Antioxidant enzymes in the honey bee, 
<i>Apis mellifera</i>

Weirich, Günter F.; Collins, Anita M.; Williams, Virginia P.

doi:10.1051/apido:2001001

Cited by 165 publications

(133 citation statements)

References 38 publications

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“…The values of TAC measurements in whole-body homogenate or segments are limited because the specific origin of antioxidant protection cannot be defined. We demonstrated TAC in haemolymph for the first time, extending our knowledge of total antioxidative protection in honeybee haemolymph (Weirich et al 2002).…”

Section: Discussionsupporting

confidence: 59%

“…To date, activity of SOD, CAT and GST has been found in honeybee haemolymph (Weirich et al 2002). Activity of antioxidant enzymes is low and the honeybee genome is characterised by low number of genes coding for antioxidative protein (Corona and Robinson 2006), which contributes to limited defence against ROS in comparison to other insects.…”

Section: Discussionmentioning

confidence: 99%

“…The most important antioxidant enzymes identified in honeybee body fluids are superoxide dismutase (SOD), catalase (CAT), glutathione Stransferase (GST) and peroxidase (Weirich et al 2002;Collins et al 2004Collins et al , 2006. To date, 39 genes coding ten groups of antioxidant proteins have been identified in the honeybee genome (Corona and Robinson 2006).…”

Section: Introductionmentioning

confidence: 99%

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Total antioxidant capacity of honeybee haemolymph in relation to age and exposure to pesticide, and comparison to antioxidant capacity of seminal plasma

et al. 2015

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-Oxidative stress is defined as a disturbance in the balance between the production of reactive oxygen species and antioxidant defences. We measured total antioxidant capacity (TAC) in honeybee haemolymph and seminal plasma and analysed TAC of haemolymph in relation to age and exposure to pesticide. TAC of haemolymph increased with age of bees (1.18 vs 1.97 mM of (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) for 1-and 30-day-old bees, respectively, P ≤0.05). Exposure to imidacloprid (IMD) affected TAC of haemolymph of 1-day-old but not 30-day-old honeybees. TAC in haemolymph of 1-day-old bees was lower in treatments with the addition of 5 and 200 ppb IMD (1.57-1.46 mM of Trolox in treated bees compared with 2.37 mM of Trolox in controls; P ≤0.05). In conclusion, antioxidant protection of honeybees seems to be related to age and may be disturbed by exposure to IMD. Older bees with higher antioxidant protection seem to be less susceptible to IMD toxicity. The toxic effect of pesticide seems to be particularly dangerous in early life stages of honeybees.Apis mellifera / total antioxidant capacity / haemolymph / ageing / pesticide

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Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Total antioxidant capacity of honeybee haemolymph in relation to age and exposure to pesticide, and comparison to antioxidant capacity of seminal plasma

et al. 2015

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“…In addition, they live in large colonies, are easily manipulated, and their genome has been sequenced (Remolina et al 2007;Neukirch 1982;Rueppell et al 2007a, b). A variety of honeybee specimens, including spermathecae, muscle, ventriculi, hemolymph plasma, semen, brain, trophocytes, and fat cells, have been used to study oxidative stress and aging (Weirich et al 2002;Seehuus et al 2006a, b;Williams et al 2008;Collins et al 2004;Corona et al 2005;Hsieh and Hsu 2011a, b).…”

Section: Introductionmentioning

confidence: 99%

Changes in mitochondrial energy utilization in young and old worker honeybees (Apis mellifera)

Chuang

Hsu

2012

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Trophocytes and fat cells in honeybees (Apis mellifera) have served as targets for cellular senescence studies, but mitochondrial energy utilization with advancing age in workers is unknown. In this study, mitochondrial energy utilization was evaluated in the trophocytes and fat cells of young and old workers reared in a field hive. The results showed that (1) mitochondrial density increased with advancing age; (2) mitochondrial membrane potential (ΔΨm), nicotinamide adenine dinucleotide oxidized form (NAD + ) concentration, adenosine triphosphate (ATP) concentration, and NAD + /nicotinamide adenine dinucleotide reduced form (NADH) ratio decreased with advancing age; and (3) the expression of NADH dehydrogenase 1 (ND1), ATP synthase, and voltage-dependent anion channel 1 (VDAC1) increased with advancing age, whereas ND1 and ATP synthase did not differ with advancing age after normalization to mitochondrial density and VDAC1. These results show that the trophocytes and fat cells of young workers have higher mitochondrial energy utilization efficiency than those of old workers and that aging results in a decline in mitochondrial energy utilization in the trophocytes and fat cells of worker honeybees.

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“…Therefore, honeybees have been the model system for many aging studies (Remolina et al 2007;Neukirch 1982;Rueppell et al 2007a, b). A variety of honeybee specimens, including spermathecae, muscle, ventriculi, hemolymph plasma, semen, brain, trophocytes, and fat cells, have been used to study oxidative stress (Weirich et al 2002;Seehuus et al 2006a, b;Williams et al 2008;Collins et al 2004;Corona et al 2005) and aging (Hsieh and Hsu 2011).…”

Section: Introductionmentioning

confidence: 99%

The use of honeybees reared in a thermostatic chamber for aging studies

Hsu

Chan

2011

AGE

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Honeybees (Apis mellifera) are an attractive model system for studying aging. However, the aging level of worker honeybees from the field hive is in dispute. To eliminate the influence of task performance and confirm the relationship between chronological age and aging, we reared newly emerged workers in a thermostat at 34°C throughout their lives. A survivorship curve was obtained, indicating that workers can be reared away from the field hive, and the only difference between these workers is age. To confirm that these workers can be used for aging studies, we assayed age-related molecules in the trophocytes and fat cells of young and old workers. Old workers expressed more senescence-associated β-galactosidase, lipofuscin granules, lipid peroxidation, and protein oxidation than young workers. Furthermore, cellular energy metabolism molecules were also assayed. Old workers exhibited less ATP concentration, β-oxidation, and microtubule-associated protein light chain 3 (LC3) than young workers. These results demonstrate that honeybees reared in a thermostatic chamber can be used for aging studies and cellular energy metabolism in the trophocytes and fat cells of workers changes with advancing age.

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Antioxidant enzymes in the honey bee, Apis mellifera

Cited by 165 publications

References 38 publications

Total antioxidant capacity of honeybee haemolymph in relation to age and exposure to pesticide, and comparison to antioxidant capacity of seminal plasma

Total antioxidant capacity of honeybee haemolymph in relation to age and exposure to pesticide, and comparison to antioxidant capacity of seminal plasma

Changes in mitochondrial energy utilization in young and old worker honeybees (Apis mellifera)

The use of honeybees reared in a thermostatic chamber for aging studies

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