Antioxidant potential of silk protein sericin against hydrogen peroxide-induced oxidative stress in skin fibroblasts

Dash, Rupesh; Acharya, Chitrangada; Bindu, P.; Kundu, Subhas C.

doi:10.5483/bmbrep.2008.41.3.236

Cited by 175 publications

(123 citation statements)

References 35 publications

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“…It has been shown that sericin has a strong antioxidant activity that suppresses lipid peroxidation and inhibits tyrosinase activity (Kato et al, 1998). Dash et al (2008) demonstrated that sericin prevented oxidative stress in a skin fibroblast cell line when exposed to hydrogen peroxide and inhibited the production of intracellular hydrogen peroxide in keratinocytes. Our group has recently reported that sericin increased the preimplantation development and quality of bovine embryos produced in vitro and reduced the oxidative stress exerted by hydrogen peroxide on embryos in culture (Isobe et al, 2012).…”

mentioning

confidence: 99%

Maturation and fertilisation of sheep oocytes cultured in serum-free medium containing silk protein sericin

Yasmin

Otoi

Setiadi

et al. 2015

Acta Veterinaria Hungarica

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Sericin is a water-soluble component of silk and has been used as a biomaterial due to its antibacterial and ultraviolet radiation-resistant properties. This study was designed to evaluate the effect of sericin supplementation in a maturation medium on the meiotic competence and fertilisability of sheep oocytes. Cumulus-oocyte complexes (COCs) were cultured in TCM199 supplemented with sericin at various concentrations of 0 (control), 0.1, 0.25 and 0.5%, either with or without bovine serum albumin (BSA). When the COCs were matured without BSA, the supplementation of 0.1% sericin significantly increased the rates of maturation to metaphase II and the total fertilisation of oocytes compared with the other concentrations of sericin. When the COCs were matured with BSA, the beneficial effects of 0.1% sericin supplementation on the maturation and fertilisation of oocytes were not observed. Our findings indicate that supplementation with 0.1% sericin during maturation culture may improve the nuclear maturation and fertilisability of sheep oocytes. Moreover, it may be possible to replace BSA with sericin in chemically defined media without the risk of disease transmission.

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mentioning

confidence: 99%

Maturation and fertilisation of sheep oocytes cultured in serum-free medium containing silk protein sericin

Yasmin

Otoi

Setiadi

et al. 2015

Acta Veterinaria Hungarica

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“…이와 같은 부작용을 완화시키 기 위한 방편으로 친환경 소재 개발 연구가 진행되고 있 으며 특히, 여러 천연 물질들의 미백, 항산화, 항균, 보습 효과 등이 과학적으로 입증되면서 이들에 대한 연구가 확 대되고 있다 (Mukherjee et al 2011, Choi et al 2010 (Yeo et al 2000, Dash et al 2008, Lee et al 2001 (Zhang et al 2011, Kim et al 2003, Choi et al 2002. (Fig.…”

Section: 서 론unclassified

Effects of matured silkworm hemolymph on suppressing melanin synthesis

Mun¹,

Lee²,

Lee³

et al. 2013

Korean Journal of Sericultural Science

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In this study, matured silkworm hemolymph was studied for inhibitory effect on melanin formation, tyrosinase activities. A matured silkworm hemolymph mass purification method was developed and some of suppressing melanin formation effects were investigated, too. As a result, the advanced purification method was higher in yield rate than existing one by 53%. A free radical scavenging activity of hemolymph was higher than sericin on the market(IC 50 , 202.9 ± 33.1) and lower than vitamin C(0.57 ± 0.02) by 6.4 ± 0.3 ㎍/㎖. An inhibitory effect on melanin synthesis was higher in hemolymph compared to Morus bark and arbutin by 9.15% and 11.56%, respectively. An inhibitory effect on tyrosinase activity was higher in hemolymph than Morus bark and lower than arbutin. Effect of hemolymph 45 ㎍/㎖ on suppressing of tyrosinase was almost 90%. Thus, these results suggest that hemolymph of matured silkworm may have beneficial properties as a material for cosmetics.

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“…Cell viability was then determined by the same microplate reader at 550 nm and the concentration of H 2 O 2 that resulted in cell damage was obtained. This concentration was then applied to NHF cells (8 × 10 3 cells/well) for 24 h. After treatment, the cells were incubated with the extracts (5 and 10 μg/ mL) for another 24 h. Determination of cell viability was conducted as described in cytotoxicity against a NHF cell culture section (Dash et al, 2008).…”

Section: Cellular Oxidative Damage Treatmentmentioning

confidence: 99%

“…Cytotoxicity in NHF cells was determined using a method described by Dash et al (2008). NHF cell lines obtained from the National Nanotechnology Center were incubated at 37°C in a chamber containing 5% CO 2 .…”

Section: Cytotoxicity Against a Normal Human Fibroblast (Nhf) Cell Cumentioning

confidence: 99%

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Biological activity assessment and phenolic compounds characterization from the fruit pericarp ofLitchi chinensisfor cosmetic applications

Kanlayavattanakul

Ospondpant

Ruktanonchai

et al. 2012

Pharmaceutical Biology

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Antioxidant potential of silk protein sericin against hydrogen peroxide-induced oxidative stress in skin fibroblasts

Cited by 175 publications

References 35 publications

Maturation and fertilisation of sheep oocytes cultured in serum-free medium containing silk protein sericin

Maturation and fertilisation of sheep oocytes cultured in serum-free medium containing silk protein sericin

Effects of matured silkworm hemolymph on suppressing melanin synthesis

Biological activity assessment and phenolic compounds characterization from the fruit pericarp ofLitchi chinensisfor cosmetic applications

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