Antioxidant Status, Antidiabetic Properties and Effects on Caco-2 Cells of Colored and Non-Colored Enriched Extracts of Sweet Cherry Fruits

Gonçalves, Ana C.; Rodrigues, Márcio; Santos, Adriana O.; Falcão, Amı́lcar; Silva, Luís

doi:10.3390/nu10111688

Cited by 52 publications

(172 citation statements)

References 51 publications

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“…Among flavonols, only 3 derivatives were identified: Kaempferol-3-rutinoside, kaempferol-3-glucoside, and quercetin derivative, which were reported previously by Martini, Conte, and Tagliazucchi [5] in exotic varieties and Portuguese varieties, for instance, Saco sweet cherry for Gonçalves, Bento, Silva, and Silva [47], and in Saco cherry polyphenol-rich extracts [8,17]. Regarding anthocyanins, a total of 5 were identified in antioxidant polyphenol-rich extracts: Cyanidin-3-glucoside, cyanidin-3-rutinoside, peonidin-3-rutinoside, peonidin-3-glucoside, and pelargonidin-3-glucoside.…”

Section: Identification Of Major Phenolic Compounds By Lc-esi-qqtof-hrmssupporting

confidence: 75%

“…Regarding anthocyanins, a total of 5 were identified in antioxidant polyphenol-rich extracts: Cyanidin-3-glucoside, cyanidin-3-rutinoside, peonidin-3-rutinoside, peonidin-3-glucoside, and pelargonidin-3-glucoside. These anthocyanins had been already identified in the Saco variety [47] and in polyphenol-rich extracts produced from Saco cherry [17], being that these anthocyanins are the most reported in cherries, in spite of the variety. All the extraction methodologies showed to be able to recover all phenolic compounds except on MAE extracts, where three of the hydroxycinnamic acids were not detected.…”

Section: Identification Of Major Phenolic Compounds By Lc-esi-qqtof-hrmsmentioning

confidence: 86%

“…On the other hand, Martini, Conte, and Tagliazucchi [5] identified 12 more flavanols in cherry exotic varieties, including procyanidins, and Serra, Duarte, Bronze, and Duarte [4] identified two flavanols in Saco cherries ((+)-catechin and (−)-epicatechin). Furthermore, Matias, Rosado-Ramos, Nunes, Figueira, Serra, Bronze, Santos, and Duarte [8] reported the presence of (+)-catechin and procyanidin B2 in antioxidants extracts from Saco cherry produced by recovery (poly)phenols from extracts by adsorption process, although, Gonçalves, Rodrigues, Santos, Alves, and Silva [17] did not identify any flavanols in Saco cherry hydro-alcoholic extracts.…”

Section: Identification Of Major Phenolic Compounds By Lc-esi-qqtof-hrmsmentioning

confidence: 98%

“…Since one of the main purposes of this work was to produce extracts, by the application of four different methodologies, following the evaluation of their bioactivities, the variables regarding type of solvent and ratio were fixed. For that, a mixture of water:ethanol (50:50, v/v) was selected, because (i) these are food grade solvents, and thus are considered to be safe for the consumer even if not completely removed from the final ingredient, (ii) are environmentally friendly, (iii) could be reused in the process, and finally, (iv) this mixture is one of the most common mixtures of solvents used to produce extracts rich in BCs [17]. The extraction yield of the different Saco cherry antioxidant polyphenol-rich extracts obtained by different extraction techniques are shown in Figure 1.…”

Section: Extractive Yieldmentioning

confidence: 99%

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Polyphenol Extraction by Different Techniques for Valorisation of Non-Compliant Portuguese Sweet Cherries towards a Novel Antioxidant Extract

et al. 2020

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Currently, there is special interest in the recovery of polyphenols from non-compliant fruits that have no market value; efforts to find value-added solutions for these food areas are a key option for a sustainable bio-economy. Saco cherries are a traditional Portuguese cherry variety, and although they are a nutritionally important food, rich in powerful dietary polyphenols, significant amounts of these cherries are not sold due to their small size. In this context, this work aimed to select the best method to produce novel antioxidant polyphenol-rich extracts from low calibre and non-compliant Saco cherries. Based on the results, microwaves-assisted extraction (MAE) allowed us to obtain a polyphenol-rich extract with a high antioxidant capacity (50.46 ± 1.58 mg Trolox equivalent (TE)/g dry extract (DE) by oxygen radical absorbance capacity (ORAC), 10.88 ± 0.38 mg ascorbic acid equivalent (AA)/g DE by 2-azinobis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS), and 9.58 ± 0.42 mg TE/g DE by 2,2-diphenyl-1-picrylhydrazyl (DPPH)) and a high content of polyphenols, namely, hydroxycinnamic acids (neochlorogenic and p-coumaric acids) and anthocyanins (cyanidin-3-rutinoside and cyanidin-3-glucoside), compared with those of conventional extractions with low and high temperature and ultrasound-assisted extraction. The antioxidant extract produced from MAE could be a new alternative for the valorisation of non-compliant cherries since these extracts proved to be a functional ingredient due to the high content of antioxidants, which are linked to the prevention of diseases.

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Section: Identification Of Major Phenolic Compounds By Lc-esi-qqtof-hrmssupporting

confidence: 75%

Section: Identification Of Major Phenolic Compounds By Lc-esi-qqtof-hrmsmentioning

confidence: 86%

Section: Identification Of Major Phenolic Compounds By Lc-esi-qqtof-hrmsmentioning

confidence: 98%

Section: Extractive Yieldmentioning

confidence: 99%

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Polyphenol Extraction by Different Techniques for Valorisation of Non-Compliant Portuguese Sweet Cherries towards a Novel Antioxidant Extract

et al. 2020

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“…Specifically, previous studies have proven the ability of phenolic-rich ingredients (berry extracts, soft-fruits, grape seeds and bitter melon to inhibit the activities of pancreatic α-amylase and intestinal α-glucosidase in the gut lumen [ 51 , 52 ]. Other studies have shown that anthocyanin-rich fractions from blueberry [ 53 ], blackberry [ 54 ], black legumes [ 55 ], black mulberry [ 56 ], bilberry and cranberry [ 57 ], red aril fruits [ 58 ], camelina and ophia seeds [ 59 ], royle fruits [ 60 ], fruits of Chilean berberis species [ 61 ], peach fruit and A. melanocarpa fruit juice extracts [ 62 , 63 ], colored extracts of Saco sweet cherry [ 64 ], and anthocyanin extracts from black bean hull, black currants and black rice [ 65 , 66 ] exhibited higher effectiveness to inhibit α-glucosidase. Therefore, diet-induced treatments from anthocyanin-based extracts, often combined with other polyphenols, may offer a natural alternative to achieve better glycemic control in T2DM.…”

Section: Why Anthocyanins?mentioning

confidence: 99%

Anthocyanins as Antidiabetic Agents—In Vitro and In Silico Approaches of Preventive and Therapeutic Effects

et al. 2020

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Many efforts have been made in the past two decades into the search for novel natural and less-toxic anti-diabetic agents. Some clinical trials have assigned this ability to anthocyanins, although different factors like the food source, the amount ingested, the matrix effect and the time of consumption (before or after a meal) seem to result in contradictory conclusions. The possible mechanisms involved in these preventive or therapeutic effects will be discussed—giving emphasis to the latest in vitro and in silico approaches. Therapeutic strategies to counteract metabolic alterations related to hyperglycemia and Type 2 Diabetes Mellitus (T2DM) may include: (a) Inhibition of carbohydrate-metabolizing enzymes; (b) reduction of glucose transporters expression or activity; (c) inhibition of glycogenolysis and (d) modulation of gut microbiota by anthocyanin breakdown products. These strategies may be achieved through administration of individual anthocyanins or by functional foods containing complexes of anthocyanin:carbohydrate:protein.

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Antioxidant, anti‐inflammatory, and anticancer function of Engleromyces goetzei Henn aqueous extract on human intestinal Caco‐2 cells treated with t‐BHP

Cheng

Yu‐Ting

et al. 2023

Food Science & Nutrition

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High body mass index (high BMI, obesity) is a serious public health problem, and “obesity‐induced oxidative stress, inflammation, and cancer” have become modern epidemic diseases. We carried out this study to explore a functional beverage that may protect against obesity‐induced diseases. The Engleromyces goetzei Henn herbal tea is such a candidate. For this study, we carried out LC–MS analysis of E. goetzei Henn aqueous extract (EgH‐AE); then used the Caco‐2 cell line for the model cells and treated the cells with t‐BHP to form an oxidative stress system. An MTT assay was used for testing the biocompatibility and cytoprotective effects; reactive oxygen species and malondialdehyde determination was used for evaluating the antioxidative stress effect; TNF‐α and IL‐1β were used for observing the anti‐inflammatory effect, and 8‐OHdG for monitoring anticancer activity. The results of this study demonstrate that the EgH‐AE has very good biocompatibility with the Caco‐2 cell line and has good cytoprotective, antioxidant, anti‐inflammatory, and anticancer properties. It is clear that EgH‐AE, a kind of ancient herbal tea, may be used to develop a functional beverage that can be given to people with a high BMI to protect against obesity‐induced diseases.

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Antioxidant Status, Antidiabetic Properties and Effects on Caco-2 Cells of Colored and Non-Colored Enriched Extracts of Sweet Cherry Fruits

Cited by 52 publications

References 51 publications

Polyphenol Extraction by Different Techniques for Valorisation of Non-Compliant Portuguese Sweet Cherries towards a Novel Antioxidant Extract

Polyphenol Extraction by Different Techniques for Valorisation of Non-Compliant Portuguese Sweet Cherries towards a Novel Antioxidant Extract

Anthocyanins as Antidiabetic Agents—In Vitro and In Silico Approaches of Preventive and Therapeutic Effects

Antioxidant, anti‐inflammatory, and anticancer function of Engleromyces goetzei Henn aqueous extract on human intestinal Caco‐2 cells treated with t‐BHP

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