Antioxidantactivities <i>In Vitro</i> And Hepatoprotective Effects Of <i>Nelumbo Nucifera</i> Leaves <i>In Vivo</i>

Abstract

Background: Herbal medicines played a major role in the treatment of hepatic disorders, and a number of medicinal plants and their compounds were widely used for the treatment of these disorders, and oxidant stress injury was one of the mechanism of liver injury. Materials and Methods:Antioxidant activity of Nelumbo nucifera leaves (NU) extracts was assayed by the methods of scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2´-azino-bis (3-ethylbenzo-thiazoline-6-sulfonicacid) (ABTS) radical and ferric reducing antioxidant power (FRAP) in vitro. By intraperitoneal injection carbon tetrachloride (CCl 4 ) to establish acute liver injury model in mice, the levels of Glutamic-pyruvic transaminase (GPT), glutamic-oxaloacetic transaminase (GOT), superoxide dismutase (SOD) and the content of and maleicdialdehyde (MDA) were detected to evaluate hepatoprotective effect of NU using corresponding test kit.Results: EtOAC (NUEA) and n-BuOH extracts (NUBU) of N. nucifera leaves had good scavenging DPPH and ABTS radical activity and ferric reducing antioxidant power in vitro. DPPH radical scavenging activity and ferric reducing antioxidant power of NUEA (IC 50 = 6.68±0.29 µg/mL, RACT 50 =1749.82±67.03 µmol/g) and NUBU (IC 50 = 4.61±0.01 µg/mL, RACT 50 =1995.27±135.71 µmol/g ) were higher than that of BHT (IC 50 =8.76±0.20 µg/mL, RACT 50 =1581.68±97.41 µmol/g) and Dangfeiliganning (IC 50 =28.06±0.17 µg/mL, RACT 50 =1028.55±3.28 µmol/g).ABTS radical scavenging activity of NUEA (IC 50 = 5.32±0.12 µg/mL) and NUBU (IC 50 = 8.16±0.27 µg/mL) were higher than that of Dangfeiliganning (IC 50 = 9.76±0.16 µg/mL). Thus, hepatoprotective effect of NUEA and NUBU was evaluated on CCl 4 -induced acute liver injury mice. The results showed that the levels of GOT and GPT in each treatment group significantly decreased (p<0.001 and p<0.01, p<0.05, respectively) except for the group of NUEA (130.8 mg/kg) (p>0.05). The contents of malondialdehyde (MDA) in liver in groups of NUEA (523 mg/kg), NUBU (840.5 and 420.5 mg/kg, repectively) had significant decrease (p<0.001 and p<0.05, respectively), and the level of SOD in liver for each treatment group could significantly decrease (p<0.001, p<0.05, respectively).Conclusion: NUEA and NUBU had significantly hepatoprotective effect for Calcium tetrachloride CCl 4 -induced liver injury, which might be attributable to its antioxidant activity.

Protective effects of silymarin on triptolide-induced acute hepatotoxicity in rats

“…It has been reported that silymarin functions as an antioxidant by scavenging oxygen free radicals and decreasing lipid peroxidation to accelerate hepatocyte regeneration with minimal adverse actions ( 35 – 38 ). The results of the present study were consistent with previous studies ( 39 – 41 ). The activities of antioxidant enzymes, including SOD, CAT, GST and GSH-Px, were decreased, while MDA production increased in the liver of rats in the TP group.…”

Improvement Effect of Lotus Leaf Flavonoids on Carbon Tetrachloride-Induced Liver Injury in Mice

“…Lotus and its leaves have certain biological activities, in particular, some studies showed that they had good antioxidant activity, and their antioxidant activity also played a protective role in cells, especially on liver cells and liver [41][42][43][44]. In addition, the lotus seed was also rich in alkaloids, which could inhibit liver injury [1].…”

Hepatoprotective Effect of Silymarin (Silybum marianum) on Hepatotoxicity Induced by Acetaminophen inSpontaneously Hypertensive Rats

“…Various studies indicate that Silymarin exhibits strong antioxidant activity [ 36 ] and shows protective effects against hepatic toxicity induced by a wide variety of agents by inhibiting lipid peroxidation [ 30 , 37 ]. Higher total phenolic content has been known to contribute to the antioxidant activity of extracts [ 38 ], while antioxidant activity has also been linked to the hepatoprotective effect of some extracts [ 38 , 39 ]. These findings corroborate with our results on the ability of SLM to exert a hepatoprotective activity.…”