Antioxidant enzyme activity and osmolyte content in winter cereal seedlings under hardening and cryostress

Kolupaev, Yu. Е.; Ryabchun, N. I.; Vayner, A. A.; Yastreb, Т. О.; Oboznyi, A. I.

doi:10.1134/s1021443715030115

Cited by 44 publications

(10 citation statements)

References 25 publications

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“…Thus, it has been shown that the basic resistance of rye seedlings to direct agents of oxidative stress (hydrogen peroxide and iron(II) sulfate) was significantly higher than that of bread wheat (Kolupaev et al, 2016). It is well known that rye is more frost resistant than most other cereals (Fu et al, 1998;Kolupaev et al, 2015). Fu et al (1998) associated rye resistance to both low and high temperatures with a significant accumulation of various carbohydrates, including fructans.…”

Section: Discussionmentioning

confidence: 99%

“…Evaluation of LPO products content. Analysis of the amount of lipid peroxidation (LPO) products reacting with 2-thiobarbituric acid (mainly malonic dialdehyde, MDA) in the shoots was carried out according to the protocol described earlier (Kolupaev et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

“…In this regard, attempts are being made to use markers of development of oxidative stress and state of antioxidant system to screen for heat-resistant cereal genotypes (Gupta et al, 2013). At the same time, strategies for plant adaptation to oxidative stress depend on their taxonomic affiliation (Kolupaev et al, 2015;2016;. In this regard, a comparison of the functioning of stress-protective systems in cereals belonging to different genera and species and having different resistance is of interest both for a deeper understanding of specific characteristics of adaptation and for developing methods for assessing resistance of varieties and lines that can be used in breeding.…”

Section: Introductionmentioning

confidence: 99%

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Adaptation of Cereal Seedlings to Oxidative Stress Induced by Hyperthermia

KOLUPAEV¹,

MAKAOVA²,

Ryabchun³

et al. 2022

AgricultForest

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The response of etiolated seedlings of winter rye (Secale cereale), bread (Triticum aestivum) and durum (T. durum) wheats, as well as triticale (× Triticosecale) to the action of hyperthermia in relation to their resistance to oxidative stress was studied. Exposure of seedlings to 45°C for 4 hours led to a significant inhibition of the growth of T. aestivum, while the growth of T. durum and × Triticosecale seedlings was less sensitive to hyperthermia, and S. cereale seedlings showed the greatest resistance to heat stress. In bread wheat seedlings after heating, intensive development of oxidative stress. In durum wheat and triticale, such effects were less pronounced, and in rye, they were almost absent. In rye, triticale, and durum wheat seedlings, peroxidase activity increased under hyperthermia conditions, while in bread wheat, on the contrary, it decreased. In all four studied cereals, in response to the action of high temperature, the content of multifunctional stress metabolite proline increased, however, in rye, its absolute content significantly exceeded that in other species. The content of sugars during hyperthermia increased in S. cereale and T. durum, but did not change in the other two cereals. Triticale and especially rye have a high base content of anthocyanins and its increase in response to high temperature. A conclusion was made about the relationship between the ability of cereal seedlings to maintain growth under the action of hyperthermia and their resistance to oxidative stress, which is mainly due to the accumulation of metabolites with antioxidant activity.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Adaptation of Cereal Seedlings to Oxidative Stress Induced by Hyperthermia

KOLUPAEV¹,

MAKAOVA²,

Ryabchun³

et al. 2022

AgricultForest

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“…It is known that the hydrolysis of starch is enhanced when the temperature drops to −3°С or −5°С. This leads to the accumulation of sugars, which is an adaptation to hypothermia in winter (Tumanov 1976;Kolupaev and Trunova 1992;Kolupaev et al 2015;Zeng et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Dynamics of non-structural carbohydrates inPinus pallasianaD. Don needles under different forest growth conditions of ravine anti-erosion plantations

Bessonova

Yakovlieva-Nosar²

2022

Folia Forestalia Polonica

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The research was aimed at analysing the peculiarities of non-structural carbohydrate metabolism in the needles of Pinus pallasiana D. Don in anti-erosion plantations on the slope and in the thalweg of the Viyskovyi ravine under different forest growth conditions. The ravine is located in the Dnipropetrovsk region and belongs to the southern geographical variant of ravine forests. The studied plants grew at three experimental sites of man-made plantation: in the thalweg (forest growth conditions – mesophilic, fresh, CL2), in the middle part of the slope of the southern exposure (mesoxerophilic, somewhat dry, or semi-arid, CL1) and on the upper part of this slope (xerophilic, arid, CL0–1). Changes in glucose, fructose, reducing sugars, sucrose and starch during the period from May to October were studied. The concentration of osmotically active substances (glucose, sucrose) increases in the months with the most unfavourable hydrothermal parameters, especially in plants of mesoxerophilic and xerophilic forest growth conditions. The dynamics of fructose content revealed two maxima (July and October), when the concentration of glucose tends to increase steadily, with the highest values found in October. During the study period, the level of fructose in the needles under the arid conditions of the upper and middle parts of the slope was much lower than under the fresh conditions of the thalweg. The dynamics curve of the starch content tends to decrease from the maximum values in May to the first minimum in August. It is followed by some increase in the concentration of this polysaccharide that does not reach the previous values. The second minimum is registered in October. Such changes in the level of starch in the needles of plants are observed under all forest growth conditions. The decrease in polysaccharide content is consistent with the increase in glucose and sucrose, especially in August. A more significant increase in the concentration of soluble osmoprotective sugars (glucose, sucrose) in P. pallasiana needles under unfavourable hydrothermal conditions compared to normal moisture conditions indicates the ability of plants to adapt to mesoxerophilic and xerophilic conditions.

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“…Furthermore, higher activities of CAT and GPX, especially at the advanced time-points of CA, seem to be involved in this phenomenon. Low temperature was proved to increase the activity of SOD and CAT in Hordeum vulgare , S. cereale, Triticum aestivum, and T. durum [ 73 ]. Furthermore, in the other research on T. aestivum it was shown that low temperature increased the activity of SOD, APX, GR, and POD [ 74 ].…”

Section: Discussionmentioning

confidence: 99%

Freezing Tolerance of Lolium multiflorum/Festuca arundinacea Introgression Forms is Associated with the High Activity of Antioxidant System and Adjustment of Photosynthetic Activity under Cold Acclimation

Augustyniak

Pawłowicz

Lechowicz

et al. 2020

IJMS

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Though winter-hardiness is a complex trait, freezing tolerance was proved to be its main component. Species from temperate regions acquire tolerance to freezing in a process of cold acclimation, which is associated with the exposure of plants to low but non-freezing temperatures. However, mechanisms of cold acclimation in Lolium-Festuca grasses, important for forage production in Europe, have not been fully recognized. Thus, two L. multiflorum/F. arundinacea introgression forms with distinct freezing tolerance were used herein as models in the comprehensive research to dissect these mechanisms in that group of plants. The work was focused on: (i) analysis of cellular membranes’ integrity; (ii) analysis of plant photosynthetic capacity (chlorophyll fluorescence; gas exchange; gene expression, protein accumulation, and activity of selected enzymes of the Calvin cycle); (iii) analysis of plant antioxidant capacity (reactive oxygen species generation; gene expression, protein accumulation, and activity of selected enzymes); and (iv) analysis of Cor14b accumulation, under cold acclimation. The more freezing tolerant introgression form revealed a higher integrity of membranes, an ability to cold acclimate its photosynthetic apparatus and higher water use efficiency after three weeks of cold acclimation, as well as a higher capacity of the antioxidant system and a lower content of reactive oxygen species in low temperature.

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Antioxidant enzyme activity and osmolyte content in winter cereal seedlings under hardening and cryostress

Cited by 44 publications

References 25 publications

Adaptation of Cereal Seedlings to Oxidative Stress Induced by Hyperthermia

Adaptation of Cereal Seedlings to Oxidative Stress Induced by Hyperthermia

Dynamics of non-structural carbohydrates inPinus pallasianaD. Don needles under different forest growth conditions of ravine anti-erosion plantations

Freezing Tolerance of Lolium multiflorum/Festuca arundinacea Introgression Forms is Associated with the High Activity of Antioxidant System and Adjustment of Photosynthetic Activity under Cold Acclimation

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