Antioxidant properties of Komagataeibacter hansenii CGMCC 3917 and its ameliorative effects on alcohol-induced liver injury in mice

Abstract

This study was to investigate the antioxidant activity of Komagataeibacter hansenii CGMCC 3917 in vivo and in vitro. In vitro study suggested that CGMCC 3917 exhibited strong scavenging abilities against DPPH, ABTS, OH free radicals and reducing power, suggesting significant anti-oxidant ability. In vivo supplementation of mice with CGMCC 3917 bacteria suspension at a dose of 10 mL/kg (10 8 or 10 6 CFUs/mL) dramatically decreased the elevated serum activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) induced by alcohol treatment in mice (p< 0.01), and also significantly decreased the elevated levels of alkaline phosphatase (ALP) and hepatic malondialdehyde (MDA) induced by alcohol treatment in mice (p< 0.01). Furthermore, CGMCC 3917 supplement showed the preventive effects against the alcohol-induced elevation in serum triglycerides (TG) and low-density lipoprotein cholesterol (LDL-C), and the decrease in high-density lipoprotein cholesterol (HDL-C), as well as the decreased activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in mice. Additionally, supplementation with CGMCC 3917 displayed significant anti-inflammatory effects by lowering hepatic production of TNF-α, IL-1, and IL-6 in alcohol-treated mice. All these findings suggest that K. hansenii CGMCC 3917 may be a great potential nutritional resource against chronic alcohol-induced liver oxidative injury in mice. Propiedades antioxidantes de Komagataeibacter hansenii CGMCC 3917 y sus efectos de mejora en lesiones hepáticas de ratones inducidas por alcohol RESUMEN El presente estudio se propuso investigar la actividad antioxidante de Komagataeibacter hansenii CGMCC 3917 in vivo e in vitro. El estudio in vitro indicó que CGMCC 3917 exhibe una fuerte capacidad de eliminación de radicales DPPH, ABTS y OH, además de poder reductor. Ello sugiere que posee una significativa capacidad antioxidante. La suplementación in vivo de ratones tratados con alcohol con suspensión de bacterias CGMCC 3917 a una dosis de 10 ml/kg (10 8 o 10 6 UFC/ml) disminuyó drásticamente la elevada actividad sérica de la aspartato aminotransferasa (AST) y la alanina aminotransferasa (ALT) inducida por el tratamiento con alcohol (p<0.01). Además, redujo significativamente los niveles elevados de fosfatasa alcalina (ALP) y malondialdehído hepático (MDA) inducidos por el tratamiento con alcohol (p<0.01). Por otra parte, el suplemento CGMCC 3917 administrado a ratones mostró efectos preventivos de la elevación de triglicéridos (TG) en el suero, así como del colesterol de lipoproteínas de baja densidad (LDL-C), y de la disminución del colesterol de lipoproteínas de alta densidad (HDL-C), y de las actividades de la glutatión peroxidasa (GSH-Px) y la superóxido dismutasa (SOD) inducidas por el alcohol. Además, la suplementación con CGMCC 3917 mostró efectos antiinflamatorios significativos al disminuir la producción hepática de TNF-α, IL-1 e IL-6 en ratones tratados con alcohol. Estos hallazgos sugieren que K. hansenii CGMCC 3917 puede ser potenc...

The Role of Oxidative Stress in Alcoholic Fatty Liver Disease: A Systematic Review and Meta-Analysis of Preclinical Studies

“…After this analysis, 223 articles were potentially eligible for the review; however, 17 were excluded owing to missing data. Therefore, 206 files [ 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , …”

“…A total of 206 [ 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 ,…”

Life History Traits in Two Drosophila Species Differently Affected by Microbiota Diversity under Lead Exposure

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“…The hepatic levels of malondialdehyde (MDA) and glutathione (GSH) are considered as typical indexes of antioxidant status of tissues (Jiang et al, 2019). GSH is the major non-protein thiol that plays a vital role in maintaining the antioxidant defense mechanism in the body (Guldas et al, 2021).…”