Antioxidant treatment during preservation of bovine ovaries increased the development potential of embryos

Abstract

The overnight preservation of bovine ovaries would be highly useful in the subsequent harvest of viable oocytes for reproductive study. The present study aimed to optimize conditions for overnight preservation of bovine ovaries by examining the effects of temperature, solution and supplementation. In Experiment 1, the rate of development to the blastocyst stage of oocytes derived from ovaries preserved at 15°C was higher than that at either 5 or 25°C (p < 0.05). In Experiment 2, the rate of development to the blastocyst stage of oocytes derived from ovaries preserved in University of Wisconsin solution was higher than when PBS or saline was used (p < 0.05). In Experiment 3, oocytes preserved in saline supplemented with 0.3 mM glutathione (GSH) exhibited an increase in the rate of blastocyst formation compared with oocytes supplemented with 0 or 3 mM GSH (p < 0.05). In Experiment 4, supplementation with 10 μM epigallocatechin gallate during ovary preservation increased the rate of blastocyst formation (p < 0.05). The blastocysts derived from ovaries stored in saline supplemented with GSH at 15°C for 24 h were shown to develop into normal offsprings following transfer to recipient heifers. Our studies indicate that bovine IVM/IVF embryos derived from ovaries preserved in saline supplemented with an antioxidant at 15°C for 24 h can successfully develop to the blastocyst stage and result in offspring.

The Developmental Potential of Oocytes is Impaired in Cattle with Liver Abnormalities

“…The ovaries were maintained at 15–20 C and transported to the laboratory in a saline solution supplemented with antibiotics and antimycotics (AB; Invitrogen, Carlsbad, CA, USA); the time between recovery of ovaries and transport to the laboratory was approximately 1 h. The ovaries were then held in saline supplemented with 0.3 mM reduced glutathione (GSH; G4251, Sigma-Aldrich, St. Louis, MO, USA) and 1% AB at 15 C for 20 h with shaking [17]. …”

Effect of melatonin supplementation in the long-term preservation of the sheep ovaries at different temperatures and subsequent in vitro embryo production

“…2 effective use of the oocytes contained within [6]. The type of transportation medium [3,[6][7][8], storage time [3][4][5][6][7][8][9][10][11][12][13][14][15][16], as well as, its temperature during transportation of the ovaries [4,5,[10][11][12][13][14][15][16] are among the factors affecting subsequent follicular and oocyte survival, and oocyte developmental competence [7]. Consequently, many studies have been conducted in order to improve oocyte preservation by modifying the transportation solutions.…”

“…The main goal in ovaries preservation is to maintain the function of the oocyte enclosed in follicle for in vitro maturation and fertilization. Accordingly, many studies investigated the effects of different antioxidants to reduce the damage caused by free oxygen radicals during ovaries preservation [6][7][8]15]. Several studies indicated that the imbalance between ROS production and antioxidant activities could cause oxidative stress [7,23].…”

“…Several studies indicated that the imbalance between ROS production and antioxidant activities could cause oxidative stress [7,23]. In cow, oocyte meiotic competence could be maintained when ovaries were stored in saline supplemented with epigallocatechingallate compared to glutathione [6]. Experimental data from the feline model showed that oocytes from ovaries preserved in PBS supplemented with SOD reduced the percentage of apoptosis in COCs compared to control group [7].…”

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“…One of the largest outstanding problems associated with this technique is our current lack of knowledge concerning the effect of sperm capacitation and motility upon the subsequent development of injected oocytes. We have previously demonstrated that the methyl-β-cyclodextrin induced capacitation of sperm promoted fertilization in vitro [1]. We showed that single activated sperm caught could be caught us- …”