ANTIOXIDANT POTENTIAL OF EEL (ANGUILLA JAPONICA AND CONGER MYRIASTER) FLESH AND SKIN

Abstract

The flesh and skin of Anguilla japonica and Conger myriaster were investigated for their antioxidant activity. Ethyl acetate and diethyl ether extracts (4 mg/mL) after extracting with methanol were tested for 2,2‐diphenyl‐1‐picrylhidrazyl (DPPH) free radical scavenging (FRS) activity for flesh and skin of each eel species. The values were compared with α‐tocopherol and butylated hydroxytoluene (BHT). Extracts showing positive results, when tested for DPPH FRS, were examined for dose effect, hydrogen peroxide (H2O2), hydroxyl radical and superoxide scavenging assays and heat stability. All extracts of A. japonica showed a dose‐dependent DPPH FRS and significant hydroxyl radical scavenging activities (>65%). The diethyl ether extract of the flesh of A. japonica showed the highest superoxide scavenging activity. Diethyl ether extracts of A. japonica were heat stable, and ethyl acetate extracts were stable up to 75C. Thus, it can be concluded that A. japonica is rich with heat stable and nonpolar antioxidants.

Effects of laurel and myrtle extracts on the sensory, chemical and microbiological properties of vacuum‐packed and refrigerated European eel (Anguilla anguilla) fillets

“…Ekanayake et al . () reported protein (19.10%), lipid (31.50%) and moisture (56.20%) contents of fresh eels. Vishwanath et al .…”

Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor

“…The assay was done according to the modified method of Ekanayake et al (2005). In the first series the modification was the mixing of each sample extract (2 mL) with 1 mL freshly prepared DMSO solution of 0.2 mM DPPH.…”

Antioxidative activities and chemical characterization of polysaccharides extracted from the basidiomycete Schizophyllum commune

“…The assay was done according to the modified method of Ekanayake et al (2005). In the first series the modification was the mixing of each sample extract (2 mL) with 1 mL freshly prepared DMSO solution of 0.2 mmol/L DPPH.…”