity (TAC), tumor necrosis factor alpha (TNF-α ) and carcinoembryonic antigen (CEA). In liver and kidney homogenates there were significant decreases in malonaldehyde (MDA), and nitric oxide (NO); while superoxide dismutase (SOD) content increased after treatment with MINSP. OA-treated rats showed significant elevation in serum ALT, AST, TNF-α, CEA, Uric acid, creatinine, MDA and NO and reduction of SOD level in hepatic and renal tissues. The combined treatment with MINSP + OA significantly ameliorated the tested parameters when compared with OA-treated group. It improved the antioxidant activity of the liver and kidney in dosedependent manner. Histopathological examination revealed that MINSP administration protected hepatocytes and renal tissues from the damage induced by OA. Immunohistochemical staining of iNOS expression and DNA damaged area decreased in liver and kidney tissues after administration of MINSP. Conclusion: MINSP are fat replacer in processing low fat diet. MINSP represent a good functional food and have detoxifying effect against ochratoxicosis in rat.

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Antioxidant Effects of Melatonin and Coenzyme Q10 on Oxidative Damage Caused by Single-Dose Ochratoxin A in Rat Kidney

Cited by 19 publications

References 30 publications

Toxicity of Ochratoxin A and Its Modulation by Antioxidants: A Review

Toxicity of Ochratoxin A and Its Modulation by Antioxidants: A Review

Enhanced Apoptotic Death of Erythrocytes Induced by the Mycotoxin Ochratoxin A

Mushroom Insoluble Non-Starch Polysaccharides (MINSP) Attenuate Ochratoxin A-Oxidative Stress and Hepato-Renal Dysfunctions in Rat

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