Antioxidant Potential of Ethanol and Ethyl Acetat Extract of Ganoderma sp. Mycelium

Ratnaningtyas, Nuniek Ina; Purnomowati, Purnomowati; Purwati, Endang Sri; Septiana, Aisyah Tri; Ekowati, Nuraeni; Supriyadi, Adi

doi:10.15294/biosaintifika.v10i1.11512

Cited by 4 publications

(4 citation statements)

References 8 publications

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“…Ganoderma contains bioactive compound which can synthesize glutathione (GSH). GSH is naturally found in almost all mammal tissues, particularly concentrated in their heart, and potentially serves to be antioxidant (Ratnaningtyas, 2018). Alloxan reduction into dialuric acid and ROS production lead to GSH decrease since alloxan binds -SH group from glutathione which cause decreasing glucokinase enzyme activity, thus inhibiting the glycolysis process.…”

Section: Resultsmentioning

confidence: 99%

Effects of Ganoderma lucidum Extract on Diabetic Rats

et al. 2018

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Diabetes mellitus (DM) is a metabolic syndrome which occurs when insulin is insufficiently produced or insulin cannot well serve its function. Diabetes is marked with increase in blood glucose level followed by increase in glycosylated hemoglobin level and decrease in insulin level. This research aims to examining the effect of Ganoderma lucidum extract on the blood glucose, insulin and glycosylated hemoglobin (HbA1c) level of diabetic white rat and determining the most effective dose of extract to be a diabetic agent. This research was experimentally conducted by employing Completely Randomized Design (CRD) with 6 treatments and 4 repetitions. The treatment groups consisted of healthy rats group (K1), rats with diabetes as negative control (K2), diabetic rats with the administration of metformin 45 mg/kg BW as the comparison (K3) and diabetic rats with the administration of G. lucidum extract with dose of 250, 500 and 1000 mg /kg BW (K4, K5 and K6 respectively). Blood glucose level examination was conducted after the alloxan induction with single dose of 125 mg/kg BW by intraperitoneal injection. The results show that mushroom G. lucidum extract administration with dose of 1000 mg/kg BW (K6) is the best dose to be an anti-diabetic agent. The benefit of the research is developing anti-diabetic agent from herbal resources.

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Section: Resultsmentioning

confidence: 99%

Effects of Ganoderma lucidum Extract on Diabetic Rats

et al. 2018

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“…Karakteristik molekul yang demikian, memungkinkan pelarut polar seperti etanol 70% mampu mengekstrak senyawa polifenol, secara optimal. Etil asetat merupakan pelarut semi polar, sehingga dapat menarik senyawa-senyawa golongan fenolik seperti flavonoid, tanin dan golongan terpenoid, yang termasuk kelompok semipolar [30]. Kelompok senyawa fenolik yang dapat ditarik oleh pelarut semipolar, umumnya merupakan golongan tanin yang terkondensasi, sedangkan golongan terpenoid, umumnya akan berada dalam range senyawa nonpolar hingga semipolar.…”

Section: Serbukunclassified

Antibacterial Activity of Grape Seeds Extracts (Vitis vinifera L.) Against Streptococcus mutans ATCC 31987

et al. 2021

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ABSTRAK Streptococcus mutans merupakan salah satu bakteri penyebab karies gigi. Penggunaan antibiotic merupakan terapi infeksi yang umum dilakukan untuk menghambat pertumbuhan bakteri. Biji anggur diketahui mengandung senyawa polifenol yang berpotensi sebagai antibakteri. Tujuan penelitian ini untuk mengetahui aktivitas ektrak biji anggur terhadap Streptococcus mutans. Bahan uji yang digunakan adalah buah anggur yang diambil bijinya. Buah anggur diperoleh dari Pasar Induk, Kramat Jati, Jakarta Timur. Ekstrak biji anggur diperoleh dengan metode maserasi menggunakan pelarut etil asetat dan etanol 70%. Uji aktivitas antibakteri dilakukan menggunakan metode difusi cakram pada media Mueller Hinton Agar dengan konsentrasi 5%, 10%, 20%, dan 40%. Hasil penelitian menunjukkan bahwa ekstrak etanol 70% biji anggur memiliki aktivitas antibakteri pada konsentrasi 5%, 10%, 20%, dan 40% dengan nilai hambatan secara berurutan sebesar 8,46 mm; 8,91 mm; 9,89 mm; dan 11,04 mm. Hasil pada ekstrak etil asetat juga menunjukkan ada aktivitas penghambatan pada konsentrasi 5%, 10%, 20%, dan 40% dengan nilai hambatan secara berurutan sebesar 7,72 mm; 8,50 mm; 9,64 mm; dan 10,51 mm. Ekstrak etanol 70% biji anggur memiliki daya hambat yang lebih besar dibandingkan dengan ekstrak etil asetat. Hasil ini dapat menjadi acuan untuk pengembangan potensi biji anggur sebagai bahan obat atau kosmedik (kosmetik-medik) penanganan karies gigi. ABSTRACT Streptococcus mutans is one of the bacteria that cause dental caries. The antibiotics usually used for the therapy of this infection to inhibit bacterial growth. Polyphenol, which recommended as an antibacterial agent, is common secondary metabolites in grape seeds. The purpose of this study is to know the activity of grape seed extract against Streptococcus mutans. The grape seeds were obtained from fresh fruits which bought from Pasar Induk, Kramat Jati, East Jakarta. The grape seed extract was obtained by the maceration method using ethyl acetate and 70% ethanol. The antibacterial activity test was carried out using the disk diffusion method on Mueller Hinton Agar media with a concentration of 5%, 10%, 20%, and 40%. The results showed that 70% ethanol extract of grape seeds had antibacterial activity at concentrations of 5%, 10%, 20%, and 40% with a value of 8.46 mm; 8.91 mm; 9.89 mm; and 11.04 mm respectively. The results of ethyl acetate extract also showed inhibitory activity at concentrations of 5%, 10%, 20%, and 40% with values of 7.72 mm; 8.50 mm; 9.64 mm; and 10.51 mm respectively. The inhibition of 70% ethanol extract of grape seed is greater than ethyl acetate extract. The results of the study can be used as reference for the potential development of grape seed as a medicinal or cosmedic cosmetic-medical) ingredient for treating dental caries.

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“…Sources of active compounds can use the Ganoderma lucidum mushroom which is a medicinal mushroom (Ratnaningtyas, 2007). The use of a dose of 500 mg.kg BB -1 extract of G. lucidum was able to provide a protective effect for the kidneys (nephroprotective) against cadmium in terms of kidney function (Yang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Detoxification of Cadmium on Albino Rats (Rattus norvegicus) with Natural Chelator of Fruiting Body Extract of Ganoderma lucidum

Taufiq

Hernayanti²,

Ratnaningtyas³

2023

molekul

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Cadmium is a heavy metal pollutant sourced from various industries and toxic to the kidneys. Cadmium exposure can be used natural chelator of ethanol extract of the fruiting body of Ganoderma lucidum. The aim of the study was to determine the effect and effective dose of the ethanolic extract of the fruiting body of G. lucidum on reducing the toxicity effect of cadmium in male albino rats (Rattus norvegicus) Wistar strain. The research design was experimental research, which consisted of 5 treatment levels. The research parameters were blood cadmium, β2-microglobulin, malondialdehyde, and superoxide dismutase levels. The ethanolic extract of fruiting body of G. lucidum was tested by gas chromatography-mass spectrometry (GCMS) to determine linalool as active compounds. The results of each parameter had a significant effect, which decreased blood cadmium, β2M and MDA levels, and increased SOD level that linear according to the addition of the dose of ethanol extract of the fruiting body of G. lucidum. Blood cadmium levels with β2M and MDA levels had a positive correlation, while blood cadmium levels with SOD levels had a negative correlation. The dominant compound detected was linalool which has potential as a radical scavenger. The dose of 750 mg.kgBW-1 is the effective dose of the ethanolic extract of fruiting body of G. lucidum based on a decrease in blood cadmium levels (54.10%), β2M (63.94%) and MDA (20.31%), as well as an increase in SOD levels (14.20%) compared to sick control.

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Antioxidant Potential of Ethanol and Ethyl Acetat Extract of Ganoderma sp. Mycelium

Cited by 4 publications

References 8 publications

Effects of Ganoderma lucidum Extract on Diabetic Rats

Effects of Ganoderma lucidum Extract on Diabetic Rats

Antibacterial Activity of Grape Seeds Extracts (Vitis vinifera L.) Against Streptococcus mutans ATCC 31987

Detoxification of Cadmium on Albino Rats (Rattus norvegicus) with Natural Chelator of Fruiting Body Extract of Ganoderma lucidum

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