Antioxidant properties of components extracted from puccoon (Lithospermum erythrorhizon Sieb. et Zucc.)

Weng, Xinchu; Xiang, Gao; Ai-li, Jiang; Liu, Y.P; Wu, Lidong; Dong, Xue; Duan, Shukai

doi:10.1016/s0308-8146(99)00236-8

Cited by 31 publications

(24 citation statements)

References 3 publications

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“…1 H and 13 C spectral assignments of isolated compounds, recorded in CDCl 3 , at 300 MHz (Bruker Avance-300 spectrophotometer, Switzerland), along with their HREIMS data (Waters, USA) are given below and were found to correlate well with previous reports [46,47]. C NMR data for peak at R t = 6.9 (Fig.…”

Section: Spectroscopic Data Of Isolated Compoundssupporting

confidence: 78%

gIsolation and purification of acetylshikonin and β‐acetoxyisovalerylshikonin from cell suspension cultures of Arnebia euchroma (Royle) Johnston using rapid preparative HPLC*

Sharma

Malik

et al. 2008

J of Separation Science

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Shikonin and its derivatives are important red colored naphthoquinone pigments found in a large number of Arnebia species, including A. euchroma, that are responsible for the various pharmacological activities exhibited by the plant. The precise separation of each naphthoquinone is essential for total quality evaluation and bioactivity analysis of herbal formulations of A. euchroma. Furthermore, the overexploitation of this useful plant has resulted in species becoming endangered. With this in mind, a simple and rapid preparative scale HPLC method with single compound recovery for the isolation and purification of two shikonin derivatives (i. e. acetylshikonin, beta-acetoxyisovalerylshikonin) from cell suspension cultures of A. euchroma is presented. The compounds were separated on a C(18) column within 10 min using acetonitrile/methanol (95:5) as mobile phase in isocratic mode. The isolated compounds were found to be more than 98% pure. The LOD for acetylshikonin and beta-acetoxyisovalerylshikonin was estimated at 0.063 and 0.146 mug/mL, respectively, while the LOQ was found to be 0.209 and 0.487 mug/mL, respectively. The recoveries accomplished for both the shikonin derivatives were in the range of 94.7-96.8%. The repeatability, expressed as %RSD, of acetylshikonin and beta-acetoxyisovalerylshikonin was found to be 1.74 and 1.27, respectively.

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Section: Spectroscopic Data Of Isolated Compoundssupporting

confidence: 78%

gIsolation and purification of acetylshikonin and β‐acetoxyisovalerylshikonin from cell suspension cultures of Arnebia euchroma (Royle) Johnston using rapid preparative HPLC*

Sharma

Malik

et al. 2008

J of Separation Science

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“…The antioxidant activity of shikonin and its ester derivatives isolated from Lithospermum erythrorhizon raw extract was shown in lard, alone or in combination with Ve, BHT and citric acid (Weng et al, 2000). A/S and its parent moiety, naphthazarin, competed with DMSO for free OH• very efficiently and it was indirectly proposed that some of the claimed properties for A/S, such as antiinflammatory and wound healing activity may be attributed, at least in part, to their ability to interfere with free radical processes (Kourounakis et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Radical scavenging activity of Alkanna tinctoria root extracts and their main constituents, hydroxynaphthoquinones

Assimopoulou

Papageorgiou

2005

Phytotherapy Research

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Alkannin and shikonin (A[sol ]S) are pharmaceutical substances with a wide spectrum of biological properties. Radical scavenging activity is involved in aging processes, antiinssammatory, anticancer and wound healing activities. Hence, in the present study the DPPH radical scavenging activity of alkannin and shikonin, both monomeric and oligomeric, and extracts of Alkanna tinctoria roots were studied and a structure-activity relationship was approximated. It was shown that both monomeric and oligomeric alkannin and shikonin and also A[sol ]S esters exhibited extremely high radical scavenging activity. The presence of the naphthoquinone moiety seems to be essential for that activity, while the side chain of A[sol ]S possibly plays a minor role. Esterification of A[sol ]S on the side chain hydroxyl group does not affect radical scavenging activity. Organic solvents and olive oil (extracted at room temperature) extracts of Alkanna tinctoria roots, which contain as active ingredients A[sol ]S esters, exhibited very good antiradical activity. Alkannin and shikonin and their esters and also extracts of Alkanna tinctoria roots could be used promisingly in pharmaceutical and cosmetic preparations for their radical scavenging activity and probably for their antiaging activity.

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“…The SK component can be characterized with three ultraviolet-visible absorption peaks, which are approximately at 485, 518 and 574 nm [17,18]. In this study, the absorbance at 518 nm and the corresponding calibration curve were used to measure the content of SK.…”

Section: Purity and In Vitro Cell Viability Of Skmentioning

confidence: 99%

Polyvinyl Alcohol/Lithospermum Erythrorhizon Nanofibrous Membrane: Characterizations, In Vitro Drug Release, and Cell Viability

Lou

Lee

et al. 2017

Applied Sciences

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Abstract:This study proposes an optimization process of the Lithospermum erythrorhizon (LE) extraction with a higher purity of shikonin (SK). The influence of extraction temperature on the concentration of SK is examined, and an in vitro cell viability assay is used to examine the optimal concentration of SK. Afterwards, polyvinyl alcohol (PVA)/LE solutions at ratios of 90/10, 80/20, and 70/30 w/w are electrospun into LE electrospun nanofibrous membranes (LENMs). The optimal manufacture parameters of LENMs are evaluated based on the test results of in vitro drug release test and cell viability assay. The optimal concentration occurs when the extraction temperature is −10 • C. The purity of the LE extract reaches 53.8% and the concentration of SK is 1.07 mg/mL. Moreover, the cell viability of nanofibrous membranes significantly increases to 136.8% when 0.7 µM SK is used. The diameter of nanofibers of LENM is decreased by 43.9% when the ratio of PVA solution to LE extract is 70/30 (w/w). 80/20 (w/w) LENM has the maximum amount of drug release of 79% for a continuous period of 48 h. In particular, 90/10 (w/w) LENM can create the maximum cell proliferation of 157.5% in a 24-h in vitro cell viability assay. This suggests that LENM has great potential to be used in facilitating tissue regeneration and wound healing.

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Antioxidant properties of components extracted from puccoon (Lithospermum erythrorhizon Sieb. et Zucc.)

Cited by 31 publications

References 3 publications

gIsolation and purification of acetylshikonin and β‐acetoxyisovalerylshikonin from cell suspension cultures of Arnebia euchroma (Royle) Johnston using rapid preparative HPLC*

gIsolation and purification of acetylshikonin and β‐acetoxyisovalerylshikonin from cell suspension cultures of Arnebia euchroma (Royle) Johnston using rapid preparative HPLC*

Radical scavenging activity of Alkanna tinctoria root extracts and their main constituents, hydroxynaphthoquinones

Polyvinyl Alcohol/Lithospermum Erythrorhizon Nanofibrous Membrane: Characterizations, In Vitro Drug Release, and Cell Viability

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