Antioxidants as a defence against reactive oxygen species

Marwicka, Justyna; Zięba, Anna

doi:10.52336/acm.2021.10.6.02

Cited by 7 publications

(4 citation statements)

References 3 publications

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“…Cows are particularly vulnerable to disturbance of homeostasis and one of the reasons is an intensification of milk yield in recent years [ 8 ]. Oxidative stress plays a crucial role in the occurrence of many diseases, reproductive disorders, and reduced milk yield [ 9 , 10 ]. Organisms have antioxidant systems that consist of antioxidant enzymes such as catalase, glutathione peroxidase, and superoxide dismutase, as well as non-enzymatic antioxidants: retinol, tocopherol, ascorbic acid, glutathione, α,α-diphenyl-β-picrylhydrazyl (DPPH), and others [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Dried Apple Pomace (DAP) as a Feed Additive on Antioxidant System in the Rumen Fluid

Bartel

Koszarska

Wysocki

et al. 2022

IJMS

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The study aimed to evaluate the effect of dried apple pomace (DAP) as a feed additive on the enzymatic activity and non-enzymatic compounds belonging to the antioxidant system in cattle rumen fluid. The experiment included 4 Polish Holstein–Friesian cannulated dairy cows and lasted 52 days. The control group was fed with the standard diet, while in the experimental group, 6% of the feedstuff was replaced by dried apple pomace. After the feeding period, ruminal fluid was collected. The spectrophotometric technique for the activity of lysosomal enzymes, the content of vitamin C, polyphenols, and the potential to scavenge the free DPPH radical was used. The enzyme immunoassay tests (ELISA) were used to establish the activity of antioxidants enzymes and MDA. Among the rumen aminopeptidases, a significant reduction (p < 0.01) from 164.00 to 142.00 was observed for leucyl-aminopeptidase. The activity of glycosidases was decreased for HEX (from 231.00 to 194.00) and β-Glu (from 1294.00 to 1136.00), while a significant statistically increase was noticed for BGRD (from 31.10 to 42.40), α-Glu (from 245.00 to 327.00), and MAN (from 29.70 to 36.70). Furthermore, the activity of catalase and GSH (p < 0.01) was inhibited. In turn, the level of vitamin C (from 22.90 to 24.10) and MDA (from 0.36 to 0.45) was statistically higher (p < 0.01). The most positive correlations were observed between AlaAP and LeuAP (r = 0.897) in the aminopeptidases group and between β-Gal and MAN (r = 0.880) in the glycosidases group. Furthermore, one of the most significant correlations were perceived between SOD and AlaAP (r = 0.505) and AcP (r = 0.450). The most negative correlation was noticed between α-Gal and DPPH (r = −0.533) based on these observations. Apple pomace as a feed additive has an influence on lysosomal degradation processes and modifies oxidation–reduction potential in the rumen fluid. Polyphenols and other low-weight antioxidant compounds are sufficient to maintain redox balance in the rumen.

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Section: Introductionmentioning

confidence: 99%

Effect of Dried Apple Pomace (DAP) as a Feed Additive on Antioxidant System in the Rumen Fluid

Bartel

Koszarska

Wysocki

et al. 2022

IJMS

View full text Add to dashboard Cite

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“…Furthermore, according to a study by Tan et al, gallic acid inhibited the expression of the main adipogenesis genes SREBP-1c and PPAR-γ by 37.2% and 51.1%, respectively, thus confirming its inhibition of adipogenesis effect through the inhibition of preadipocyte differentiation [26]. Catalase and peroxidase decompose the active oxygen generated in the body into water and oxygen molecules, thereby regulating homeostasis [27]. However, when an excessive production of active oxygen, lipid and protein denaturation, adipocyte metabolism disorders, and lowering of the body's metabolic function are present, a homeostasis imbalance occurs, which ultimately leads to obesity.…”

Section: Quantification Of Major Substance Using Hplcmentioning

confidence: 84%

Inhibition Effect of Adipogenesis and Lipogenesis via Activation of AMPK in Preadipocytes Treated with Canavalia gladiata Extract

Hong

Park

Kim

et al. 2023

IJMS

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The aim of this study was to investigate the effect of Canavalia gladiata extract (CGE) on the regulation of AMP-activated protein kinase (AMPK) in 3T3-L1 preadipocytes and evaluate the adipogenesis and lipogenesis mechanisms. In 3T3-L1 preadipocytes, lipid accumulation and differentiation were suppressed by 1.1, 1.3, and 1.4 times under the CGE treatment at 0.25, 0.5, and 1.0 mg/mL, respectively. The expression of the main genes involved in the inhibition of adipogenesis was evaluated at the mRNA level via a transcription-polymerase chain reaction. The extract at 1.0 mg/mL increased the mRNA expressions of AMPK and carnitine palmitoyl transferase-1 (CPT-1) by 1.9 and 1.2 times, respectively, while it decreased the expression of sterol regulatory element binding proteins-1c (SREBP-1c), peroxisome proliferator activated receptor-γ (PPAR-γ), CCAAT enhancer binding protein-α (C/EBP-α), and fatty acid synthase (FAS) by 1.1, 1.2, 1.8, and 1.5 times, respectively, indicating inhibition of the adipogenesis and lipogenesis potential of CGE. Gallic acid (4.02 mg/g) was identified as the main component of the CGE via LC-MS/MS and HPLC analysis. The results of this study suggested that CGE can be utilized as an anti-obesity food additive or medication by activating the AMPK-induced regulation and suppressing adipogenesis transcription factors.

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“…SOD2 is predominantly found in mitochondria. SOD3 is found in blood serum, tissues, and body fluids (including synovial fluid and cerebrospinal fluid) [139]. A study showed that SOD activity was elevated in rats with acetic acid-induced UC compared to the control group.…”

Section: Superoxide Dismutasementioning

confidence: 99%

Antioxidants as Protection against Reactive Oxidative Stress in Inflammatory Bowel Disease

2023

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Inflammatory bowel disease (IBD) belongs to a group of chronic diseases characterised by periods of exacerbation and remission. Despite many studies and observations, its aetiopathogenesis is still not fully understood. The interactions of genetic, immunological, microbiological, and environmental factors can induce disease development and progression, but there is still a lack of information on these mechanisms. One of the components that can increase the risk of occurrence of IBD, as well as disease progression, is oxidative stress. Oxidative stress occurs when there is an imbalance between reactive oxygen species (ROS) and antioxidants. The endogenous and exogenous components that make up the body’s antioxidant defence can significantly affect IBD prophylaxis and reduce the risk of exacerbation by neutralising and removing ROS, as well as influencing the inflammatory state.

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Antioxidants as a defence against reactive oxygen species

Cited by 7 publications

References 3 publications

Effect of Dried Apple Pomace (DAP) as a Feed Additive on Antioxidant System in the Rumen Fluid

Effect of Dried Apple Pomace (DAP) as a Feed Additive on Antioxidant System in the Rumen Fluid

Inhibition Effect of Adipogenesis and Lipogenesis via Activation of AMPK in Preadipocytes Treated with Canavalia gladiata Extract

Antioxidants as Protection against Reactive Oxidative Stress in Inflammatory Bowel Disease

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