Antioxidant effects of propolis on carp Cyprinus carpio exposed to arsenic: biochemical and histopathologic findings

Abstract

Propolis, a resinous material produced by worker bees from the leaf buds and exudates of plants, is reported to possess various therapeutic properties. The aim of this study was to investigate the effects of propolis on biochemical parameters and histopathologic findings in carp Cyprinus carpio L. exposed to arsenic. A sublethal concentration of arsenic (0.01 mg l-1) and/or 10 mg l-1 propolis were administered to fish for 1 wk. Catalase (CAT) activities and malondialdehyde (MDA) levels were determined in liver, gill and muscle tissues in control, arsenic only, propolis only and arsenic+propolis treatment groups. Results showed that CAT activity decreased in the arsenic group compared to the control and propolis groups. CAT activity in the arsenic+propolis group was significantly higher compared to the arsenic group. MDA levels in fish exposed to 0.01 mg l-1 arsenic significantly increased compared to the control group. However, MDA levels in the arsenic+propolis group were significantly lower compared to the arsenic group. Histopathological changes in the liver, gill and muscle tissues of carp were examined by light microscopy: various changes were observed in all tissues of fish in the arsenic group. Propolis showed important antioxidant effects against arsenic toxicity in all fish tissues.

Pathological and Biochemical Studies on some Antimicrobials in Clarias garipeinus Fish Infected with Aeromonas hydrophila

“…The significant decrease in MDA in Groups 5 and 6 might be related to flavonoids, which responsible for the antioxidant activity of propolis [35]. Propolis ameliorated the elevation in MDA of Cyprinus carpio exposed to chromium [41] and had antioxidant effects [3]. Our investigation showed a significant increase in the immunological parameters (phagocytic ratio and phagocytic index) in infected and noninfected groups treated with propolis (Group 3) and (Group 5) also, in combination with norfloxacin (Group 6) ( Table 2).…”

“…Propolis is a natural honeybees' product, which contain a variety of different chemical compounds as polyphenols (flavonoid aglycones, phenolic and their esters, phenolic aldehydes, alcohols and ketones), steroids, amino acids and inorganic compounds [2]. Propolis is aresinous material produced by worker bees from leaf bud and exudates of plants [3]. It has many different pharmacological activities as antiinflammatory, antiviral, antioxidant, antifungal, antibiotic and immunostimulant effects [4].…”

“…AST: aspartate aminotransferase, 2 ALT: alanine aminotransferase,3 MDA: Malondialdhyde, Means with different letters at the same row were significant P<0.05.…”

“…RBCs: Red blood corpuscle, 2 Hb: Haemoglobin,3 PCV%: Packed cell volume,4 WBCs: White blood corpuscle Means with different letters at the same column (1 st and 10 th days post treatment separately) were significant P<0.05.…”

Utilização de própolis e probiótico em dietas para leitões recém-desmamados

“…A utilização da própolis em dietas animais tem demonstrado efeitos positivos sobre o desempenho de suínos, frangos de corte, coelhos, vacas leiteiras, caprinos e peixes (BIAVATTI et al, 2003;ÇETIN et al, 2010;DANESHMAND et al, 2015;DENLI et al, 2005;EYNG et al, 2014;FRANCO et al, 2007;FREITAS et al, 2011;GARCIA et al, 2004;KAČÁNIOVÁ et al, 2012;LANA et al, 2005;MERESTA et al, 1989;SANCHEZ;GALARDI, 1988;SHALMANY;SHIVAZAD, 2006;TALAS et al, 2014;TATLI SEVEN et al, 2008;TEKELI et al, 2011). Dessa forma, a própolis apresenta-se como uma alternativa não-convencional para controlar processos infecciosos.…”