Antioxidant, free radical scavenging and invitro cytotoxic studies of ethanolic extract of Leucas indica var lavandulifolia and Leucas indica var nagalapuramiana

Ramalingam, R. S.; Sudini, Sushruthi; Boddupalli, Bindu Madhavi; Anisetti, Ravinder Nath

doi:10.1016/s2221-1691(12)60468-7

Cited by 11 publications

(6 citation statements)

References 14 publications

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“…Our data suggest that the methanolic extract is more potent than the aqueous extract in scavenging hydroxyl radicals (Figure 2c). Similar antioxidative potency has been noted in other plants, including Leucas indica, 54 Phyllanthus sacidus 55 and Azima tetracantha. 56 Previous reports point to positive associations between supplementation with antioxidative compounds and decreased frequency of occurrence and progression of senile cataracts in human 57 and in selenite cataracts in experimental animals.…”

Section: Discussionsupporting

confidence: 79%

A Methanolic Extract of Ocimum basilicum Exhibits Antioxidant Effects and Prevents Selenite-induced Cataract Formation in Cultured Lenses of Wistar Rats

Anand¹,

Sundararajan²,

Anbukkarasi³

et al. 2019

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Objective: An extract (Methanolic) of the Ocimum basilicum leaf was analysed for potential to abrogate experimental formation of cataract in-vitro. Methods: Phytoconstituents were first detected in O. basilicum extracts (Aqueous or methanolic) by gas chromatographic-mass spectrometric analysis. The putative antioxidant activity of these extracts was then assessed by measuring in-vitro radical-scavenging activity, ion-chelating potential and reducing potency. Potential cytotoxicity of the extract on Human lenticular epithelial B3 (HLE-B3) cells was also sought. Finally, possible prevention of cataract formation by the methanolic extract was gauged in selenite-exposed lenses obtained from Wistar rats. There were 3 groups (8 Lenses in each): Group I (Lenses incubated in Dulbecco's modified Eagle's medium [DMEM] alone); Group II (Lenses incubated in DMEM with sodium selenite [100 µM/ml]); Group III (Lenses incubated in DMEM with selenite [100 µM/ml] and the O. basilicum methanolic extract (200 µg/ml DMEM). Gross lenticular morphology was assessed. Levels of lenticular malondialdehyde (MDA) and reduced glutathione (GSH) were also measured. Results: A higher intensity of antioxidative activity was noted in the methanolic extract than in the aqueous extract. The methanolic extract exhibited negligible cytotoxicity. On morphological examination, marked opacification was seen in all 8 Group II lenses whereas there was no opacification in 7 of 8 Group III lenses. Near normal mean levels of reduced glutathione and malondialdehyde, were noted within Group III lenses. Conclusion: The methanolic extract of the O. basilicum leaf appears to prevent selenite-induced cataract formation in-vitro.

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Section: Discussionsupporting

confidence: 79%

A Methanolic Extract of Ocimum basilicum Exhibits Antioxidant Effects and Prevents Selenite-induced Cataract Formation in Cultured Lenses of Wistar Rats

Anand¹,

Sundararajan²,

Anbukkarasi³

et al. 2019

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“…Hydrogen peroxide is a weak oxidant produced during metabolic processes and can produce hydroxyl radicals in the cell, which will lead to cell damages. The addition of antioxidants to H 2 O 2 solution causes fast decrease in the absorbance at 230 nm (Ramani et al, 2012). In the present study, the crude sample (ESPS) showed a noticeable capability to scavenge.…”

Section: Discussionmentioning

confidence: 45%

Antioxidant and antimitotic activities of sulfated polysaccharide from marine brown algae Padina tetrastromatica

Jose¹,

Radhakrishnan²,

Kurup³

2015

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Antioxidants play a central role in the prevention of carcinogenesis. The most natural compounds exhibit their protective effects by eliciting antioxidant potential. Sulfated polysaccharide was isolated from the brown algae Padina tetrastromatica, then purified and evaluated for its composition and in vitro antioxidant and antimitotic activities. Both ethanolic sulfated polysaccharide (ESPS) and ethanolic sulfated polysaccharide-column purified (ESPS-CP) exhibited considerable amount of carbohydrates (11.2% and 17.6%), sulfate (11.4% and 7.4%), fucose (5.5% and 15.7%), uronic acid (4.7% and 11.8%), xylose (0.5% and 0.03%) and sulfated polysaccharide (2.4% and 12.7%) content. The FTIR analysis and phytochemical screening also confirmed the presence of sulfated polysaccharides. In the in vitro antioxidant activity determination using DPPH (1-1-diphenyl 2-picryl hydrazyl) radical scavenging activity, hydroxyl radical scavenging activity, superoxide anion scavenging activity, hydrogen peroxide scavenging activity, total antioxidant activity and reducing power, ESPS showed more activity than ESPS-CP. In the case of nitric oxide radical scavenging, ESPS-CP was found to be more effective. At a concentration of 2mg/ml, both samples were potent antioxidants with significant IC50 values. The antimitotic studies such as mitotic index in onion root tips and sprouting assay in green gram seeds also proved that both the extracts are able to prevent mitosis. The extrapolation of these results can find opportunities in therapeutic regiments of cancer.

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“…The extract at high concentration was shown to decrease the amount of nitric oxide production within the test system. Scavengers of nitric oxide compete with oxygen leading to a reduced production of nitrite ions [ 18 , 19 ]. Indeed, studies abound that suggest the role of relatively low concentrations of nitric oxide in favouring cell proliferation and antiapoptotic responses while higher levels are considered to favour pathways that induce cell-cycle arrest, mitochondria respiration, senescence, or apoptosis [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

Free Radical Scavenging Activity: Antiproliferative and Proteomics Analyses of the Differential Expression of Apoptotic Proteins in MCF-7 Cells Treated with Acetone Leaf Extract ofDiospyros lycioides(Ebenaceae)

Pilane

Bagla

Mokgotho

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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Breast cancer is the most common cancer in South Africa. The acetone leaf extract of Diospyros lycioides was evaluated qualitatively and quantitatively for its antioxidant potential using DPPH assay and nitric oxide radical scavenging effect, while the viability of MCF-7 cells was evaluated using the MTT. MCF-7 treated cells were stained with Hoechst 335258 dye and annexin-V-FITC to be evaluated for apoptotic effect of the extract, while mRNA expression levels of apoptotic genes were assessed by quantitative real-time PCR and deferential protein expression levels using 2D gel electrophoresis and mass spectrometry. Results revealed presence of antioxidant constituents in the extract. Extract was shown to be cytotoxic in a concentration- and time-dependent manner. Cytotoxicity was demonstrated to be due to apoptosis, with 70% of the extract-treated cells being annexin-V-positive/PI negative at 48 hours. The extract was also shown to upregulate the expression of p53 gene with concomitant downregulation of the Bcl-2 antiapoptotic gene while differentially expressed proteins were identified as enolase, pyruvate kinase, and glyceraldehyde-3-phosphate. The extract in this study was shown to induce apoptosis at an early stage which makes it an ideal source that can be explored for compounds that may be used in the treatment and management of cancer.

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Antioxidant, free radical scavenging and invitro cytotoxic studies of ethanolic extract of Leucas indica var lavandulifolia and Leucas indica var nagalapuramiana

Cited by 11 publications

References 14 publications

A Methanolic Extract of Ocimum basilicum Exhibits Antioxidant Effects and Prevents Selenite-induced Cataract Formation in Cultured Lenses of Wistar Rats

A Methanolic Extract of Ocimum basilicum Exhibits Antioxidant Effects and Prevents Selenite-induced Cataract Formation in Cultured Lenses of Wistar Rats

Antioxidant and antimitotic activities of sulfated polysaccharide from marine brown algae Padina tetrastromatica

Free Radical Scavenging Activity: Antiproliferative and Proteomics Analyses of the Differential Expression of Apoptotic Proteins in MCF-7 Cells Treated with Acetone Leaf Extract ofDiospyros lycioides(Ebenaceae)

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