Antioxidants screening in Limonium aureum by optimized on-line HPLC–DPPH assay

Dong, Geng; Chi, Xiaofeng; Qian, Dong; Hu, Fangdong

doi:10.1016/j.indcrop.2015.01.063

Cited by 44 publications

(29 citation statements)

References 31 publications

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“…It was deduced that the content of antioxidants might be lower after they reacted with DPPH. Consequently, the effective antioxidants could be selected by the degree of peak area reduction [35]. The peak areas of fifteen compounds in 75% EEFs are shown in Table 2.…”

Section: Screening Antioxidants Of Alfalfamentioning

confidence: 99%

Investigation of Chemical Composition, Antioxidant Activity, and the Effects of Alfalfa Flavonoids on Growth Performance

Chen

Liu

et al. 2020

Oxidative Medicine and Cellular Longevity

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The flavonoids were extracted from alfalfa using ethanol assisted with ultrasonic extraction and purified by D101 macroporous resin column chromatography. The chemical composition and content of ethanol elution fractions (EEFs) were assessed by ultrahigh-performance liquid chromatography and hybrid quadrupole time of flight mass spectrometry (UHPLC-Q-TOF-MS) and aluminum nitrate-sodium nitrite-sodium hydroxide colorimetric method. The in vitro antioxidant activity of two EEFs was conducted by scavenging DPPH free radical, and the main antioxidants of 75% EEFs were screened using DPPH-UHPLC. Moreover, the in vivo antioxidant activity of 75% EEFs and the growth performance of broilers were studied. The results showed that the content of 30% and 75% EEFs was 26.20% and 62.57%. Fifteen compounds were identified from 75% EEFs, and five of them were reported in alfalfa for the first time. The scavenging activity of 75% and 30% EEFs (200 μg/mL) against DPPH was 95.51% and 78.85%. The peak area of 5,3′,4′-trihydroxyflavone and hyperoside was decreased by 82.69% and 76.04%, which exhibited strong scavenging capacities. The total antioxidant capacity (T-AOC), superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX) level of three treated groups against the normal control group (NC) fed with basal diet significantly increased by 3.89-24.49%, 0.53-7.39%, and 0.79-11.79%, respectively. While the malondialdehyde (MDA) decreased by 0.47-18.27%. Compared with the NC, the feed to gain ratio (F : G) of three treated groups was lowered by 2.98-16.53% and survival rate of broilers significantly increased. Consequently, 75% EEFs extracted from alfalfa exhibited powerful antioxidant activities and might be a potential feed additive to poultry and livestock.

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Section: Screening Antioxidants Of Alfalfamentioning

confidence: 99%

Investigation of Chemical Composition, Antioxidant Activity, and the Effects of Alfalfa Flavonoids on Growth Performance

Chen

Liu

et al. 2020

Oxidative Medicine and Cellular Longevity

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“…ere are numerous local endemic species and, due to their isolation, many of them are threatened and protected species. Some species are cultivated as ornamental plants, whereas others have important medicinal properties [5,6]. Research on species of this genus has revealed important bioactivity concerning the free radical scavenging [7,8], antioxidant [5,[9][10][11], anti-inflammatory [10], antibacterial [12], antimicrobial [9], and antiviral [13] properties.…”

Section: Introductionmentioning

confidence: 99%

“…Some species are cultivated as ornamental plants, whereas others have important medicinal properties [5,6]. Research on species of this genus has revealed important bioactivity concerning the free radical scavenging [7,8], antioxidant [5,[9][10][11], anti-inflammatory [10], antibacterial [12], antimicrobial [9], and antiviral [13] properties. e main phenolics identified as responsible for these activities were gallic acid, epigallocatechin gallate, and myricetin and isorhamnetin flavonoids [7,8,10,11,14].…”

Section: Introductionmentioning

confidence: 99%

Phenolic Profile, Antioxidant Activity, and Enzyme Inhibitory Properties ofLimonium delicatulum(Girard) Kuntze andLimonium quesadenseErben

Ruiz-Riaguas

Zengin

Sinan

et al. 2020

Journal of Chemistry

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In this work, we report the phytochemical composition and bioactive potential of methanolic and aqueous extracts of leaves from Limonium delicatulum (Girard) Kuntze and Limonium quesadense Erben. The characterization and quantitation of individual phytochemicals were performed with liquid chromatography with diode array and electrospray-tandem mass spectrometry detection. Myricetin glycosides were abundant in L. delicatulum, whereas L. quesadense was rich in gallo(epi)catechin-O-gallate. Total phenolics, flavonols, and flavonoids were assayed with conventional methods. Antioxidant and radical scavenging assays (phosphomolybdenum, DPPH, ABTS, CUPRAC, FRAP, and metal chelating activity), as well as enzyme inhibitory assays (acetylcholinesterase, butyrylcholinesterase, tyrosinase, amylase, glucosidase, and lipase), were performed to evaluate the potential bioactivity. The methanolic extracts of both species presented higher phenolic content and bioactivity than the aqueous extracts. Overall, L. quesadense extracts exhibited the most potent activity for most assays, representing a potential source of bioactive compounds for the pharmaceutical and food industries.

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“…L. aureum attracts many researchers' attention for its medicinal, ornamental, and ecological values (Ye and Huang 2006). Previous studies mainly focused on the morphological and physiological aspects of L. aureum (Li et al 2007;Geng et al 2015;Liu et al 2016). The investigation on the molecular data of this species will contribute to the better study and utilization.…”

Section: Limonium Aureum; Chloroplast Genome; Phylogenetic Analysismentioning

confidence: 99%

Complete plastome sequence of Limonium aureum, a medicinal and ornamental species in China

Zhang

Liu

2019

Mitochondrial DNA Part B

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Limonium aureum is a perennial herb of Plumbaginaceae, and draw the attention of researchers by its medicinal and ecological value. In this study, we first report the complete chloroplast genome of L. aureum with paired-end sequencing method. The results showed that the complete chloroplast genome of L. aureum is 154,661 bp in length with a typical quadripartite structure, including a large single-copy region (LSC, 84,545 bp), a single-copy region (SSC, 12, 980 bp), and a pair of inverted repeats (IRs, 28,568 bp). There are 113 annotated genes, consisting of 79 unique protein-coding genes, 4 unique ribosomal RNA genes, and 30 transfer RNA genes. Moreover, we constructed a phylogenetic tree with L. aureum and other 34 species based on their complete chloroplast genomes. And the results of the phylogenetic topologies displayed that Plumbago auriculata was closely related to L. aureum. Our results will contribute to the better study and make use of the species.

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Antioxidants screening in Limonium aureum by optimized on-line HPLC–DPPH assay

Cited by 44 publications

References 31 publications

Investigation of Chemical Composition, Antioxidant Activity, and the Effects of Alfalfa Flavonoids on Growth Performance

Investigation of Chemical Composition, Antioxidant Activity, and the Effects of Alfalfa Flavonoids on Growth Performance

Phenolic Profile, Antioxidant Activity, and Enzyme Inhibitory Properties ofLimonium delicatulum(Girard) Kuntze andLimonium quesadenseErben

Complete plastome sequence of Limonium aureum, a medicinal and ornamental species in China

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