Antioxidative Activity and Phytochemistry Profile of Hibiscus Sabdariffa Herb Extracts

Fitrotunnisa, Qotrunnada; Arsianti, Ade; Tejaputri, Nadzila Anindya; Qorina, Fona

doi:10.22159/ijap.2019.v11s6.33532

Cited by 11 publications

(7 citation statements)

References 8 publications

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“…This results further supports the basic antioxidant activities of S. nodiflora which has already been published [5]. Antioxidants protect the body against various reactive oxygen species [30], which otherwise are linked to the etiology of several diseases. The phytochemistry of S. nodiflora revealed the presence of flavonoids, tannins, saponins, alkaloids, cardiac glycosides, coumarins, triterpenes, sterols, anthraquinones and phenols.…”

Section: Discussionsupporting

confidence: 89%

Antioxidant and Anti-Proliferative Effects of an Ethyl Acetate Fraction of the Hydro-Ethanolic Extract of Synedrella Nodiflora (L) Gaertn

Amoateng

Bekoe

Amponsah

et al. 2020

Int J Pharm Pharm Sci

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Objective: Synedrella nodiflora is traditionally used in the treatment of several ailments. Pharmacologically, this plant has anticonvulsant, sedative, anti-nociceptive and anti-proliferative effects. This study further investigated S. nodiflora for its antioxidant and in vitro inhibition of cancerous cell lines. Methods: Phytochemical assays, and the DPPH radical scavenging method were employed in preliminary screening for antioxidant activities of the crude hydro-ethanolic extract (SNE) and resulting fractions. The potent ethyl acetate fraction (EAF), was further investigated for total phenol and flavonoid contents, reducing power, lipid peroxidation potential, and cytotoxic effects on human breast cancer (MCF-7), leukemic (Jurkat), and normal liver (Chang’s liver) cell lines. Results: The extract contained phenols, flavonoids, tannins, glycosides, sterols, terpenoids, and alkaloids. It scavenged for DPPH with an IC50 of 114 µg/ml, whereas that of EAF was 8.9 µg/ml. EAF prevented peroxidation of egg lecithin at an IC50 of 24.01±0.08 µg/ml. These IC50s are four and three times lower than the reference standards. EAF produced anti-proliferative effects against MCF-7, and Jurkat cell lines with IC50s of 205.2 and 170.9 µg/ml, respectively. EAF had a high IC50 of 252.2 µg/ml against Chang’s liver cells. At 0.1 mg/ml EAF had similar total flavonoid content to SNE, but a significantly higher total phenol content. Conclusion: The ethyl acetate fraction of S. nodiflora, exhibited the most potent antioxidant activity. It inhibited the proliferation of breast and leukemic cancer cell lines, whiles having weak cytotoxic effect on normal liver cells. These can be explored for further drug development.

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Section: Discussionsupporting

confidence: 89%

Antioxidant and Anti-Proliferative Effects of an Ethyl Acetate Fraction of the Hydro-Ethanolic Extract of Synedrella Nodiflora (L) Gaertn

Amoateng

Bekoe

Amponsah

et al. 2020

Int J Pharm Pharm Sci

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“…Anti-free radical test: DPPH Preparation of the 0.04 g/l DPPH solution The method described by Fitrotunnisa et al [18] was used. The DPPH reagent (10 mg) was dissolved in 25 ml of methanol.…”

Section: Evaluation Of Antioxidant Activitymentioning

confidence: 99%

Investigating the Antibacterial, Antioxidant, and Anti-Inflammatory Activities of Aqueous and Hydroethanolic Extracts of Ocimum Basilicum and Ocimum Gratissimum on Some Germs Responsible for Aerobic Vaginitis

Djova

FLORENTINE²,

ARLETTE³

et al. 2023

Int J Pharm Pharm Sci

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Objective: The present work evaluates the antibacterial, antioxidant, and anti-inflammatory activities of aqueous and hydroethanolic extracts of Ocimum basilicum and Ocimum gratissimum on germs responsible for aerobic vaginitis. Methods: University Teaching Hospital of Yaoundé and Central Hospital of Yaoundé provided the germ (Staphylococcus aureus) and isolates (Enterobacter cloacae and Providencia stuartii). The extracts were obtained by maceration of the plants in water and hydroethanolic system. Phytochemical screening was assessed using the standard method; sensitivity and inhibition tests were carried out on agar medium and microplates in liquid medium. The antioxidant activity of the extracts was evaluated using the 2,2-diphenyl-1-picrilhidrazil (DPPH), 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), ferric reducing antioxidant power assay (FRAP), and β-carotene assays; total polyphenols content was obtained by the Folin Ciocalteu assay; anti-inflammatory activity by denaturation of ovalbumin. Results: Flavonoids, catechin, and tannins were abundant in the hydroethanolic extracts (56.65±3.89 and 228.94±8.42 mg EAA/g, respectively). The extracts were bactericidal for S. aureus and bacteriostatic for P. stuartii and E. cloacae. The hydroethanolic extract of the leaves of O. gratissimum has the best anti-radical activity 23.08±3.12, while that of the leaves of O. basilicum had the best-reducing power (388.36±9.96). The anti-inflammatory activity was found to be significant in the leaf extracts studied with IC50 values of 0.358±0.013 mg/ml for O. basilicum to 0.269±0.008 mg/ml for O. gratissimum, thus justifying their anti-inflammatory properties. The equivalent contents of primary and secondary antioxidants of the extracts were also obtained. Conclusion: The hydroethanolic extracts of Ocimum basilicum and Ocimum gratissimum have antibacterial, antioxidant, and anti-inflammatory properties.

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“…Then stored at 4C. 10 mg/ml dimethyl sulfoxide (DMSO) was dissolved for further analysis modified [17].…”

Section: Plant Sample Collection and Preparation Of Extractsmentioning

confidence: 99%

Cytotoxic Activities of Cymbopogon citratus Extracts Three types of human cancer cell lines were tested: Lung Carcinoma (A549), Breast Cancer (MCF-7) and Hepatocellular Carcinoma (Hep G2)

Sayed¹,

issa

Mahmoud

et al. 2022

Journal of Modern Research

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Cymbopogon citratus, commonly known as Lemon grass is a small herbaceous plant of the Poaceae family. It is used as traditional medicine for the treatment of numerous diseases such as cancer, fever, sore throats, cough, laryngitis, bronchitis, body ache, headache, digestive problems, etc. This study deals with the cytotoxic activities of C. citratus against three different human cancer cells A549, HepG2, and MCF-7. The chemical fractionation of the plant material using ethanol, ethyl acetate, and hexane was prepared and analyzed for bioactivity. Ethyl acetate extract showed the highest anticancer activity against A549, HepG2, and MCF-7 human cancer cells with cell viability percentage (64.09±1.11, 41.22± 0.34, and 66.24± 1.50%, respectively) with IC 50 values (147, 65, and 155µg/ml, respectively). Results of this study show that the ethyl acetate fraction of C. citratus is a rich source of phenolics and other compounds that can play a significant role in preventing the progression of cancer diseases.

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Antioxidative Activity and Phytochemistry Profile of Hibiscus Sabdariffa Herb Extracts

Cited by 11 publications

References 8 publications

Antioxidant and Anti-Proliferative Effects of an Ethyl Acetate Fraction of the Hydro-Ethanolic Extract of Synedrella Nodiflora (L) Gaertn

Antioxidant and Anti-Proliferative Effects of an Ethyl Acetate Fraction of the Hydro-Ethanolic Extract of Synedrella Nodiflora (L) Gaertn

Investigating the Antibacterial, Antioxidant, and Anti-Inflammatory Activities of Aqueous and Hydroethanolic Extracts of Ocimum Basilicum and Ocimum Gratissimum on Some Germs Responsible for Aerobic Vaginitis

Cytotoxic Activities of Cymbopogon citratus Extracts Three types of human cancer cell lines were tested: Lung Carcinoma (A549), Breast Cancer (MCF-7) and Hepatocellular Carcinoma (Hep G2)

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