Antioxidant Properties of Hydrogen Gas Attenuates Oxidative Stress in Airway Epithelial Cells

Abstract

Oxidative stress plays a crucial role in the development of airway diseases. Recently, hydrogen (H2) gas has been explored for its antioxidant properties. This study investigated the role of H2 gas in oxidative stress-induced alveolar and bronchial airway injury, where A549 and NCI-H292 cells were stimulated with hydrogen peroxide (H2O2) and lipopolysaccharide (LPS) in vitro. Results show that time-dependent administration of 2% H2 gas recovered the cells from oxidative stress. Various indicators including reactive oxygen species (ROS), nitric oxide (NO), antioxidant enzymes (catalase, glutathione peroxidase), intracellular calcium, and mitogen-activated protein kinase (MAPK) signaling pathway were examined to analyze the redox profile. The viability of A549 and NCI-H292 cells and the activity of antioxidant enzymes were reduced following induction by H2O2 and LPS but were later recovered using H2 gas. Additionally, the levels of oxidative stress markers, including ROS and NO, were elevated upon induction but were attenuated after treatment with H2 gas. Furthermore, H2 gas suppressed oxidative stress-induced MAPK activation and maintained calcium homeostasis. This study suggests that H2 gas can rescue airway epithelial cells from H2O2 and LPS-induced oxidative stress and may be a potential intervention for airway diseases.

High Concentration Hydrogen Mitigates Sepsis-Induced Acute Lung Injury in Mice by Alleviating Mitochondrial Fission and Dysfunction

“…Hydrogen has significant therapeutic effects on the dysfunction of organs such as intestine, kidney, liver, lung, myocardium and brain. Not only that, it also has definite therapeutic effect on type 2 diabetes, multiple organ injury, sepsis, atherosclerosis, tumor and many other diseases [ 19 , 20 , 21 , 22 , 23 , 24 ]. At present, we know that hydrogen is insoluble in water.…”

Discovery of Novel HSP27 Inhibitors as Prospective Anti-Cancer Agents Utilizing Computer-Assisted Therapeutic Discovery Approaches

“…It inhibits ERK through competition with ATP (Type I ATP inhibitor) [ 38 , 39 ]. FR180204 has been shown to have an antiproliferative effect in different cancer cell lines, including adrenocortical, mesothelioma, prostate, pancreatic, and colorectal cell lines [ 40 , 41 ]. Similarly, XAV-939 has been shown to target the Wnt/B-catenin signaling pathway by inhibiting tankyrases 1 and 2.…”

Anti-Hyperglycemic Effect of Magnesium-Enhanced Alkaline-Reduced Water on High Glucose-Induced Oxidative Stress in Renal Tubular Epithelial Cells

“…In addition, MARW enhanced antioxidant activities against HG-induced OS, which supports our ROS and NO results. Antioxidant enzymes, such as GPx and CAT, can help cells to recover from the effects of OS [32][33][34]. These antioxidants are well known for their involvement in lowering OS effectors such as ROS and NO levels in HK-2 cell lines [32,35].…”

“…p-p38 and p-JNK are downstream proteins of the MAPK pathway and play important roles in early OS and cellular responses [43,44]. Previously published studies have confirmed that, with blocking, the MAPK signaling pathway can aid in the survival of cells in a redox imbalance state [34,45,46]. However, it has been explained that ARW increases endogenous antioxidants that help to protect cells from OS [32].…”