Antioxidant treatment ameliorates germ cell apoptosis induced by a high-dose ionizing irradiation in rats

Sukhotnik, Igor; Nativ, Ofer; Ben-Shahar, Yoav; Bejar, Ilan; Pollak, Yulia; Coran, Arnold G.; Gorenberg, Miguel

doi:10.1007/s00383-018-4385-3

Cited by 11 publications

(5 citation statements)

References 24 publications

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“…Testicular radiation injury is the major factor in reducing fertility in cancer patients [ 4 ]. The molecular pathways that control spermatogenesis under radiation and the mechanisms of DNA damage response and homeostasis in spermatogonal stem cells remain unclear [ 5 ]. Spermatogonial stem cells support the differentiation of normal germ cell and must be preserved throughout life [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Sulforaphane attenuates irradiation induced testis injury in mice

Ran,

Duan,

Gao

et al. 2023

Redox Report

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Objective: The testis is vulnerable to ionizing radiation, sexual dysfunction and male infertility are common problems after local radiation or whole-body exposure. Currently, there are no approved drugs for the prevention or treatment of radiation testicular injury. Sulforaphane (SFN) is an indirect antioxidant that induces phase II detoxification enzymes and antioxidant genes. Herein, we investigated the radiation protective effect of SFN on testicular injury in mice and its potential mechanism. Materials and Methods: Mice were randomly divided into blank control group (Ctrl), radiation + no pretreatment group (IR), and radiation + SFN groups (IRS). In the radiation + SFN groups, starting from 72 h before radiation, SFN solution was intraperitoneally injected once a day until they were sacrificed. Mice in the blank control group and the radiation + no pretreatment group were simultaneously injected intraperitoneally with an equal volume of the solvent used to dissolve SFN (PBS with a final concentration of 0.1%DMSO) until they were sacrificed. They were subjected to 6Mev-ray radiation to the lower abdominal testis area (total dose 2Gy). Twenty-four hours after radiation, six mice in each group were randomly sacrificed. Seventy-two hours after radiation, the remaining mice were sacrificed. Results: The results showed that the harmful effects of ionizing radiation on testes were manifested as damage to histoarchitecture, increased oxidative stress, and apoptosis, and thus impaired male fertility. SFN injections can reverse these symptoms. Conclusions: The results showed that SFN can improve the damage of mouse testis caused by irradiation. Furthermore, SFN prevents spermatogenesis dysfunction caused by ionizing radiation by activating Nrf2 and its downstream antioxidant gene.

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Section: Introductionmentioning

confidence: 99%

Sulforaphane attenuates irradiation induced testis injury in mice

Ran,

Duan,

Gao

et al. 2023

Redox Report

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“…However, the results were inconsistent for the control group (without vitamin C), as there was no protection against radiation. Thus, it was concluded that the protective effect is accompanied by doses of vitamin C, as evidenced by several research groups today (Brand et al, 2015;Mortazavi et al, 2015;Paull et al, 2000;Sato et al, 2015;Sukhotnik et al, 2018).…”

Section: Comparison Between Foci In γ and X-rays (Control And Vitamin C Groups) To Detect Dsb In Dna By γ-H2axmentioning

confidence: 96%

“…New antioxidants (water-soluble molecules) have been used to decrease radiation damage (Sukhotnik et al, 2018). Small, enzymatic molecules have been tested to reduce cytotoxicity and long-term oxidative damage (Okunieff et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

EVALUATION THE ROLE OF VITAMIN C AS a RADIATION PROTECTIVE AGENT USING Γ-H2ax FOR SIGNALING OF DNA DAMAGE ON IRRADIATED MICE TESTIS

Alani¹,

Almusawi²,

Mahdi³

2020

PTQ

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An interesting feature of ionizing radiation, especially Gamma and X-rays as a DNA damaging factor is the range of lesions it induces. γ-H2AX foci are documented to represent DNA double-strand breaks (DSBs) as a biomarker for radiation-induced damage. Study design 42 adult male mice Albino BALB/c, had been divided randomly into 6 groups of seven mice each. Group 1 received a standard saline solution untreated also, do not expose to radiation. Group 2 mice received vitamin C (VC) (200 mg/kg.day) intra-peritoneal (i.p.) injected for 8 days without radiation. Group 3 control was exposed to γ-radiation. Group 4 control was exposed to X-ray radiation. Group 5 mice had been administrated with vitamin C in the identical dose of group 2 for 8 days, then exposed to (4 Gy) of γ-ray. Group 6 was administrated with vitamin dose in the same above and the same period, then exposed to (4 Gy) of X-ray. All groups had been sacrificed by cervical dislocation at (1, 3, and 24 h). Post radiation testis mice tissues were collected. A significant difference (P 0.05) between the group of vitamin C and with a control group exposed to both (γ, X-rays) in foci forming, but there is no significant difference (P 0.05) between γ and X- rays for the control and vitamin C groups. The results demonstrate that vitamin C is a good radioprotective agent for testis mice tissues; the effect of (γ and X-rays) had almost the same results on the mice testicle tissues with the same dose.

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“…Various drugs, with distinct mechanisms, have been employed to mitigate the impact of heat stress on spermatogenic cells in testicular tissue. Concurrently, the use of antioxidants to counteract these adverse effects has been explored [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Caffeine attenuates spermatogenic disorders in mice with induced chronic scrotal hyperthermia

Raoofi,

Gholami,

Mokhtari

et al. 2024

Clin Exp Reprod Med

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Objective: Chronic scrotal hyperthermia (SHT) can lead to serious disorders of the male reproductive system, with oxidative stress playing a key role in the onset of these dysfunctions. Thus, we evaluated the impact of caffeine, a potent antioxidant, on cellular and tissue disorders in mice with chronic SHT.Methods: In this experimental study, 56 adult male NMRI mice were allocated into seven equal groups. Apart from the non-treated control group, all were exposed to heat stress. Two groups, termed “preventive” and “curative,” were orally administered caffeine. The preventive mice began receiving caffeine immediately prior to heat exposure, while for the curative group, a caffeine regimen was initiated 15 consecutive days following cessation of heat exposure. Each treated group was subdivided based on pairing with a positive control (Pre/curative [Cur]+PC) or a vehicle (Pre/Cur+vehicle). Upon conclusion of the study, we assessed sperm characteristics, testosterone levels, stereological parameters, apoptosis, antioxidant and oxidant levels, and molecular markers.Results: Sperm parameters, testosterone levels, stereological parameters, biochemical factors (excluding malondialdehyde [MDA]), and c-kit gene expression were significantly elevated in the preventive and curative groups, especially the former, relative to the other groups. Conversely, expression levels of the heat shock protein 72 (HSP72) and nuclear factor kappa beta (NF-κβ) genes, MDA levels, and apoptotic cell density were markedly lower in both caffeine-treated groups relative to the other groups, with more pronounced differences observed in the preventive group. Conclusion: Overall, caffeine attenuated cellular and molecular abnormalities induced by heat stress in the testis, particularly in the mice treated under the preventive condition.

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Antioxidant treatment ameliorates germ cell apoptosis induced by a high-dose ionizing irradiation in rats

Cited by 11 publications

References 24 publications

Sulforaphane attenuates irradiation induced testis injury in mice

Sulforaphane attenuates irradiation induced testis injury in mice

EVALUATION THE ROLE OF VITAMIN C AS a RADIATION PROTECTIVE AGENT USING Γ-H2ax FOR SIGNALING OF DNA DAMAGE ON IRRADIATED MICE TESTIS

Caffeine attenuates spermatogenic disorders in mice with induced chronic scrotal hyperthermia

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