Antioxidation Effect of Leg Bone Extracts and Enzyme Hydrolysates from Jeju Crossbred Horses (Jeju Native Horse×Thoroughbred)

Abstract

This study was conducted to evaluate the antioxidation activity of Jeju crossbred horse (Jeju native horse × thoroughbred) leg bone extracts (HLBE) and its enzyme hydrolysates by determination of 1,1-diphenyl-2picryl-hydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzo thiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity, ferric reducing/antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC). HLBE was extracted with hot water for 24 hr and lyophilized. The lyophilized HLBE was hydrolyzed using multifect PR6L (MP), pepsin (PS), and a pepsin and pancreatin mixture (PSPC) for 4 hr at 60, 50, and 50°C, respectively. The hydrolysates were separated by a molecular weight of 3 kDa more or less. When the yield of HLBE was 100%, the yield of hydrolysates less than 3 kDa of MP, PS, and PSPC was 10.86, 3.26, and 8.00%, respectively. Enzyme hydrolysates with low molecular weight less than 3 kDa in MP and PSPC showed significantly higher DPPH, ABTS radical scavenging activity, and ORAC compared to HLBE and its hydrolysates with more than 3 kDa. However, the FRAP of the hydrolysates less than 3 kDa in PS was significantly higher than in MP. These results suggest that low molecular weight enzyme hydrolysates less than 3 kDa have more powerful antioxidation activity, especially when they are hydrolyzed by MP and PSPC rather than PS. Therefore, low molecular weight enzyme hydrolysates of HLBE hydrolyzed with MP and PSPC have significant potential as antioxidants in the food industry.Further in vivo studies are required to support the antioxidation activities of the hydrolysates in vitro.

Anti-oxidation and Anti-wrinkling Effects of Jeju Horse Leg Bone Hydrolysates

“…5 ). Similar results were reported by Kim et al . (2013) who found that the ORAC activity of pig skin gelatin hydrolysates increased with dose, and 1 mg/mL of pig skin gelatin hydrolysates had 141.39 μM TE/g of ORAC activity.…”

“…LMP from JHLB significantly reduced ABTS radicals in a dose dependent manner at concentrations ranging from 0.1 to 15 mg/mL. 15 and 20 mg/mL of LMP from JHLB also had ABTS radical scavenging effects that are equivalent to 100 μg/mL of vitamin C. Similarly, Kim et al . (2013) reported that Jeju horse hydrolysates (less than 3 kDa) generated by pepsin and pancreatin hydrolysis showed 0.48 mM TE of ABTS radical scavenging activity.…”

“…As shown, the DPPH radicals were significantly scavenged by LMP from JHLB in a dose dependent fashion ( p <0.05). In our previous study, low molecular weight (less than 3 kDa) peptides from Jeju horse showed higher DPPH radical scavenging activity than hydrolysates over 3 kDa ( Kim et al ., 2013 ). The ABTS radical scavenging activity of LMP from JHLB is shown in Fig.…”

“…In this study, the ORAC value of low molecular weight peptides from JHLB at a concentration of 1 mg/mL was 101.85 μM TE, which was lower than the ORAC value for pig skin gelatin hydrolysates. However, Kim et al . (2013) stated that the ORAC values of horse leg bone hydrolysates of less than 3 kDa produced with pepsin, or multifect, or pepsin+pancreatin were 0.12, 0.20, and 0.17 mM TE/g, respectively.…”

“…A study found that dietary supplementation with horse leg bone water extracts for 8 weeks significantly increases the uterine/body weight ratio and bone mineral density of female rats with postmenopausal osteoporosis ( Park et al ., 2010 ). In our previous study, a large antioxidant effect was observed in the low molecular weight peptide fraction of less than 3 kDa isolated from leg bone extracts via enzyme hydrolysis ( Kim et al ., 2013 ). However, there is still a lack of scientific information about the anti-oxidant and anti-wrinkle activities of enzyme hydrolysates from horse leg bone extracts.…”