Antioxidant enzymes activity and physiological response of tomato (Lycopersicon esculentum M.) genotypes under mild temperature stress

Abstract

Tomato being sensitive to high temperature experiences mild to high temperature stresses under climate change conditions. To understand the response of tomato genotypes to mild temperature stress, a study was conducted in temperature gradient tunnel facility. The results revealed that across the genotypes studied, specific activity of antioxidant enzymes viz., superoxide dismutase (SOD), peroxidase (POX) and glutathione reductase (GR) increased significantly. Among the genotypes, increase in SOD activity was highest in cv. Arka Vikas, followed by IIHR 2195 and least in Abhinava. The GR activity was highest in Abhinava, followed by IIHR 2195 and least in cv. Arka Vikas. The mild temperature stress caused reduction in catalase (CAT) activity. The decrease in CAT activity and concomitant increase in POX activity was observed in cv. Arka Vikas. Low leaf water potential (W leaf ) and higher electrolyte leakage indicated that the membrane integrity was affected across the tomato genotypes even under mild temperature stress. Among the genotypes studied, cv. Arka Vikas showed greater activity of SOD and POX, higher membrane stability and least reduction in water potential under mild temperature stress.

Particulate Nanoscale Silica Induced Novel Morphological and Biochemical Stimulus Effects in Chilli (Capsicum annuum L.)

“…Peroxidase activity was estimated using o -phenyl diamine (OPD) . In brief, 0.2 g of leaf was thoroughly ground with 5 mL of 50 μM citrate buffer (pH 6.4).…”

Induction Temperature Enhances Antioxidant Enzyme Activity and Osmoprotectants in Tomato

“…However highest increase in activity of SOD over control was recorded in genotypes Arka Rakshak (964.9%) followed by IIHR-329 (380.3%) and least increase in enzyme activity was observed in genotypes Arka Abha and CO-3. Enhanced activity of antioxidant enzymes may increases high temperature stress tolerance by detoxification of the heavy load of ROS, which in turn provides protection through homeostasis in plants (Sairam and Tyagi, 2004;Laxman et al, 2014;Revero et al, 2004). Increased SOD activity was observed in tolerant tomato genotypes compared to sensitive genotypes (Laxman et al, 2014).…”

“…The mechanism includes production of antioxidant enzymes such as superoxide dismutase (SOD; EC 1.15.1.1), peroxidase (POD; EC 1.11.1.7), catalase (CAT; EC 1.11.1.6) and glutathione reductase (GR; EC 1.8.1.7) and non enzymatic antioxidants such as Carotenoids, flavonoids and ascorbic acids. Tolerance to high temperature stress in crop plants has been associated with an increase in antioxidative capacity (Camejo et al, 2002;Laxman et al, 2014). Hence antioxidant enzymes play a very important role as a defence mechanism for protecting cellular membranes and organelles against ROS (Rivero et al, 2004).…”

Antioxidant Protection of Photosynthesis in Two Cashew Progenies Under Salt Stress

“…However, the activity of this enzyme in the shoots was 35% higher on average in CCP09 than in CCP76 at all doses. POX has been associated with antioxidant protection against diverse abiotic stresses (Abdelgawad, Khalafaallah, & Abdallah, 2014;Laxman et al, 2014) and the control of growth under various conditions of stress (Maia et al, 2012). In the present work, the significant increase in POX activity may be related, at least in part, to the decrease in accumulation of biomass in cashew tree shoots, given the early maturation of tissues caused by increased POX activity.…”