Antioxidant Mechanism of Lactiplantibacillus plantarum KM1 Under H2O2 Stress by Proteomics Analysis

Abstract

Lactiplantibacillus plantarum KM1 was screened from natural fermented products, which had probiotic properties and antioxidant function. The survival rate of L. plantarum KM1 was 78.26% at 5 mM H2O2. In this study, the antioxidant mechanism of L. plantarum KM1 was deeply analyzed by using the proteomics method. The results demonstrated that a total of 112 differentially expressed proteins (DEPs) were screened, of which, 31 DEPs were upregulated and 81 were downregulated. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated that DEPs participated in various metabolic pathways such as pyruvate metabolism, carbon metabolism, trichloroacetic acid cycle, amino acid metabolism, and microbial metabolism in diverse environments. These metabolic pathways were related to oxidative stress caused by H2O2 in L. plantarum KM1. Therefore, the antioxidant mechanism of L. plantarum KM1 under H2O2 stress provided a theoretical basis for its use as a potential natural antioxidant.

Lactiplantibacillus plantarum and Saussurea costus as Therapeutic Agents against a Diabetic Rat Model—Approaches to Investigate Pharmacophore Modeling of Human IkB Kinase and Molecular Interaction with Dehydrocostus Lactone of Saussurea costus

“…Importantly, the TGs buildup in hepatocytes was previously linked with insulin resistance, putting the liver at risk of additional damage [ 57 ]. Additionally, alloxan induces the liver’s conversion of fatty acids to cholesterol and increases triglyceride formation, released into the bloodstream as lipoproteins [ 58 ]. Hence, the liver’s stellate cells may be stimulated by oxidative stress and steatohepatitis, which are detected in this study, leading to fibrosis.…”

Probiotic assessment and antioxidant characterization of Lactobacillus plantarum GXL94 isolated from fermented chili

“…In recent years, some researchers have used omics studies to reveal the molecular mechanism of the antioxidant performance of L. plantarum. The results showed that various metabolic pathways such as base excision repair system, recombinational DNA repair pathway, pyruvate metabolism, carbon metabolism, trichloroacetic acid cycle, amino acid metabolism, and microbial metabolism were closely related to these antioxidant properties ( Zhai et al, 2020 ; Tian et al, 2022 ). In future studies, we can use these methods to further explore the molecular mechanism of GXL94 antioxidant properties.…”