Antioxidant enzyme activities in liver tissue of chickens
fed diets supplemented with various forms
and amounts of selenium

Holovská, K.; Boldižárová, K.; Čekonová, S.; Lenártová, V.; Levkut, M.; Javorský, P.; Leng, Lubomir

doi:10.22358/jafs/67691/2003

Cited by 17 publications

(12 citation statements)

References 19 publications

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“…Chattopadhyay et al (2004) and Holovska et al (2003) observed increase in plasma antioxidant enzyme levels due to the supplementation of antioxidants in the diet of broilers. Rezaei-Moghadam et al (2012) has also reported that supplementation of turmeric increases serum antioxidant levels.…”

Section: Essential Oils In Meat Type Chickensmentioning

confidence: 99%

Essential Oils as a Feed Additive in Poultry Nutrition

Gopi¹

2014

Adv. Anim. Vet. Sci.

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Section: Essential Oils In Meat Type Chickensmentioning

confidence: 99%

Essential Oils as a Feed Additive in Poultry Nutrition

Gopi¹

2014

Adv. Anim. Vet. Sci.

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“…The effects of selenium sources on the performance of broiler chickens, carcass characteristics and meat quality were studied by Downs et al (2000), Ozkan et al (2007). The utilisation and availability of the microelement depends on its concentration and form (Holovská et al, 2003;Kuricová et al, 2003;Ševčíková et al, 2006).…”

mentioning

confidence: 99%

The effect of lycopene supplementation on lipid profile and meat quality of broiler chickens

Sevcikova¹,

Skřivan²,

Dlouhá³

2008

Czech J. Anim. Sci.

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Lycopene is a carotenoid that is mostly present in vegetables and in some fruit species as a red pigment (pineapple, orange, grapefruit, tomato, sweet pepper, strawberry). Tomatoes and products from tomatoes are the main source of lycopene while the tomato skin is a rich source of lycopene. Lycopene is extraordinarily efficient in the control of degenerative diseases, it is a preventive against cardiovascular diseases and cancer of prostate gland, digestive tract, skin, it decreases a risk of the cancer of pancreas, uterine uvula, and it blocks the formation of noxious cholesterol. Many studies dealing with the importance of lycopene for human health and disease have been published. Blum et al. (2006) found that tomato-rich diet increased the HDL-cholesterol level. Napolitano et al. (2007) investigated the effects of lycopene on the induction of foam cell formation by modified LDL. Their findings suggest that lycopene may reduce the macrophage foam cell formation induced by modified LDL by decreasing lipid synthesis and downregulating the activity and expression of scavenger receptor activity. There exist few papers dealing with the effect of lycopene in poultry diet. The effect of lycopene on performance and quality of meat and eggs in Japanese quail was studied by AbstrAct: An experiment was conducted to determine the effect of lycopene on lipid profile and quality of meat of broiler chickens Ross 308 at a different form of selenium. 540 broiler cockerels were randomly divided into 6 groups: without lycopene supplement (groups C and E3), supplemented with 50 mg/kg lycopene (groups E1 and E4), supplemented with 100 mg/kg lycopene (groups E2 and E5) while the source of selenium was sodium selenite (groups C, E1, E2) and Se-enriched yeast (groups E3, E4, E5). The experimental period was from 14 to 35 days of broiler age and was terminated by slaughter. The organic form of dietary selenium increased (P ≤ 0.05) its content in breast meat (E3 -174.2 μg/kg, E4 -186.4 μg/kg, E5 -191.9 μg/kg) compared to selenite (C -125.4 μg/kg, E1 -123.3 μg/kg, E2 -128.5 μg/kg). The shear force of meat was higher (P ≤ 0.05) in groups receiving the organic form of Se (E3 -0.026 kN, E4 -0.025 kN, E5 -0.024 kN) in comparison with group C (0.017 kN), E1 (0.016 kN) and E2 (0.014 kN). Se in Se-enriched yeast reduced (P ≤ 0.05) the concentration of malondialdehyde in breast meat after 5 days of storage. There were no significant differences in concentrations of cholesterol, LDL cholesterol and lipase in plasma. The higher content of HDL cholesterol in plasma was recorded in groups supplemented with 50 mg of lycopene, followed by groups with 100 mg of lycopene and the lowest values were measured in groups without lycopene supplementation when the difference between group E1 (1.64 mmol/l) and E3 (1.51 mmol/l) was significant (P ≤ 0.05). Concentrations of LDL cholesterol showed an opposite trend. The lycopene supplement had a positive effect on the lipid profile of blood plasma of broiler chickens.

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“…The enzymatic extract was obtained according to the same procedure described above, except from the fact that two different buffers where tested in the reaction medium: buffer 1, composed of potassium phosphate 50 mM at pH of activity has been measured in several types of meat, such as cattle DECKER, 1996aDECKER, , 1996bO'GRADY et al, 2001), pork (MAHAN;PARRETT, 1996;HERNÁNDEZ;PARK;RHEE, 2002), and poultry (MOREIRA et al, 2001;SURAI, 2002;CARRERAS et al, 2004, HOAC et al, 2006. GSH-Px catalyzes the detoxification of hydrogen peroxide (H 2 O 2 ) in vitro presenting high specificity for this substrate (ROTRUCK et al, 1973).…”

Section: Gsh-px Determination In Chicken Thigh After 120 Days Of Stormentioning

confidence: 99%

“…Holovská Junior et al (2003) studied the effect of four diets (without selenium, 0.2 mg of inorganic selenium.kg -1 , 0.2 mg of organic selenium.kg -1 , and 0.3 mg of organic selenium.kg -1 ) on chicken liver GSH-Px activity. They observed that changing the birds diet did not influence the chicken liver GSH-Px activity.…”

Section: Gsh-px Activity In Chicken Thigh Meat Six Hours After Slaughtermentioning

confidence: 99%

“…On the other hand, Moreira et al (2001) and Penha-Silva et al (2005) pre-incubated the medium at 37 °C for 10 minutes before adding the starter. Some authors report that the immediate reaction takes place at 37 °C, without pre-incubation (LINDMARK-MANSSON et al, 2001;HOLOVSKÁ JUNIOR et al, 2003;HOAC et al, 2006). Other studies report the use of temperatures for reactions that range from 20 °C to 25 °C (LEE; MEI; DECKER, 1997;HERNÁNDEZ;PARK;RHEE, 2002).…”

Section: Effect Of Different Pre-incubation Conditions Of the Reactiomentioning

confidence: 99%

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Investigation of glutathione peroxidase activity in chicken meat under different experimental conditions

et al. 2012

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Due to the fact that previous studies on the enzymatic activity of Glutathione peroxidase (GSH-Px) diverge widely in their methodology and results, this study aimed to investigate the influence of different analytical conditions on GSH-Px activity in chicken thighs from broilers that were fed different diets with different sources and concentrations of selenium. GSH-Px activity was evaluated six hours after slaughter and 120 days after frozen storage at -18 ºC. The different analytical conditions included time of pre-incubation (0, 10 and 30 minutes), reaction medium, types of substrate (H2O2 (0.72 mM, 7.2 mM, and 72 mM) and Terc-butil hydroperoxide 15 mM), and different buffer concentrations (buffer 1, potassium phosphate 50 mM pH 7.0 + EDTA 1 mM + mercaptoethanol 1 mM, and buffer 2, tris-HCl 50 mM pH 7.6 + EDTA 1 mM + mercapthanol 5 mM). The results show that the highest GSH-Px activity was observed when enzyme and substrate were in contact at 22 ºC without any pre-incubation, and that, when used at concentrations above 0.72 mM, hydrogen peroxide saturated the GSH-Px enzyme and inhibited its activity. The enzyme presented higher affinity to hydrogen peroxide when compared to terc-butil peroxide, and the addition of a buffer containing mercaptoethanol did not increase GSH-Px enzymatic activity. The activity of GSH-Px was not influenced by the source and concentration of selenium in the diet either. The obtained results allowed the determination of the best temperature of contact between the enzyme and substrate (22 ºC), the optimum concentration, and the type of substrate and buffer to be used. This information is extremely useful for future studies on GSH-Px activity in meat due to the divergence and little information found in the literature.

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Antioxidant enzyme activities in liver tissue of chickens fed diets supplemented with various forms and amounts of selenium

Cited by 17 publications

References 19 publications

Essential Oils as a Feed Additive in Poultry Nutrition

Essential Oils as a Feed Additive in Poultry Nutrition

The effect of lycopene supplementation on lipid profile and meat quality of broiler chickens

Investigation of glutathione peroxidase activity in chicken meat under different experimental conditions

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