Antioxidant properties and structured biodiversity in a diverse set of wild cranberry clones

Abstract

Wild germplasm with elevated antioxidants are a useful resource for using directly and in a breeding program. In a study with 136 wild clones and two cranberry cultivars, phenolic, flavonoid and anthocyanin contents varied 2.79, 2.70 and 17.46 times, respectively. The antioxidant activity ranged from 1.17 ± 0.01 to 2.53 ± 0.05 mg/g and varied 2.16 times. Seventy-five of wild clones and the cultivar Franklin were grouped into five distinct classes by molecular structure analysis using inter simple sequence repeat (ISSR), expressed sequence tag-simple sequence repeat (EST-SSR) and EST-polymerase chain reaction (PCR) markers. Grouping with DNA markers did not coincide with that of based on antioxidant properties. Present study indicates that genetic diversity analysis combined with antioxidant properties of wild germplasm play a significant role for conservation and in selecting diverse genotypes for future berry crop improvement.

Genetic Diversity of Blueberry Genotypes Estimated by Antioxidant Properties and Molecular Markers

“…In their study, Debnath and Sion [ 80 ] reported no correlation between genetic diversity based on ISSR markers and chemical diversity based on antioxidant activity and anthocyanin content in lingonberry. Similar observations were also reported in strawberry [ 81 ] and cranberry using ISSR, EST–SSR, and EST–PCR markers [ 82 ]. We also studied the association of EST–SSR, G–SSR and EST–PCR markers with 70 genotypes of blueberry and found that only one marker was associated with TPC, as revealed by DPPH assay, and five markers were associated with both TPC and TFC.…”

Cranberry: Chemical Composition, Antioxidant Activity and Impact on Human Health: Overview

“…The content of total anthocyanins ranged from 695 to 1716 mg/100 g dm for the six cultivars of Vaccinium macrocarpon L. grown in Poland [ 12 ]. Of one hundred and thirty-six wild cranberry fruits, Vacciniun macrocarpon Aiton, and two cultivars, ‘Franklin’ and ‘Bergman’, Debnath and An [ 22 ] observed the total anthocyanins content for all cranberry genotypes to differ significantly ( p < 0.05). Variation ranged from the least amount of total anthocyanins in the wild clone cranberry NL77 of 0.13 mg/g fw to the highest amount detected in the genotype ‘Franklin’ of 2.27 mg/g fw [ 22 ].…”

“…Of one hundred and thirty-six wild cranberry fruits, Vacciniun macrocarpon Aiton, and two cultivars, ‘Franklin’ and ‘Bergman’, Debnath and An [ 22 ] observed the total anthocyanins content for all cranberry genotypes to differ significantly ( p < 0.05). Variation ranged from the least amount of total anthocyanins in the wild clone cranberry NL77 of 0.13 mg/g fw to the highest amount detected in the genotype ‘Franklin’ of 2.27 mg/g fw [ 22 ]. In another study by Česonienė et al [ 15 ], six anthocyanins were identified in the two species of cranberries, Vaccinium macrocarpon and Vaccinium oxycoccos .…”

“…Abeywickrama et al [ 19 ] detected cyanidin-3-O-arabinoside and cyanidin-3-O-galactoside in the cranberry Canadian wild clone NL2 and ‘Pilgrim’. This shows that the anthocyanins concentration in cranberry fruits is dependent on cultivar and ripening stage [ 12 , 13 , 22 ].…”

“…The flavonol glycosides identified in Vaccinium vitisidaea cranberry by Diaconeasa et al [ 23 ] were myricetin-galactoside, quercetin-rutinoside (rutin), myricetin-arabinoside, myricetin-glucoside, quercetin-galactoside, quercetin-acetyl-glucoside, quercetin-glucoside and quercetin-rhamnoside. Debnath and An [ 22 ] showed significant differences ( p <0.05) in the total flavonoid content among the 136 wild clones and two cultivars ranging from 2.78 to 7.51 for the wild clones, NL51 and NL92, respectively. The cultivar ‘Franklin’ (5.81 mg/g fw) had more total flavonoids than ‘Bergman’ (4.06 mg/g fw) [ 22 ].…”