Antioxidants attenuate oxidative damage in rat skeletal muscle during mild ischaemia

Abstract

We have previously shown oxidative stress and oedema, caused by both xanthine oxidasederived oxidants and infiltrating neutrophils, within skeletal muscle after contractile-induced claudication. The purpose of this study was to determine whether supplementation with antioxidant vitamins attenuates the oxidative stress, neutrophil infiltration and oedema associated with an acute bout of contractile-induced claudication. Rats received vehicle, vitamin C, vitamin E or vitamin C + E for 5 days prior to contractile-induced claudication. Force production was significantly reduced in the claudicant limbs of all groups compared with the control (sham) limb of control animals. Contractile-induced claudication caused a significant increase in protein oxidation, lipid peroxidation, neutrophil infiltration and oedema compared with sham muscles. Supplementation with vitamin C, E or C + E prevented the increases in each of these, and there were no differences between groups. These findings suggest that, in an animal model of exercise-induced claudication, neutrophil chemotaxis is caused by oxidizing species and that antioxidant supplementation can prevent oxidative damage, neutrophil infiltration and oedema following an acute bout of contractile-induced claudication.

Dietary implications on mechanisms of sarcopenia: roles of protein, amino acids and antioxidants

“…The antioxidant defense system can be augmented with appropriate dietary levels of antioxidants and/or supplementation. A number of studies have been conducted in an attempt to improve antioxidant systems via exogenous administration of antioxidant supplementation [119][120][121][122][123]. Rebrin et al [124] investigated the effects of supplementation with a combination of antioxidants on oxidative stress in skeletal muscle tissue homogenates and mitochondria in senescence-accelerated mice.…”

Oxytocin in The Prevention of Injury Due to Testicular Torsion/Detorsion in Rats

“…In this context, for preventing harmful effects of oxidative stress, many antioxidant agents, such as vitamin E, melatonin, retinol, β-carotene, omega-3, resveratrol, allopurinol, melatonin, Nacetylcysteine, zinc aspartate, and caffeic acid phenethyl ester, vitamin C, coenzyme Q10, and acetylcysteine have been used with different success rates. [46][47][48][49][50] Although numerous experimental animal studies have confirmed the efficacy of antioxidants in reducing short-term damaging effects of testicular torsion, different antioxidant agents have been investigated to reduce the short-and long-term reperfusion damage on the testes. Anti-apoptotic, anti-inflammatory, and antioxidant properties of oxytocin have been reported.…”

IκBα degradation is necessary for skeletal muscle atrophy associated with contractile claudication

“…For these reasons, the model used here has previously been used by our group, and others, to mimic exercise claudication (17,24,25,27). We have previously shown, using this model, that force production from the triceps surae muscle group decreases by ϳ10% in control limbs and ϳ50% in ligated limbs after 15 min of stimulation (24), and by ϳ15% and 70% in control and ligated limbs, respectively, after 30 mins of stimulation (24,25,27). The small decrease in control limbs demonstrates that the stimulation protocol is not intense.…”

“…Two days following the ligation or sham surgery, in vivo muscle contractions began using a stimulation protocol previously optimized to cause a significant decrease in force production from limbs in which the femoral artery is ligated, but not in control limbs (24,25,27). The decrease in force production indicates insufficient oxygen delivery to the muscles.…”