Antioxidant‐modulated cytotoxicity of silver nanoparticles

Barbasz, Anna; Oćwieja, Magdalena; Piergies, Natalia; Duraczyńska, Dorota; Nowak, A.

doi:10.1002/jat.4173

Cited by 13 publications

(6 citation statements)

References 56 publications

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“…Previously considered results (Barbasz et al, 2021) proved that GA stabilizes GAAgNPs in two forms, namely, as hydroxylated aliphatic and phenolic moieties. Such a statement was based on the appearance of the strong intensity bands at 2927 cm −1 assigned to the aliphatic C–H stretching mode (ν(CH)) and at the same time at 1592‐cm −1 characteristic for the aromatic C═C stretching vibrations (ν(C═C) ϕ ) in the SERS spectrum of GAAgNPs (Barbasz et al, 2021). As it was mentioned above, such possible coexistence of two forms of GA on the AgNPs has been already proved (Alvarez‐Ros et al, 2001, 2003).…”

Section: Resultsmentioning

confidence: 97%

Design cytotoxicity: The effect of silver nanoparticles stabilized by selected antioxidants on melanoma cells

Barbasz

Czyżowska

Piergies

et al. 2021

J of Applied Toxicology

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Silver nanoparticles (AgNPs) prepared and stabilized by diverse biologically active substances seem to be especially useful in diverse biological and medical applications. The combination of AgNPs with bioactive substances, such as antioxidants, can lead to the development of new systems of desired anticancer properties. In this research, AgNPs were prepared with the use of diverse antioxidant combinations including gallic acid (GA), (−)‐epicatechin‐3‐gallate (EGCG), and caffeine (CAF). The insightful physicochemical characteristic revealed that each type of AgNPs exhibited spherical shape, comparable size distribution and negative surface charge. Surface‐enhanced Raman spectroscopy (SERS) delivered the information about the chemistry of AgNP stabilizing layers, which turned out to be a crucial factor tuning toxicity of AgNPs toward murine B16 melanoma cells (B16‐F0) and human skin melanoma (COLO 679) cells. EGCGAgNPs were the most cytotoxic among all the investigated AgNPs. They strongly reduced the activity of mitochondria, damaged cell membrane integrity, and penetrated inside the cells causing DNA damage. In turn, the toxicity of GAAgNPs strongly manifested via the induction of oxidative stress in the cells. It was found that CAFGAAgNPs exhibited the lowest toxicity toward the melanoma cells, which proved that a proper combination of antioxidants enable to prepare AgNPs of differentiated toxicity. It was established that human skin melanoma cells were significantly more sensitive to AgNPs than the murine melanoma cells.

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Section: Resultsmentioning

confidence: 97%

Design cytotoxicity: The effect of silver nanoparticles stabilized by selected antioxidants on melanoma cells

Barbasz

Czyżowska

Piergies

et al. 2021

J of Applied Toxicology

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“…All of green synthesized AgNP solutions showed significant decrease in DPPH production, thus demonstrating the residual presence of phenolic compounds after the reduction of Ag + ions. The profound antioxidant properties of synthesized AgNPs may significantly attenuate their cytotoxicity, thus widening the therapeutic potential of nanoparticles (Barbasz et al, 2021). (Franci et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Biosynthesis of silver nanoparticles with antioxidant and bactericidal activities using Capsicum annuum fruit extract

Kalynovskyi,

Smirnov,

Zelena

et al. 2023

Regul. Mech. Biosyst.

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Metallic nanostructures, especially silver nanoparticles (AgNPs) have already found multiple applications in modern industry, science and medicine. Still, the production of nano-sized compounds often leads to the formation of toxic byproducts and possesses substantial environmental hazard. One of the promising solutions for the ecofriendly creation of nanomaterials predicts the use of a “green chemistry” approach using organisms, their parts or natural compounds to act as safe and effective producers of nanomaterials. Plant-mediated biosynthesis of silver nanoparticles includes the reduction of Ag+ to Ag0 by natural compounds, usually secondary metabolites which can be found in roots, leaves, cortex, fruits, flowers and seeds of various species. Aqueous extract of Capsicum annuum var. cv. (cultivar) Teja (S-17) fruits was used as a bioreducer for the reduction of AgNO3 solution to AgNPs. The formation of the AgNPs was confirmed by the presence of the Tyndall effect of light scattering combined with colour change of the solutions. The properties of the nanoparticles were assessed with UV-visible spectroscopy and scanning electron microscopy. In the present study, we report the experimental optimization of operating parameters needed for silver biotransformation by C. annuum. Biosynthesized nanoparticles were 13–22 nm in size and spherical in shape. Colloidal solutions of AgNPs were also confirmed to show antioxidant activity in vitro as analyzed by the reduction of DPPH radicals. Characterization and application of AgNPs as bactericidal agents on two Gram-positive (Micrococcus luteus, Staphylococcus aureus) and two Gram-negative (Escherichia coli, Pseudomonas aeruginosa) prokaryotic microorganisms demonstrated the prevalent influence on Gram-positive strains.

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“…An additional factor may be at play here, namely that antioxidants are able to reduce their cytotoxicity [ 51 , 52 , 53 ]. Although these studies showed that the introduction of additional antioxidants, such as ascorbic acid (vitamin C) and quercetin, can reduce the toxicity of Ag NPs, there is no report on the relationship between the NP cytotoxicity and antioxidant performance.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity and Antibacterial Efficacy of AgCu and AgFe NanoAlloys: A Comparative Study

Zhou¹,

Kostantin

Yang

et al. 2022

Antibiotics

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Although Ag nanoparticles (NPs) have been widely applied in daily life and in biomedical and industrial fields, there is a demand for Ag-based bimetallic nanoalloys (NAs), such as AgCu and AgFe, due to their enhanced antibacterial efficacy and reduced Ag consumption. In this work, we present a comparison study on the antibacterial efficacy and cytotoxicity rates of Ag NPs and AgCu and AgFe NAs to L929 mouse fibroblast cells using the CCK-8 technique based on the relative cell viability. The concept of the minimum death concentration (MDC) is introduced to estimate the cytotoxicity to the cells. It is found that the minimum inhibitory concentrations (MICs) of the NPs against E. coli and S. aureus decrease with the addition of both Cu and Fe. There is a strong correlation between the MDC and MIC, implying that the mechanisms of both antibacterial efficacy and cytotoxicity are similar. The enhanced antibacterial efficacy to bacteria and cytotoxicity toward the cell are attributed to Ag+ release. The following order is found for both the MIC and MDC: AgFe < AgCu < Ag NPs. However, there is no cytotoxicity to the L929 cells for AgFe and AgCu NAs at their MIC Ag concentrations against S. aureus.

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Antioxidant‐modulated cytotoxicity of silver nanoparticles

Cited by 13 publications

References 56 publications

Design cytotoxicity: The effect of silver nanoparticles stabilized by selected antioxidants on melanoma cells

Design cytotoxicity: The effect of silver nanoparticles stabilized by selected antioxidants on melanoma cells

Biosynthesis of silver nanoparticles with antioxidant and bactericidal activities using Capsicum annuum fruit extract

Cytotoxicity and Antibacterial Efficacy of AgCu and AgFe NanoAlloys: A Comparative Study

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