2020
DOI: 10.1007/s11033-020-05276-y
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Antioxidant molecular mechanism of adenosyl homocysteinase from cyanobacteria and its wound healing process in fibroblast cells

Abstract: An antioxidant molecule namely, adenosyl homocysteinase (AHc) was identified from the earlier constructed transcriptome database of Spirulina, where it was cultured in a sulphur deprived condition. From the AHc protein, a small peptide NL13 was identified using bioinformatics tools and was predicted to have antioxidant property. Further, the peptide was synthesised and its antioxidant mechanism was addressed at molecular level. NL13 was subjected to various antioxidant assays including DPPH assay, HARS assay, … Show more

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Cited by 35 publications
(21 citation statements)
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“…In hydroxyl radical scavenging assay, the activity of RM12 peptide is strong and it reacts with biomolecules and helps to remove the hydroxyl radical in diseases (Xie et al 2008 ). Proline was reported to have hydroxyl radical scavenging ability (Sarkar et al 2020a , b ). As RM12 peptide contains proline amino acid (16%), RM12 peptide may exhibit a potent radical scavenging activity.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…In hydroxyl radical scavenging assay, the activity of RM12 peptide is strong and it reacts with biomolecules and helps to remove the hydroxyl radical in diseases (Xie et al 2008 ). Proline was reported to have hydroxyl radical scavenging ability (Sarkar et al 2020a , b ). As RM12 peptide contains proline amino acid (16%), RM12 peptide may exhibit a potent radical scavenging activity.…”
Section: Discussionmentioning
confidence: 99%
“…The samples were treated with RM12 peptide for 1h at room temperature. After incubation, the samples were centrifuged and the supernatant were used to measure the reading at 540nm (Le et al 2015 ; Sarkar et al 2020b , a ). Triton X and PBS were used as positive and negative controls respectively.…”
Section: Methodsmentioning
confidence: 99%
“…PMC 882.14 extract stimulated slightly keratinocyte migration on the HaCaT cell line. Several cyanobacterial extracts have been studied in recent years for their potential wound-healing effects [ 67 , 68 ], but the mechanism of action of most of these compounds remains unclear. The aqueous extract of Aliinostoc sp.…”
Section: Discussionmentioning
confidence: 99%
“…Nevertheless, its concentration may rise substantially as a reflection of increased activation of cellular metabolism ( Eltzschig, 2013 ). Adenosine is formed in the cells as an outcome of the enormous consumption of ATP, the increased number of methylation events, as well as generation of reductive potential ( Ham and Evans, 2012 ; Tehlivets et al, 2013 ; Sarkar et al, 2020 ). Accumulation of intracellular adenosine serves as a negative feedback signal on cellular metabolism via AMPK activation leading to quiescence ( Aymerich et al, 2006 ).…”
Section: Adoption Of Aerobic Glycolysis Is Connected With the Releasementioning
confidence: 99%