Antioxidants resveratrol and SkQ1 attenuate praziquantel adverse effects on the liver in Opisthorchis felineus infected hamsters

Global changes in gene expression related to Opisthorchis felineus liver fluke infection reveal temporal heterogeneity of a mammalian host response

“…Moreover, here, metabolic genes were significantly downregulated at 3 months p.i., indicating a general impairment of liver metabolism. These data overall are well consistent with our previously published results on hepatocyte changes in the form of fatty degeneration and excessive accumulation of lipids in the liver ( Zaparina et al, 2021 ). The excessive accumulation of neutral lipids in the liver may be related to pathological processes, including those taking place in response to toxic compounds ( Campos et al, 2020 ; Chaidee et al, 2018 ).…”

“…Opisthorchiidae species have strong proinflammatory properties ( Maeng et al, 2016 ; Pak et al, 2016 ; Pakharukova et al, 2019a , Pakharukova et al, 2019b ). During chronic inflammation, inflammatory cells are recruited to injury sites, thereby increasing a release and accumulation of free radicals, resulting in the formation of lipid peroxidation byproducts ( Pakharukova et al, 2019a ; Zaparina et al, 2021 ) as well as oxidative DNA lesions like 8-OHdG ( Maeng et al, 2016 ; Pakharukova et al, 2019a ). It was previously shown that TNF-α and CD163 protein levels increase in a strongly time-dependent manner in OF-infected golden hamsters ( Pakharukova et al, 2019a ).…”

“…For an immunohistochemical assay, the livers were immediately fixed in 4% paraformaldehyde in phosphate-buffered saline. The fixed livers were sliced at 7 or 10 μm thickness ( n = 4 to 5; 4–6 serial tissue sections per animal) on a Microm HM-505 N cryostat ( Pakharukova et al, 2019b ; Zaparina et al, 2021 ).…”

“…Four-μm-thick slices were prepared by means of a microtome. The tissue slices were stained with hematoxylin and eosin by the standard methods and were examined under a light microscope (Axioskop 2 Plus; Zeiss, Germany) ( Pakharukova et al, 2019b ; Zaparina et al, 2021 ).…”

Similarities and differences among the Opisthorchiidae liver flukes: insights from Opisthorchis felineus

“…During chronic inflammation, inflammatory cells are recruited to injury sites, thereby enhancing the release and accumulation of free radicals, resulting in the formation of lipid peroxidation byproducts, which can induce the formation of oxidative DNA lesions, e.g. 8-hydroxy-2′-deoxyguanosine (Maeng et al ., 2016; Pakharukova et al ., 2019 b ; Zaparina et al ., 2021).…”