Antioxidant potency of n-butanol fraction of Ficus glumosa leaves against oxidative stress induced by carbon tetrachloride in the kidneys of rats

Abstract

Abstract. Abu MS, Yakubu OE, Onuche JI, Tatah SV. 2022. Antioxidant potency of n-butanol fraction on Ficus glumosa leaves against oxidative stress induced by carbon tetrachloride in the kidneys of rats. Nusantara Bioscience 14: 40-46. The kidneys play several essential roles in the body, including regulating the water and ions reabsorb from glomerular filtrate in kidney tubules. It is controlled by several hormones such as antidiuretic hormone (ADH), aldosterone, and angiotensin II. This report presented the repairing effect of n- butanol fraction of Ficus glumosa Delile on nephrotoxicity induced by CCl4 in rats. Rats were divided into 7 groups of 5 animals each. Group 1 and 2 were used as normal and vehicle controls, respectively. Group 3 was induced but treated with neither extract nor standard drug. However, Groups 3, 4, and 5 were induced by CCl4 and administered with varying doses of the n-butanol fraction. Group 6 was induced by CCl4 and treated with a standard antioxidant drug. The results showed that treatments with an n-butanol fraction of F. glumosa leaves and silymarin significantly (p<0.05) restore the activities of SOD, GPx, and CAT comparable to normal values, i.e., 2.10±0.07 U/L, 43.8±2.49 U/L, and 34.2±2.59 U/L, respectively. In addition, the treatments reduce the MDA level in the kidney of rats treated with n-butanol fraction comparable to 0.34±0.05 mmol/L in the normal rats. Similarly, there was a significant (p<0.05) reduction of urea from 5.24 mg/dL to 3.5 mg/dL (standard value) in the treated groups. Creatinine significantly (p<0.05) reduced from 71.2 mg/dL in the treated groups to 43.3 mg/dL in the normal group. Electrolytes, Na+, K+, and Cl- levels in the CCl4-induced rats after administration of n-butanol fraction of F. glumosa leaves significantly (p<0.05) decreased to 137.8±2.59 mmol/L, 3.98±0.54 mmol/L, and 92.8±1.92 mmol/L respectively. The significant reversal of the rats' biochemical parameters can be ascribed to the ameliorative potency of the n-butanol fraction of methanol extract of F. glumosa leaves on the glomerular and tubular cells, which may have improved renal function in the injured kidney.

Antidiabetic and Hepatoprotective Effects of Aqueous and Ethanolic Leaf Extracts of Mitracarpus Hirtus on Alloxan-Induced Diabetic Rats

“…Similarly, ALT also significantly decreased when group 11 (53.46 ± 4.6 U/L) was compared with group 2 (101.42 ± 16.01 U/L). This report agreed with Ugwu et al (2013); Yakubu et al, (2020) and Abu et al, (2022). The decrease in AST and ALT in the treatments groups may be attributed to the presence of flavonoids which possess a wide range of biological benefits, including organ protection, hypoglycemic, lipid-lowering, anti-oxidative, and anti-inflammatory properties (López-Lázaro, 2002).…”

Anti-nephrotoxic activity of aqueous extract of polyherbal mixture against renal toxicity induced by paracetamol in Wistar albino rats

“…This result can be deduced that administering the evaluated polyherbal extract can ameliorate the elevated level of serum electrolytes orchestrated by paracetamol toxicity, probably due to some bioactive components such as polyphenol, glycoside, and flavonoid in the different plants. The normalization of electrolytes in this study could liken to the findings of Abu et al (2022), that used an n-butanol fraction of F. glumosa to reverse the distorted levels of sodium, chloride, and potassium ions in albino rats.…”

Effect of Aqueous n-Butanol Treatments on Shelf-Life Extension of Longkong Fruit during Ambient Storage

“…Tanko et al [52] observed that the application of n-butanol had increased the antioxidant enzymes in the Indiogfera pulchara plants. In another study, Abu et al [53] also found that the application of n-butanol had significantly increased the antioxidant potency of Ficus glumosa leaves. Chen et al [54] reported that the application of n-butanol in Ligustrum robustum induced the production of various antioxidant genes to deal with MDA and hydrogen peroxide.…”