Antioxidant effects of polyphenolic compounds in through the distillation of Lonicera japonica & Chenpi Extract and anti-inflammation on skin keratinocyte

Abstract

The polyphenols in Lonicera japonica & Chenpi distillation extract (LCDE) were thoroughly examined for their antioxidant and anti-inflammatory properties. Polyphenol compounds in LCDE were analyzed for five peaks using high-performance liquid chromatography (HPLC) combined with mass spectrometry (MS) and determined. Five polyphenolic compounds were identified from the samples and MS data. Ultrafiltration with LC analysis was used to investigate the ability of bioactive compounds to target DPPH. As a result, it was confirmed that the major compounds exhibited a high binding affinity to DPPH and could be regarded as antioxidant-active compounds. In addition, the anti-inflammatory effect of LCDE was confirmed in vitro, and signal inhibition of anti-inflammation cytokines, MAPK and NF-kB pathways was confirmed. Finally, Molecular docking analysis supplements the anti-inflammatory effect through the binding affinity of selected compounds and inflammatory factors. This study will be utilized to give fundamental information for the application of LCDE to the pharmaceutical and pharma cosmetics industries in addition to information on efficient screening techniques for other medicinal plants.