Antioxidant Status in STZ-induced diabetic rats treated with Vanadium pentoxide nanoparticles

Abstract

Antioxidant status determines the susceptibility of tissues to the oxidative stress associated with diabetes and its complications; hence in the present study antioxidant status was explored in streptozotocin-induced diabetic rats treated with vanadium in the form of vanadium pentoxide nanoparticles. Vanadium pentoxide and vanadium pentoxide nanoparticles at the dose rate of 5mg/kg were administered orally in STZ (50mg/Kg) induced diabetic rats for 30 days and glimepiride (reference drug) was administered orally at the dose rate of 800 ìg/kg body weight. Vanadium pentoxide nanoparticles significantly reduced the blood glucose levels than the diabetic control and other treatment group of rats. On exploration of antioxidant status in liver, kidney and pancreas tissues, vanadium in the form of vanadium pentoxide nanoparticles outperformed the vanadium pentoxide by increasing the activities of the enzymes catalase, superoxide dismutase and glutathione peroxidase, and the concentration of reduced glutathione and by decreasing the lipid peroxide levels. The present study also showed that the restoration of antioxidant status by vanadium pentoxide nanoparticles is comparable with that of the reference drug. It can be concluded that vanadium pentoxide nanoparticles, due to its superior control over hyperglycemia and antioxidant properties, outperformed the vanadium pentoxide treatment in enhancing the antioxidant status in diabetic rats.

Vanadium and biomarkers of inflammation and oxidative stress in diabetes: A systematic review of animal studies

“…The studies’ publication dates ranged from August 1990 37 to November 2021. 38 In 24 out of the included studies, 12 , 16 - 18 , 20 , 21 , 39 - 56 randomization was reported. Sample size was from 20 57 to 90 2 animals.…”

“…Additionally, 3 studies 2 , 16 , 43 were on mice species. Rat’s mean weight was 175 g and mice’s mean weight was 26.5 g. Sources of vanadium consumed for administration were vanadyl sulfate (20 studies), 2 , 12 , 17 , 18 , 21 , 39 - 42 , 44 , 48 - 51 , 53 , 54 , 57 , 58 , 60 sodium orthovanadate (10 studies), 6 , 8 , 11 , 19 , 22 , 37 , 46 , 62 - 64 oxovanadium (IV) complex (1 study), 2 vanadium pentoxide (2 studies), 20 Na[(O2)2(2,2’-bpy)] • 8 H2O vanadium complexes (1 study), 13 vanadyl(IV)-ascorbate (VOAsc) complex (1 study), 43 dioxidovanadium cis-[VO2(obz)py]) complex (1 study), 38 NaVO3 (3 studies), 21 , 47 vanadium-3-hydroxy flavone complex (1 study), 59 macrocyclic binuclear oxovanadium (IV) complex (MBOV) (1 study), 14 bis(maltolato)oxovanadium (IV) (2 studies), 16 , 45 dioxidovanadium (1 study), 61 vanadium citrate (1 study), 24 V3dipic-Cl 21 and oxovanadium(IV) chelate [VOL] (1 study). 51 Method of administration was either by dissolving into drinking water, 6 , 8 , 11 , 16 , 17 , 19 , 21 , 22 , 24 , 37 , 40 , 41 , 44 - 47 , 57 - …”

“…Randomization was reported in 24 of the included studies. 12 , 16 - 18 , 20 , 21 , 39 - 56 Animals were similar in age and weight and were kept in controlled and similar conditions. Figure 2 illustrates the methodological quality assessment of studies and Figure 3 shows the risk of bias of each item, as percentages.…”

“… 2 , 21 , 41 , 42 , 44 , 48 , 50 , 53 , 54 , 60 Other compounds such as oxovanadium (IV) complex, sodium orthovanadate, [VO(SO4)(1,10’-phen)] • 2 H2O, [VO(SO4)(2,2’-bpy)] • H2O, vanadyl(IV)-ascorbate, Dioxidovanadium (V) complex, vanadium-3-hydroxy flavone complex, macrocyclic binuclear oxovanadium (IV) complex, bis(maltolato)oxovanadium IV (BMOV), vanadium citrate, vanadium pentoxide also enhanced SOD level in different tissues of animals. 2 , 6 , 8 , 13 , 14 , 16 , 19 , 20 , 22 , 24 , 37 , 38 , 43 , 46 , 51 , 59 , 63 …”

“… 7 Vanadium, as a transitional metal with redox properties, enters the redox process, produces free radicals, and distracts the pro-antioxidant balance in the cell. Vanadium is a scavenger of superoxide radicals, and declines antioxidant enzymes such as SOD, GPx, CAT, and GR in erythrocytes, 8 - 11 liver, 12 - 22 kidneys, 14 , 19 , 20 , 22 heart, 22 brain, 22 , 23 pancreas 16 , 20 , 24 and testes 25 of rats. Based on the results of multiple studies, vanadium complexes increase the action of GPx and demolish the effect of ROS in diabetic-induced rats.…”