Antioxidant, Gastroprotective, Cytotoxic Activities and UHPLC PDA-Q Orbitrap Mass Spectrometry Identification of Metabolites in Baccharis grisebachii Decoction

Abstract

The decoction of the local plant Baccharis grisebachii is used as a digestive, gastroprotective, external cicatrizing agent and antiseptic in Argentine. A lyophilized decoction (BLD) from the aerial parts of this plant was evaluated regarding its anti-ulcer, antioxidant and cytotoxic activities and the bioactivities were supported by UHPLC-MS metabolome fingerprinting which revealed the presence of several small bioactive compounds. The antioxidant properties were evaluated by DPPH, TEAC, FRAP and lipoperoxidation inhibition in erythrocytes methods, and the antibacterial activity was evaluated according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. The BLD showed a moderate free radical scavenging activity in the DPPH (EC50 = 106 µg/mL) and lipid peroxidation in erythrocytes assays (67%, at 250 µg/mL). However, the BLD had the highest gastroprotective effect at a dose of 750 mg/kg with a ninety-three percent inhibition of damage through a mechanism that involve NO and prostaglandins using the ethanol-induced gastric damage in a standard rat model. On the other hand, BLD does not induce cytotoxic changes on human tumor and no-tumor cell lines at the concentrations assayed. Regarding the metabolomic analysis, thirty-one compounds were detected and 30 identified based on UHPLC-OT-MS including twelve flavonoids, eleven cinnamic acid derivatives, one coumarin, one stilbene and two other different phenolic compounds. The results support that the medicinal decoction of Baccharis grisebachii is a valuable natural product with gastroprotective effects and with potential to improve human health that opens a pathway for the development of important phytomedicine products.

UHPLC-HESI-OT-MS-MS Biomolecules Profiling, Antioxidant and Antibacterial Activity of the “Orange-Yellow Resin” from Zuccagnia punctata Cav.

“…The orange-yellow resin from Z.punctata (ZpRe) displayed a stronger DPPH scavenging activity with an EIC 50 25.72 µg/mL, as well as an outstanding inhibition of lipid peroxidation in erythrocytes (70% percent at 100 µg ZpRe/mL), this was comparable to the value shown by the reference compound catechin (74% at 100µg/mL) ( Table 2) and to the values shown recently by others Andean species [14,15,18]. Phenolic antioxidant compounds acting as free radical scavengers can delay or inhibit lipid oxidation processes.…”

“…The ability of the ZpReresin at three concentrations (100, 250, and 500 µg/mL) and of catechin at a single concentration (100 µg/mL) to inhibit lipoperoxidation in erythrocytes (LP), induced by tert-Butyl hydroperoxide, was determined. Relevant technical aspects of the trial have been reported recently in detail [15,18]. The values obtained are expressed as percentages of lipid oxidation inhibition.…”

Rhaponticin as an anti-inflammatory component of rhubarb: a minireview of the current state of the art and prospects for future research

“…Rhaponticin was also detected in other plants such as Trigonella foenum-graecum L. (fenugreek) (He et al 2016;Li et al 2018), Guibourtia tessmannii (Harms) J. Leonard (Nyemba et al 1995), Stuhlmannia moavi Taub. (Liu et al 2013), Eucalyptus rubida H. Deane & Maiden (Etoh et al 1990) and Baccharis grisebachii Hieron (Gómez et al 2019).…”

Two New Flavonoids from the Leaves of Baccharis oblongifolia (Ruiz and Pav.) Pers. (Asteraceae)

“…Some Baccharis species are used in folk medicine, including for spasmolytic, diuretic, and analgesic purposes and for the treatment of ulcers, fever, gastrointestinal illnesses, diabetes, and microbial infections [4,5,6]. Previous phytochemical studies of this genus have reported several classes of natural products, such as chlorogenic acid derivatives [4,7,8,9,10], terpenoids [11,12,13,14,15,16,17,18,19,20,21,22], especially diterpenes with dozens of isolated compounds from Baccharis [4,5,17,18,19,20,21,22], and flavonoids from flavone, flavanone, and flavonol types [4,5,21,22,23,24,25,26]. Furthermore, it has also been described as having diverse biological activities including antimicrobial [4,5,6,14,15], antiviral [4,5,6], antiprotozoal [9,24,25], anti-inflammatory [10,13], cytotoxic [4,5,6,23], and antioxidant [7,13,14,15,22,…”