Antioxidant Status and Lipid Peroxidation in Diabetic Rats under Hyperbaric Oxygen Exposure

Abstract

Hyperglycemia is known to cause oxidative stress that leads mainly to enhanced production of mitochondrial reactive oxygen species (ROS). It has been demonstrated that hyperbaric oxygen (HBO) treatment also increases the formation of ROS. There are, however, no comprehensive evaluations of such oxidative effects in diabetes which requires HBO treatment. The purpose of this study is to investigate the influence of a clinically-recommended HBO treatment on glucose homeostasis and oxidative stress in rats with streptozotocin (STZ)-induced diabetes. Under the clinically-used HBO exposure protocol, the levels of blood glucose, thiobarbituric acid reactive substances (TBARS) as a lipid peroxidation marker, and the activity of superoxide dismutase (SOD) as an antioxidant enzyme marker were investigated in the erythrocytes, liver, pancreas, skeletal muscle, and brain of rats with STZ-induced diabetes. The levels of blood glucose and TBARS increased significantly (p<0.05), and the activity of SOD decreased significantly (p<0.05) in the erythrocytes and all organs of rats with diabetes subjected to HBO exposure. These results suggested that HBO exposure might boost glucose autoxidation and increase ROS production in STZ-induced diabetes as side-effects of administering HBO treatment for the first time.

Hyperbaric Oxygen Therapy Ameliorates the Harmful Effects of Dexamethasone Induced Diabetes on Liver and Pancreas of Adult Male Albino Rats

“…In this work, no significant differences in blood glucose were noticed between control and HBO treated non diabetic groups in all experimental periods. Similar findings were reported in previous work [22]. A highly significant increase in blood glucose was noticed after 10 days injection of dexamethasone in group III and IV compared to the control rats.…”

“…This may be explained by catabolic processes, hypoinsulinemia that led to increased appetite owing to sense of energy deficiency caused by disturbed glucose transport [27]. At the end of this study, the body weight of the HBO treated diabetic rats (group IV) showed highly significant decrease compared with the rats of group I. similar findings were demonstrated previously [22]. It was suggested that weight loss enhanced insulin sensitivity [28] and this is one of the mechanisms by which HBOT is effective in treatment of type 2 diabetes [25].…”

Leaf Extracts of Anchomanes difformis Ameliorated Kidney and Pancreatic Damage in Type 2 Diabetes

“…ORAC assay was carried out following the method of Prior and colleagues [60], while FRAP was determined using the method described by Benzie and Strain [61]. Lipid peroxidation was measured to assess oxidative stress status in the kidney by determining TBARS levels in the kidney homogenates using the combined methods of Matsunami et al and Wasowicz et al [62,63].…”

Delaying of cataract through intervention ofHemidesmus indicusin STZ induced diabetic rats

“…The level of thiobarbituric reactive substance (TBARS) in the lens homogenate has been analyzed according to the method described by Ohkawa et al 1979(Ohkawa et al 1979) and Matsunami et al 2010(Matsunami et al 2010. The absorption of supernatant was measured spectrophotometrically at 535 nm.…”