Antioxidative Action of Ellagic Acid—A Kinetic DFT Study

Tošović, Jelena; Bren, Urban

doi:10.3390/antiox9070587

Cited by 51 publications

(43 citation statements)

References 48 publications

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“…In concert with these findings, in the present study, BCB showed strong scavenging activities against DPPH and superoxide anion radicals and inhibited ROS production in CHO-K1 cells treated with H 2 O 2 . Notably, ellagic acid and chlorogenic acid in BCB have strong antioxidant effects [59,60]. Although the present study did not demonstrate that the mechanism of action of BCB is directly related to antioxidant activity, evidence suggests that antioxidant activity is a contributory factor.…”

Section: Discussioncontrasting

confidence: 78%

Administration of a herbal formulation enhanced blastocyst implantation via IκB activation in mouse endometrium

et al. 2020

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Background BaelanChagsangBang (BCB), a herbal formulation consisting of eleven herbs, may be prescribed as a reproductive functional supplement to improve ovulation and implantation during the treatment of infertility and recurrent abortion in Korean Medicine. This study aimed to investigate the effects and action mechanisms of water-extracted BCB on endometrial receptivity and blastocyst implantation under normal conditions and in a mifepristone (RU486)-induced implantation failure murine model. Methods In vitro, the antioxidant potentials of BCB were evaluated using DPPH and superoxide anion radical scavenging assays and a DCFH-DA assay, and the cytotoxic and cytoprotective effects of BCB were confirmed using an MTT assay. In vivo, C57BL/6 female mice (n = 6 per group) orally received BCB (300 mg/kg/day), a dose similar to that used clinically, from 7 days before pregnancy until the end of the experiment. On day 4 of pregnancy, RU486 (4 mg/kg) was injected subcutaneously to induce implantation failure. The effect of BCB on embryo implantation was evaluated by implantation rate analysis, histological examination, and western blotting of uterus tissues. Results BCB water extract showed strong anti-oxidative and cytoprotective effects in vitro. In vivo administration of BCB water extract increased the number of newborn pups in BCB-treated mice versus sham-treated mice under normal conditions and improved the number of implantation sites in pregnant mice despite RU486 injection. BCB increased the protein levels of cyclooxygenase-2 and inducible nitric oxide synthase through IκB activation. Moreover, the expression levels of matrix metalloproteinases at uterus implantation sites were up-regulated in the BCB-treated group as compared with those in the RU486-treated group. Conclusion These results show BCB improved embryo implantation through IκB activation in our mouse model and suggest that BCB has therapeutic potential in the context of poor endometrial receptivity.

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Section: Discussioncontrasting

confidence: 78%

Administration of a herbal formulation enhanced blastocyst implantation via IκB activation in mouse endometrium

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In concert with these ndings, in the present study, BCB showed strong scavenging activities against DPPH and superoxide anion radicals and inhibited ROS production in CHO-K1 cells treated with H 2 O 2 . Notably, ellagic acid and chlorogenic acid in BCB have strong antioxidant effects [59,60]. Although the present study did not demonstrate that the mechanism of action of BCB is directly related to antioxidant activity, evidence suggests that antioxidant activity is a contributory factor.…”

Section: Discussioncontrasting

confidence: 78%

Administration of a herbal formulation enhanced blastocyst implantation via IκB activation in mouse endometrium

Jeon

Liu

Cai

et al. 2020

Preprint

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Background: BaelanChagsangBang (BCB), a herbal formulation consisting of eleven herbs, may be prescribed as a reproductive functional supplement to improve ovulation and implantation during the treatment of infertility and recurrent abortion in Korean Medicine. This study aimed to investigate the effects and action mechanisms of water-extracted BCB on endometrial receptivity and blastocyst implantation under normal conditions and in a mifepristone (RU486)-induced implantation failure murine model.Methods: In vitro, the antioxidant potentials of BCB were evaluated using DPPH and superoxide anion radical scavenging assays and a DCFH-DA assay, and the cytotoxic and cytoprotective effects of BCB were confirmed using an MTT assay. In vivo, C57BL/6 female mice (n = 6 per group) orally received BCB (300 mg/kg/day), a dose similar to that used clinically, from 7 days before pregnancy until the end of the experiment. On day 4 of pregnancy, RU486 (4 mg/kg) was injected subcutaneously to induce implantation failure. The effect of BCB on embryo implantation was evaluated by implantation rate analysis, histological examination, and western blotting of uterus tissues.Results: BCB water extract showed strong anti-oxidative and cytoprotective effects in vitro. In vivo administration of BCB water extract increased the number of newborn pups in BCB-treated mice versus sham-treated mice under normal conditions and improved the number of implantation sites in pregnant mice despite RU486 injection. BCB increased the protein levels of cyclooxygenase-2 and inducible nitric oxide synthase through IκB activation. Moreover, the expression levels of matrix metalloproteinases (MMPs) at uterus implantation sites were up-regulated in the BCB-treated group as compared with those in the RU486-treated group. Conclusion: These results show BCB improved embryo implantation through IκB activation in our mouse model and suggest that BCB has therapeutic potential in the context of poor endometrial receptivity.

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“…The kinetic studies of antioxidative action of ellagic acid (EA) under physiological conditions elucidated that the hydroxyl radical (•OH) with EA conforms to hydrogen atom transfer and radical adduct formation mechanisms, whereas the sequential proton loss electron transfer mechanism is responsible for the scavenging of the CCl 3 OO• radical, generating in the organism during the metabolism of CCl 4 . In addition, compared to trolox, EA was found to be more reactive toward •OH, but less reactive toward CCl 3 OO• in which their calculated rate constants are in very good agreement with the corresponding experimental values [ 2 ]. From another viewpoint of antioxidant mechanisms, the computational study for antioxidant mechanisms was carried out using total enthalpy values on the electronic effects of ortho-substituents in dendritic tri-phenolic antioxidants, comprising a common phenol moiety and two other phenol units with electron-donating or electron-withdrawing substituents.…”

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confidence: 77%