Antioxidant Effects of Muehlenbeckia Hastulata J. (Polygonaceae) Extracts

Abstract

The antioxidant properties of different extracts of quilo (Muehlenbeckia hastulata J., Poligonaceae) herb from Valparaíso region in Chile were evaluated. Antioxidant activity was assessed in three different model systems. Antioxidant models were examined by measuring the scavenging activity of extracts on 2,2-diphenyl-1-pycrylhydrazil (DPPH • ). Moreover extracts were evaluated by ferric reducing antioxidant power (FRAP) and the total peroxyl radical-trapping potential (TRAP). The amount of dried extract, the content of total phenolics, flavonoids, hydrolyzed sugar and anthraquinones were also determined. Extracts pertaining to stem and root of the plants were the significant activities DPPH radical scavenging (IC 50 0.74 mg/mL to 1.33 mg/mL the more active extracts). The activities and TRAP and FRAP high values in extracts of EtOAc (1.423 and 1.540 mM TEAC) and ethanol (245.26 and 309.04 mM TEAC) stem and root of the plant are higher.The observed differences in antioxidant activity among the different parts of quilo could be partially explained by the levels of phenolics compounds present in the root extract of EtOAc (0.023 mg GAE/g dry extract), flavonoids in the leaves of EtOAc extract (99.12 mg QE/g dry extract), hydrolyzed sugar in the ethanol extract (21.87, 14.85 and 14.32 mg GES/ g dry extract of leaf, stem and roots, respectively) and anthraquinones in the EtOAc extracts (2.50, 1.43 and 0.84 mg EE/ g dry extract of leaf, stem and roots, respectively) .

Antioxidant and anti cancer activities of brown and red seaweed extracts from Chilean coasts

“…The presence of both compounds in the dichloromethane extract is unusual given that this solvent extracts apolar compounds such as terpenoids (Seidel, 2005). We attribute the presence of polyphenols on dichloromethane extracts to a possible susceptibility of the measurement to false positives (Mellado, 2012a). It is important to mention that there has been no previous research quantifying the flavonoid or anthraquinone contents in any of the species presented in this study.…”

In vitro antioxidant and antiproliferative effect of the extracts of Ephedra chilensis K Presl aerial parts

“…This estimation was carried out using the protocol of Arvouet-Grand et al adapted by Mellado et al [14]. One mL of 2% w / v aluminium trichloride (AlCl 3 ) in ethanol was mixed with the same volume of the extract solution in ethanol (1.0 mg/mL).…”

Antioxidant Activity of Anthraquinones Isolated From Leaves of Muehlenbeckia Hastulata (J.E. Sm.) Johnst. (Polygonaceae)

“…The organic layer was separated and dried with Mg 2 SO 4 and subsequently filtered, evaporated and purified by flash chromatography on silica gel (0.063 to 0.200 mesh) with a hexane -EtOAc gradient (1% to 50%). 15 . A volume (0.1 mL) of the samples (from 0 to 50 mg/mL of the isolated compound) was mixed with 2.9 mL 50 μM DPPH • solution freshly prepared in ethanol.…”

“…Besides, hexane, dichloromethane and ethanol extracts of the aerial parts show an analgesic effect and weak antibacterial activity against Escherichia coli, Klebsiella pneumoniae, Salmonella aviatum, Salmonella aeruginosa, Staphylococcus aureus, Micrococcus flavus and Bacillus subtilis. Finally, recent studies of the antioxidant activity of Muehlenbeckia hastulata extracts report that the ethyl acetate extract from leaves has a high antioxidant activity (IC 50 5.79 mg/mL) compared to gallic acid (10.52 mg/mL) 5,[14][15] .…”