Antioxidant status of lambs fed on diets supplemented with selenite or Se-yeast

Abstract

The effects of feed supplementation with inorganic and organic forms of selenium (Se) on the activities of blood glutathione peroxidase (GPx), superoxide dismutase (SOD) in erythrocytes as well as on total antioxidant status in plasma (TAS) were examined in lambs. Fifteen animals were divided into three groups and fed experimental diets for 3 months. The first group received the basic diet (BD) providing a daily intake 50.6 μg of Se only. Diets for the second and third groups consisted of BD supplemented with selenium 0.3 mg . kg -1 of DM in the form of sodium selenite or Se-enriched yeast and giving a total daily intake 278.6 μg of Se per animal. Increases in Se concentrations in whole blood, plasma and red blood cells as well as in the activities of blood GPx were highly significant in both supplemented groups, but no differences could be found that were due to the form of Se administered. The activity of SOD in erythrocytes was found to be significantly higher in the selenite group of lambs compared with both groups given BD or Se-yeast. The total antioxidant status was significantly lower in the group given Se-yeast compared with animals on BD, which might be explained by larger amounts of selenide continuously produced from body Se deposits. The presented results support the opinion on the pro-oxidative features of sodium selenite when it is utilized as an animal feedstuff additive.

Dietary selenite and conjugated linoleic acid isomers influence fatty acid concentrations in the liver and femoral muscles of rats

“…Currently, about thirty Se-proteins have been indentified in mammals, but the physiological function of only less than half of them is well known. Among them, the best understood Se-Cys-protein appears to be cytosolic glutathione peroxidase, which functions as an antioxidant by removing reactive oxygen substances (Boldižárová et al, 2005). In agreement with the above, our recent studies revealed that dietary organic-Se or inorganicSe (Czauderna et al, 2004a) stimulated the accumulation of unsaturated fatty acids (UFA), conjugated linoleic acid (CLA) isomers in particular.…”

Comparison of organic and inorganic forms of selenium in the mother and kid relationship in goats

“…On the basis of our results we may conclude that all forms of selenium applied increased the level of selenium in the blood of mothers and in the blood of kids, but we did not discover significant differences between the individual forms of selenium. Other authors also came to the conclusion that both the inorganic and organic form of selenium increased the selenium concentrations in the blood of lambs (Boldižárová et al, 2005), dairy cows (Juniper et al, 2006), and goats (Misurova et al, 2009a) in contrast to the control group. Leeson et al (2008) compared selenite, Se-enriched yeast, and B-Traxim Se in broilers and discovered that plasma concentrations of selenium did not differ significantly when using various forms of selenium.…”

Selenite, selenized yeast, or conjugated linoleic acid isomers supplemented to the diet influence the fatty acid profile in the spleen and blood plasma of rats

“…Considering above, we suggest that dietary SeIV, especially H SeIV, has a pro-oxidant effect on the CLA isomers in the spleen. The pro-oxidative properties of sodium selenite are known from in vitro and in vivo experiments (Spallholz, 1997;Terada et al, 1999;Boldižárová et al, 2005). Consequently, the combined addition of CLAmix and H SeY to the diet showed a high but not significant increase of the concentration of c9t11CLA in the spleen (+27%) and blood plasma (+21%).…”

“…0.5 ppm Se as SeY) exhibited some pro-oxidative. It has been suggested that larger doses of dietary selenized yeast may lead to higher continuous release of selenomethionine from body deposits into the free amino acid pool with a subsequently higher production of H 2 Se, which has pro-oxidative properties (Boldižárová et al, 2005). PUFA mg/g n-6/n-3…”