Antioxidant Mediated Defense Role of Ecliptaalba Herbal Extract Against Ccl4 Inducedtoxic Hepatitis

Abstract

Objective: The present study focuses on the antioxidant mediated the protective effect of a popular medicinal herb Ecliptaalba against CCl4 induced oxidative stress in the liver of rats.Methods: Rats divided into four groups were administered with CCl4 andCCl4 along with methanol extract of leaves of Eclipta herb (200 and 400 mg/kg b. wt) for three weeks. At the end of treatment, rats were anaesthetized and blood samples were collected for serum separation. Biochemical analysis such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and total bilirubin (TB) was done in serum. Liver tissue was used for glutathione (GSH), lipid peroxidation (LPO), glutathione peroxidase (GPx), glutathione S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) analysis. CCl4 administration caused significant elevation of the serum enzymes and total bilirubin content.Results: Antioxidant enzymes were drastically inhibited with a significant reduction of glutathione and increased lipid peroxidation in CCl4 treated rats. Ecliptaalba extracts administrated rats, however, did not show much changes in marker enzyme level. Antioxidant enzyme status was also not affected and found nearer to the control levels. Increased gluatathione level and reduced lipid peroxidation were also evident in Eclipta alba treated rats. Conclusion:The findings show that Eclipta alba extract offers better protection against the free radical toxicity of CCl4.

Assessment of the Potential Role of L- Methionine on Nickel Sulfate Induced Renal Injury and Oxidative Stress in Rat

“…Through the absence of enough GPx activity or GSH levels, LOP and hydrogen peroxide are not detoxified and perhaps converted by transition metals to hydroxyl radicals and lipid peroxyl radicals. The findings were similar to the results of Kubrak et al [36] and Dahmen-Ben Moussa et al [21] who found decreased GPx, CAT and SOD activities in the rat kidney by Ni cytotoxicty of antioxidant capacity may be coupled with the depletion of GSH and reduced total sulfhydryl contents or with their increased utilization for quenching free radicals and/or their inactivation by excessive Ni oxidation. Furthermore, Ni was reported to trigger nitric oxide (NO) creation [43], which may inhibit both CAT activities by binding to its heme prosthetic group [44] and GPx activity [45].…”

“…Therefore they can protect the body from oxidative stress and degenerative diseases. GSH is an essential endogenous antioxidant, acts as a cofactor for antioxidant enzymes, directly scavenging ROS and protects cells against free radicals and toxic compounds [36]. The results of nickel exposure were coincide with the findings of Amudha and Pari [2] and Boulila et al [5].…”

Nephroprotective Activity of the Ethnomedicinal Plants Caesalpinia Bonduc and Momordica Dioica From Ne India Against Cisplatin Induced Chemotherapeutic Toxicity

“…Secondary metabolites such as terpenoids that gives plants their odors and others metabolites (quinines and tannins) are responsible for plant flavor and some of the herbs and spices used by humans to season food yield useful medicinal compounds. The phenolic and flavonoid compounds are known as powerful chain-breaking antioxidants for their scavenging ability and which is due to their hydroxyl groups [17][18][19][20][21][22][23][24][25]. The phenolic and flavonoids compounds are the most important groups of plant secondary metabolites.…”

N-Acetylcysteine, a Boon for Yellow Phosphorus-Induced Acute Liver Failure? A Case Report

“…It acts as a scavenger of oxygen free radicals and it replenishes the glutathione stores. It is found to have a good prognosis in patients with yellow phosphorus poisoning when administered early [13,14]. The patient's liver enzymes were highly elevated.…”