Antioxidative Activity and Antidiscoloration Efficacy of Ergothioneine in Mushroom (Flammulina velutipes) Extract Added to Beef and Fish Meats

Bao, Huynh Nguyen Duy; Ushio, Hideki; Ohshima, Toshiaki

doi:10.1021/jf8017063

Cited by 91 publications

(90 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The extract from edible mushroom, Flammulina velutipes, has been reported to be a promising source of natural antioxidants and melanosis-inhibiting agents (Jang, Sanada, Ushio, Tanaka, & Ohshima, 2002. It contains a significant amount of 2-thioll-histidine-betaine (ergothioneine, ESH) (Bao, Ushio, & Ohshima, 2008. Although thiol-containing compounds, such as ESH, are known to inhibit PO activity, no studies have assessed the effect of such compounds on the gene expression of proPO, a precursor of PO that plays an important role in melanosis formation in live crustaceans.…”

Section: Introductionmentioning

confidence: 99%

Biochemical intervention of ergothioneine-rich edible mushroom (Flammulina velutipes) extract inhibits melanosis in crab (Chionoecetes japonicus)

et al. 2011

Self Cite

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Biochemical intervention of ergothioneine-rich edible mushroom (Flammulina velutipes) extract inhibits melanosis in crab (Chionoecetes japonicus)

et al. 2011

Self Cite

View full text Add to dashboard Cite

“…DPPH scavenging rate of 65.85% and hydroxyl radical scavenging rate of 71.24% were obtained at 200 μg/mL of FvP-3. Previous studies reported that mushroom extracts had free radical scavenging and antioxidative activities not only in vitro but also in vivo (Bao et al, 2008;Cheung and Cheung, 2005;Jang et al, 2004;Puttaraju et al, 2006). Alcoholic and aqueous extracts of F. velutipes have been evaluated for antioxidant activity by lipoxygenase inhibition, DPPH assay and deoxyribose assays (Bao et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

In Vitro Antioxidant and Antitumor Activities of Polysaccharides Extracted from the Mycelia of Liquid-Cultured Flammulina velutipes

Zhao

Liu

et al. 2013

FSTR

View full text Add to dashboard Cite

Orthogonal experiment was used to optimize the extraction conditions of Flammulina velutipes mycelia polysaccharides (FvP). Four independent variables (ratio of water to raw material, initial pH value, extracting temperature, and extracting time) were taken into consideration. A yield of FvP of 2.19% was obtained under an optimized condition (ratio of water to material of 50:1, initial pH value of 6.0, extracting temperature of 85℃, and extracting time of 6 h). Subsequently, antioxidative properties of FvP-2 (crude polysaccharides) and FvP-3 (deproteinized polysaccharides) prepared from F. velutipes mycelia were evaluated by monitoring the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and hydroxyl radical scavenging activity, together with antitumor activity against the human hepatocellular carcinoma BEL-7402 cells. DPPH scavenging rate of 65.85% and hydroxyl radical scavenging rate of 71.24% were obtained at 200 μg/mL of FvP-3. The inhibition rate of BEL-7402 cells was up to 45% at 640 μg/mL of FvP-2. These results suggested that FvP possesses potent antioxidant and antitumor properties. The polysaccharide may be useful as a functional food additive and an antioxidant and antitumor agent. IntroductionMushrooms have been valued as edible and medicinal resources, and antitumor substances have been identified in many mushroom species. Polysaccharides, as one of the most important components isolated from mushrooms, have been correlated with multiple pharmacological activities, such as antioxidant, immunomodulatory, reducing blood lipid and antitumor activities. Flammulina velutipes, one of the most popular edible mushrooms, has being under a large-scale artificial cultivation and increasingly consumed in Japan and China owing to its high nutritional values and attractive taste (Smiderle et al., 2008). It has attracted considerable attention in the fields of its biological activities (Fu et al., 2002;Shi et al., 2012, Wasser, 2002. It was reported that the polysaccharides from the fruiting bodies of F. velutipes have been proved to be beneficial in immunomodulatory, antioxidant, antitumor and biological activity on hepatocytes (Leung et al., 1997;Ohkuma et al., 1983;Pang et al., 2007;. Several polysaccharides have been isolated from F. velutipes, and their pharmacological efficacies were investigated . For example, an antitumor polysaccharide with the structure of 1,3-glucan was isolated from cytoplasms of the fruiting bodies of F. velutipes (Ikekawa et al., 1982). An alkaline-soluble polysaccharide prepared from the cell wall of F. velutipes was reported to have immunomodulatory and antitumor activities against sarcoma SC180 (Leung et al., 1997). However, few studies on the bioactive of F. velutipes mycelia polysaccharides (FvP) were investigated. Furthermore, there are few investigations focusing on the effect of FvP on the proliferation of human hepatocellular cancer cells .Various methods are applied to increase the extraction efficiency of polysaccharides from mushrooms, including selection of proper sol...

show abstract

“…ERGO exerts its antioxidant activity at sub-mM to mM concentrations [34][35][36], whereas the hepatic ERGO concentration in control and DMN-treated mice was at most 100~200 µM (Fig. 3D).…”

Section: Discussionmentioning

confidence: 98%

Localization of Xenobiotic Transporter OCTN1/SLC22A4 in Hepatic Stellate Cells and Its Protective Role in Liver Fibrosis

Tang

Masuo

Sakai

et al. 2016

Journal of Pharmaceutical Sciences

View full text Add to dashboard Cite

3Xenobiotic transporters play key roles in disposition of a certain therapeutic agents although limited information is available on their roles other than pharmacokinetic issues. Here, suppressive effect of multispecific organic cation transporter OCTN1/SLC22A4 on liver fibrosis was proposed in liver injury models. After injection of hepatotoxins such as dimethylnitrosamine (DMN) or concanavalin A, hepatic fibrosis and oxidative stress, evaluated in terms of Sirius red and 4-hydroxy-2-nonenal staining, respectively, were more severe in liver of octn1/slc22a4 gene knockout (octn1 -/-) mice than that in wild-type mice. DMN treatment markedly increased α-smooth muscle actin

show abstract

Antioxidative Activity and Antidiscoloration Efficacy of Ergothioneine in Mushroom (Flammulina velutipes) Extract Added to Beef and Fish Meats

Cited by 91 publications

References 28 publications

Biochemical intervention of ergothioneine-rich edible mushroom (Flammulina velutipes) extract inhibits melanosis in crab (Chionoecetes japonicus)

Biochemical intervention of ergothioneine-rich edible mushroom (Flammulina velutipes) extract inhibits melanosis in crab (Chionoecetes japonicus)

In Vitro Antioxidant and Antitumor Activities of Polysaccharides Extracted from the Mycelia of Liquid-Cultured Flammulina velutipes

Localization of Xenobiotic Transporter OCTN1/SLC22A4 in Hepatic Stellate Cells and Its Protective Role in Liver Fibrosis

Contact Info

Product

Resources

About