Antioxidant properties of glutamine and its role in VEGF-Akt pathways in portal hypertension gastropathy

Marques, Camila Moraes; Licks, Francielli; Zattoni, Ingrid Fatima; Borges, Beatriz de Carvalho; Souza, Luiz Eduardo Rizzo de; Marroni, Cláudio Augusto; Marroni, Norma Possa

doi:10.3748/wjg.v19.i28.4464

Cited by 19 publications

(17 citation statements)

References 50 publications

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“…This increase was of 476% (24h) and 646% (48h) in relation to control groups, while Vitamin E-supplemented groups showed a reduction of 36.2% (24h) and 29.8% (48h) as compared to groups receiving TAA, which in a way can be explained by the improvement of the hepatic parenchyma. Similar findings were reported by other authors who assessed nitric oxide levels (7,22) .…”

Section: Discussionsupporting

confidence: 92%

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Action of Vitamin E on Experimental Severe Acute Liver Failure

Miguel

Schemitt

Colares

et al. 2017

Arq. Gastroenterol.

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-Background -Severe Acute Liver Failure (ALF) is a life-threatening clinical syndrome characterized by hepatocyte necrosis, loss of hepatic architecture, and impairment of liver functions. One of the main causes of ALF is hepatotoxicity from chemical agents, which damage hepatocytes and result in increase of reactive oxygen species. The vitamin E isoform is the one with the strongest biological antioxidant activity. Objective -To evaluate the antioxidant effect of vitamin E in this ALF model. Methods -We used 56 rats (mean weight of 300 g) divided into eight groups, four groups assessed at 24 hours and 4 assessed at 48 hours after induction: control group (CO); Vitamin E (Vit. E); Thioacetamide (TAA) and Thioacetamide + Vitamina E (TAA+Vit.E). Rats were submitted to injections of thioacetamide (400 mg/kg i.p.) at baseline and 8 hours later. Vitamin E (100 mg/kg ip) was administered 30 minutes after the second dose of thioacetamide. The 48-hour group rats received two additional doses of vitamin E (24h and 36h). At 24h or 48 hours after the administration of the first dose of TAA, rats were weighed and anesthetized and their blood sampled for evaluation of liver integrity through enzymes aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Liver tissue was sampled for assessment of lipid peroxidation (LPO) by the technique TBARS, antioxidant enzymes SOD, CAT, GPx and GST activity, levels of the NO 2 /NO 3 and histology by H&E in two times. The results were expressed as mean ± standard deviation and statistically analyzed by ANOVA followed by Student-Newman-Keuls, with P<0.05 considered as significant. The histopathological evaluation showed a decrease in liver injury (necrosis and inflammation) in both studied times. Conclusion -These results suggest that vitamin E was able to protect the liver from lesions caused by thioacetamide. HEADINGS -Acute liver failure. Thioacetamide. Oxidative stress. Antioxidants.

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Section: Discussionsupporting

confidence: 92%

“…In a study investigating the preventive effect of quercetin in animals with TAA-induced SALF, de Oliveira et al, showed significantly increased SOD activity from the administration of the flavonoid. Other works have reported decreased SOD activity from the administration of other antioxidants (8,22,23) .…”

Section: Discussionmentioning

confidence: 81%

Action of Vitamin E on Experimental Severe Acute Liver Failure

Miguel

Schemitt

Colares

et al. 2017

Arq. Gastroenterol.

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“…Blockage of VEGF promoted portal pressure reduction [31,32], and the inhibition of JAK2/STAT3 signalling by AG490 greatly decreased the expression of VEGF and phospho-eNOS, which contributed to the inhibition of angiogenesis and the increase of the splanchnic vascular tone. Thus, in splanchnic circulation, the decreased formation of blood vessels and increased SMAR may have decreased SMABF.…”

Section: Discussionmentioning

confidence: 98%

Inhibition of Janus kinase-2 signalling pathway ameliorates portal hypertensive syndrome in partial portal hypertensive and liver cirrhosis rats

Wang

Yin

Dong

et al. 2015

Digestive and Liver Disease

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“…Gly present in the purified peptide is known to be a direct proton donor which is critical to the free radical scavenging property of antioxidant peptides due to their capacity to reduce unpaired electrons or free radicals by donating protons (Qian et al 2008). Glutamine was shown to act as a potent antioxidant that protected the gastric mucosa and decreased oxidative damage to the gastric mucosa in a rat model of portal hypertension (Marques et al 2013). Arginine supplementation enhanced antioxidant defence in experimental rats (Shan et al 2013).…”

Section: Purification Of Uf Fraction Using Semi-preparative Rp-hplcmentioning

confidence: 97%

Sequence Determination of an Antioxidant Peptide Obtained by Enzymatic Hydrolysis of Oyster Crassostrea madrasensis (Preston)

Asha

Kumari

Kumar

et al. 2016

Int J Pept Res Ther

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Soft tissue from cultured farm fresh oysters (Crassostrea madrasensis) was subjected to two standard enzymatic peptide extraction procedures using pepsin and papain. The crude extracts obtained were partially purified by column chromatography and were freeze-dried. The hydrolysates obtained were compared with respect to their degree of hydrolysis (DH), antioxidant potential (AP) and total phenolic content (TPC). The hydrolysate showing better antioxidant property was further subjected to purification by high performance liquid chromatography and characterized by LC-MS/MS. Papain-digested oyster protein (OPHpap) hydrolysate showed higher DH, AP and TPC. OPHpap was further subjected to ultrafiltration and fractionated into 3 sizes namely, above 10, 3-10 and 1-3 kDa according to the molecular size. Antioxidant capacity of\3 kDa fraction OPHpap-3 evaluated by DPPH free radical scavenging assay, metal chelating activity, linoleic acid autoxidation assay showed maximum effectiveness. Of the seven fractions collected by purification of OPH-pap-3 on semi-preparative RP-HPLC, fraction 7 that showed the highest antioxidant activity was further characterized by LC-ESI-MS/MS and its sequence determined. An antioxidant peptide molecule with thirteen amino acids was identified in oyster protein hydrolysate obtained by papain digestion that may find application as a nutraceutical or may be utilized in food industry for prevention of rancidity in foods.

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Antioxidant properties of glutamine and its role in VEGF-Akt pathways in portal hypertension gastropathy

Abstract: Glutamine treatment demonstrated to reduce oxidative damage but does not reduce angiogenesis induced by PH in gastric tissue, demonstrating a beneficial role for the PI3K-Akt-eNOS pathway.

Cited by 19 publications

References 50 publications

Action of Vitamin E on Experimental Severe Acute Liver Failure

Action of Vitamin E on Experimental Severe Acute Liver Failure

Inhibition of Janus kinase-2 signalling pathway ameliorates portal hypertensive syndrome in partial portal hypertensive and liver cirrhosis rats

Sequence Determination of an Antioxidant Peptide Obtained by Enzymatic Hydrolysis of Oyster Crassostrea madrasensis (Preston)

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