Antioxidants in Animal Tissue.

Glavind, J.; Jørgensen, Ole A; Røst, Erling; Steffensen, Arne; Munch‐Petersen, Jon

doi:10.3891/acta.chem.scand.17-1635

Cited by 99 publications

(41 citation statements)

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“…151.003) in the mass spectrum of 3 lent further support to this identification (Figure 3). The radical scavenging properties of compounds 1 (Table 2) and 2 (Table 3) were determined with the DPPH (diphenylpicrylhydrazyl) assay (Glavind, 1963;Lyckander & Malterud, 1996). Gallic acid has previously been reported to be a potent radical scavenger (Masaki et al, 1994) and the calculated IC 50 for this compound was 27.7 mM.…”

Section: Resultsmentioning

confidence: 99%

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Novel GHB-derived natural products from European mistletoe (Viscum album)

Amer

Juvik

Francis

et al. 2013

Pharmaceutical Biology

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Context: The European white-berry mistletoe [Viscum album L. (Loranthaceae)] is among the oldest known medicinal plants. At present the most important application of mistletoe extracts is in the treatment of cancer. However, natural products specific to mistletoe have rarely been encountered in the current literature. Objective: To discover novel natural products specific to European mistletoe. Materials and methods: European mistletoe was extracted with methanol, purified to partition against diethyl ether and further purified with XAD-7 column chromatography. Pure compounds were separated by Sephadex column chromatography and preparative HPLC. The structures of the novel compounds were established using a combination of several 2D NMR spectroscopic techniques and mass spectrometry. Results: A new type of natural product derived from the methyl ester of g-hydroxybutyric acid (GHB) coupled to hydroxybenzoic acids, namely 3-(3 0 -carbomethoxypropyl) gallic acid and 3-(3 0 -carbomethoxypropyl)-7!3 00 -protocatechoyl galloate were characterized from European white-berry mistletoe. Condensation of the 3-hydroxyl of gallic acid with the 4-hydroxyl of GHB significantly reduced the radical scavenging properties of the former compound. Discussion and conclusion: The characterized compounds define a novel group of natural products that may be of particular interest because it appears that the two new compounds are not closely related to any known natural product. Keywords2D NMR, 3-(3 0 -carbomethoxypropyl)gallic acid, 3-(3 0 -carbomethoxypropyl)-7!3 00 -protocatechoyl galloate, g -hydroxybutyric acidHistory

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Section: Resultsmentioning

confidence: 99%

“…These experiments were performed in accordance with the procedure described by Glavind (1963) and Lyckander and Malterud (1996), with some modifications outlined below.…”

Section: Dpph Scavenging Activitymentioning

confidence: 99%

Novel GHB-derived natural products from European mistletoe (Viscum album)

Amer

Juvik

Francis

et al. 2013

Pharmaceutical Biology

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“…The levels of water-soluble and fat-soluble antioxidants in the liver were determined by using a stable free radical, a,a,-diphenyl-(9 picrylhydrazyl (DPPH), according to the procedure of Glavind (11). The extent of lipid peroxidation induced by the addition of CC14 into the microsomal preparation was estimated by measuring malonic dialdehyde formed in the reaction medium as follows: Liver microsomes (2 mg protein) were preincubated at 37°C for 2 min in 0.2 M potassium phosphate buffer (pH 7.5) (12) with 400 i M NADPH in a final volume of 2 ml and further incubated in a tightly capped tube after adding 2 ,umoles CC14.…”

Section: Methodsmentioning

confidence: 99%

Therapeutic effect of taurine administration on carbon tetrachloride-induced hepatic injury.

Nakashima¹,

TANIKO²,

Kuriyama³

1982

Jpn.J.Pharmacol

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Abstract-To determine the therapeutic efficacy of taurine administration on liver injury, the effect of taurine on hepatic lipid peroxide level following carbon tetrachloride (CCI4) administration was investigated. To CCI4 intoxicated rats (CCI4, 2 ml/kg, i.p.), 3 ml of a 10% aqueous solution of taurine was administered after CCI4 administration (at 12, 16, and 20 hr after), and rats were sacrificed at 24 hr after CCI4 administration in order to estimate the level of lipid peroxides in the liver. The decrease of hepatic lipid peroxidation induced by CCI4 was noticed in the taurine-treated rats. Taurine had no effect on mixed function oxidase activity in hepatic micro somes as well as no effect on hepatic antioxidant content in the CC14 intoxicated rats. Hepatic taurine content decreased significantly 12 and 24 hr after CCI4 administration, whereas oral administration of taurine to CCI4-intoxicated rats was able to protect these rats from hepatic taurine depletion.These results suggest that hepatic taurine may play a critical role on the protection of hepatocytes against hepatotoxins such as CCI4 and the administration of taurine may be useful in the treatment of hepatotoxin induced liver injury.

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“…Determination of antioxidants: Water soluble antioxidant was measured by the use of the stable free radical 1,1 -dipheny!-2 picrylhydrazyl (DPPH) in the supernatant fraction prepared by 5% trichloroacetic acid (TCA) precipitation of tissue homogenates (12,13). The amount is expressed in terms of n equiv.…”

Section: Methodsmentioning

confidence: 99%

Alteration of Lipid Peroxide and Endogenous Antioxidant Contents in Retina of Streptozotocin-lnduced Diabetic Rats: Effect of Vitamin A Administration

Nishimura

Kuriyama

1985

Japanese Journal of Pharmacology

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Abstract-Possible involvement of lipid peroxide (LPO) in the occurrence of diabetic retinal lesion was investigated using streptozotocin-induced diabetic rats. Young male Wistar rats weighing 100-150 g were made diabetic by daily intraperitoneal injection of 30 mg/kg streptozotocin (STZ) for 5 days. Five weeks after the termi nation of STZ-treatment, when animals maintained typical hyperglycemia, the tissue level of LPO, estimated by the thiobarbituric acid method in the presence of 0.5 mM EDTA, was found to be augmented in the kidney. At 7 to 9 weeks after the STZ-treatment, the content of LPO in the retina also exhibited a significant increase, while those in the serum, brain and peripheral nerves showed no alteration. This increment of LPO in the kidney and retina was accompanied by the concomitant reduction of fat-soluble antioxidants determined by the ferric chloride-bipyridyl reaction, and insulin treatment (10 u/rat/day, s.c.) completely eliminated the increased formation of LPO in these organs. When diabetic rats were treated with retinol acetate, which had an inhibitory effect on LPO formation in retinal homogenate, the increase in LPO content was found to be significantly suppressed, especially in the retina. These results suggest that the STZ-induced diabetic state may elicit an increased formation of LPO in the retina and kidney, both of which are known to be main organs having typical diabetic lesions.

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Antioxidants in Animal Tissue.

Cited by 99 publications

References 0 publications

Novel GHB-derived natural products from European mistletoe (Viscum album)

Novel GHB-derived natural products from European mistletoe (Viscum album)

Therapeutic effect of taurine administration on carbon tetrachloride-induced hepatic injury.

Alteration of Lipid Peroxide and Endogenous Antioxidant Contents in Retina of Streptozotocin-lnduced Diabetic Rats: Effect of Vitamin A Administration

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