Antioxidant Potential of Pine Needles: A Systematic Study on the Essential Oils and Extracts of 46 Species of the Genus Pinus

Koutsaviti, Aikaterini; Toutoungy, Samer; Saliba, Rouba; Loupassaki, Sofia; Τζακου, Ολγα; Roussis, Vassilios; Iοannou, Efstathia

doi:10.3390/foods10010142

Cited by 23 publications

(14 citation statements)

References 48 publications

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“…Pinus is a well-known source of antioxidants, mainly phenolic compounds. 24 Lately, the antimicrobial, antidiabetic, anti-inflammatory, and cytotoxic properties of P. halepensis have been reported. [25][26][27][28] To the best of our knowledge, P. halepensis bark extract has not been studied before with prostate cancer or prostate cancer PDT.…”

Section: Discussionmentioning

confidence: 99%

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Assessment of natural antioxidants' effect on PDT cytotoxicity through fluorescence microscopy image analysis

2021

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Background and Objectives Photodynamic therapy (PDT) is a cancer treatment modality mediated by reactive oxygen species (ROS). However, the intracellular antioxidant defense system antagonizes PDT‐generated ROS, impeding PDT efficacy. This study aimed to evaluate the enhancement of PDT cytotoxicity by its combination with natural antioxidants in pro‐oxidant concentrations. Methods A rich natural antioxidant mixture originating from Pinus halepensis bark extract was studied for its potential to enhance the efficacy of m‐tetrahydroxyphenylchlorin (m‐THPC)–PDT on LNCaP prostate cancer cells, in vitro. Various P. halepensis concentrations, at two different incubation times, were used in combination with m‐THPC–PDT. Assessment of cellular viability and intracellular ROS levels evaluated the treatments' outcome. A novel method was developed for the assessment of the intracellular ROS levels, based on image analysis and data extraction from fluorescence microscopy images. Results P. halepensis bark extract increased the intracellular ROS levels in a concentration‐dependent but not in an incubation‐dependent manner. The higher concentrations used (≥50 μg/ml) reduced cellular viability even by 50%. One hour pretreatment with 30 μg/ml P. halepensis before m‐THPC–PDT exceeded the levels of cellular death by approximately 15%. Conclusions The results provided evidence of the cytotoxic effect of P. halepensis bark extract on LNCaP cells, showing the potential of P. halepensis to be used as an anticancer agent in prostate cancer treatment. The results also provided evidence of enhancement of m‐THPC–PDT by P. halepensis bark extract showed the potential to be used as a supplementary agent to improve prostate cancer PDT treatment.

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Section: Discussionmentioning

confidence: 99%

“…Pinus is a well‐known source of antioxidants, mainly phenolic compounds 24 . Lately, the antimicrobial, antidiabetic, anti‐inflammatory, and cytotoxic properties of P. halepensis have been reported 25–28 .…”

Section: Discussionmentioning

confidence: 99%

Assessment of natural antioxidants' effect on PDT cytotoxicity through fluorescence microscopy image analysis

2021

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“…Pine trees are widely used to produce various wood and cellulose products, but often overlooked are the needles of these trees. In scientific literature, pine needles have been studied to monitor atmospheric pollution [ 14 , 15 , 16 ], while needle extracts are used in various medications and supplements [ 17 , 18 ]. Natural waxes are a byproduct of this extraction, often treated as waste.…”

Section: Introductionmentioning

confidence: 99%

Sustainable Wax Coatings Made from Pine Needle Extraction Waste for Nanopaper Hydrophobization

et al. 2022

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We combine renewable and waste materials to produce hydrophobic membranes in the present work. Cellulose nanopaper prepared from paper waste was used as a structural component for the membrane. The pine wax was reclaimed from pine needle extraction waste and can be regarded as a byproduct. The dip-coating and spray-coating methods were comprehensively compared. In addition, the solubility of wax in different solvents is reported, and the concentration impact on coating quality is presented as the change in the contact angle value. The sensile drop method was used for wetting measurements. Spray-coating yielded the highest contact angle with an average of 114°, while dip-coating reached an average value of 107°. Scanning electron microscopy (SEM) was used for an in-depth comparison of surface morphology. It was observed that coating methods yield significantly different microstructures on the surface of cellulose fibers. The wax is characterized by nuclear magnetic resonance (NMR) spectroscopy and differential scanning calorimetry (DSC). Pine wax has a melting temperature of around 80 °C and excellent thermal stability in oxygen, with a degradation peak above 290 °C. Fourier transform infrared spectroscopy (FTIR) was used to identify characteristic groups of components and show the changes on coated nanopaper. Overall, the results of this work yield important insight into wax-coated cellulose nanopapers and a comparison of spray- and dip-coating methods. The prepared materials have a potential application as membranes and packaging materials.

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“…reported that pine needles has many physiological, pharmacologial activities and it is a good source of antioxidants and phenolic compounds including phenolic acids, flavonoids, procyanides [1], [2]. Pine trees (Pinus densiflora) have shown a wide range of biologically functional activities and many methods have been applied for phenolic compounds extraction from pine trees.…”

Section: Introductionmentioning

confidence: 99%

Quality control of pine needles extracts (PNE) by using electroanalytical methods

Sefer¹,

Keran²,

Ahmetović³

et al. 2022

IJEAB

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pH represents the concentration of free H+ in pine needles extracts (PNE) and is therefore an important initial parameter in quality control. Electrical conductivity and pH of samples of fresh and stored for 20 days of PNE with black cumin oil and olive oil had values of 0.00 due to the encapsulation of water molecules, pH and electrical conduction was not possible. The pH of the other samples was in a weakly acidic environment because the pH of natural pine needles is 3.8. Electrical conductivity values in all samples except pine needle extract and honey increased during storage. By monitoring the parameters of pH and electrical conductivity in the quality control of PNE, it gives us a significant insight into the physical state of the phases and the way of storage.

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Antioxidant Potential of Pine Needles: A Systematic Study on the Essential Oils and Extracts of 46 Species of the Genus Pinus

Cited by 23 publications

References 48 publications

Assessment of natural antioxidants' effect on PDT cytotoxicity through fluorescence microscopy image analysis

Assessment of natural antioxidants' effect on PDT cytotoxicity through fluorescence microscopy image analysis

Sustainable Wax Coatings Made from Pine Needle Extraction Waste for Nanopaper Hydrophobization

Quality control of pine needles extracts (PNE) by using electroanalytical methods

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