Antioxidative and cytoprotective response elicited by molybdenum nanoparticles in human cells

Akhtar, Mohd Javed; Ahamed, Maqusood; Alhadlaq, Hisham A.; Alshamsan, Aws; Khan, M.A. Majeed; Alrokayan, Salman A.

doi:10.1016/j.jcis.2015.07.034

Cited by 49 publications

(23 citation statements)

References 47 publications

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“…A recent study has reported Mo NPs to be nontoxic in MCF-7 and HT-1080 cell lines. Furthermore, Mo NPs seemed to significantly protect these cells from death induced by H 2 O 2 and ZnO NPs [17]. By contrast, a study by Siddiqui et al reported that Mo NPs induced cytotoxicity, oxidative stress, and genotoxicity in mouse skin fibroblast cells (L929) [16].…”

Section: Discussionmentioning

confidence: 87%

“…There is little information available on the toxic and nontoxic effects of Mo NPs. According to recent publications, Mo NPs could induce cytotoxicity, oxidative stress, and genotoxicity in mouse skin fibroblast cells and show cytoprotective effects in MCF-7 and HT-1080 cells [16,17]. The aim of this study was to investigate the toxicity potential of Mo NPs on hematological and biochemical parameters, sexual hormones, and also to carry out a histopathological examination of testis and liver tissues using male Sprague-Dawley rat as an animal model.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Molybdenum Nanoparticles on Blood Cells, Liver Enzymes, and Sexual Hormones in Male Rats

Asadi

Mohseni

Noshahr

et al. 2016

Biol Trace Elem Res

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Despite an increasing surge in application of nanoparticles in industries, there is a serious lack of information concerning their impact on human health and the environment. The present study investigated effects of molybdenum nanoparticles (Mo NPs) injected intraperitoneally into Sprague-Dawley rats at different doses of Mo NPs (5, 10, and 15 mg/kg BW per day) during a period of 28 days. Hematological and biochemical parameters as well as sexual hormones and histopathological examinations of the liver and testis were assessed and compared with control group. The results showed that the serum levels of testosterone decreased significantly in both groups of 10 and 15 mg (Mo NPs)/kg BW in comparison with the control group (p < 0.05). However, there were insignificant differences observed in luteinizing hormone (LH) levels and hematological parameters when compared with the control group (p > 0.05). The results of liver enzymes showed that serum levels of aspartate aminotransferase (AST) decreased significantly in both dosage groups of 5 and 10 mg/kg BW (Mo NPs) when compared with the control group (p < 0.05), and significant decrease obtained in lactate dehydrogenase (LDH) levels at dose of 5 mg/kg BW in comparison with the control group (p < 0.05). The histopathological examination of testis showed a decrease in number of Leydig cells. Also, the number of chronic inflammatory cells increased in portal triad and parenchyma in liver tissue of rats exposed to Mo NPs.

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Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Effect of Molybdenum Nanoparticles on Blood Cells, Liver Enzymes, and Sexual Hormones in Male Rats

Asadi

Mohseni

Noshahr

et al. 2016

Biol Trace Elem Res

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“…by showing protective effect in contact with peroxide compounds (H 2 O 2 ) and ZnO-NPS, which has been demonstrated for cell lines of human mammary gland adenocarcinoma (MCF-7) and fibrosarcoma (HT-1080). Mo NPs have been reported to significantly increase glutathione content in MCF-7 line (1.6-fold) and HT-1080 line (1.3-fold), which could be compared to the effect of antioxidant drug N-acetylcysteine (NAC) [25].…”

Section: Biochemical Blood Parameters Inmentioning

confidence: 99%

MORPHOLOGICAL AND BIOCHEMICAL PARAMETERS IN Wistar RATS INFLUENCED BY MOLYBDENUM AND ITS OXIDE NANOPARTICLES

Sizova¹,

Мирошников²,

Калашников³

2016

S-h. biol.

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A b s t r a c t Despite widespread use of nanoparticles in industry and medicine, there is very little information about how the newly developed nanomaterials interact with biological objects. Certain properties of the Mo-containing nanoparticles (NPs) suggest their possible toxic effect on warmblooded animals. In this paper we compared the effect of Mo NPs (at 1 and 25 mg/kg) and its oxide MoO3 NPs (at 1.2 and 29 mg/kg), when administrated parenterally, on metabolic parameters and the exchange of chemical elements in Wistar laboratory rats. There, we assessed the red and white blood cell counts, the hemoglobin level, the activity of catalase (CAT) and superoxide dismutase (SOD) (for oxidative status), the ALT, AST, LDH, GGT, creatine kinase activity, blood creatinine, bilirubin and urea concentrations (for metabolic status) at days 1, 7 and 14. A day after Mo NPs and MoO 3 NPs administration the number of blood leukocyte lowered by 11.3 % (Р < 0.05) and 58.5 % (Р < 0.01), respectively. Also, a decrease in monocyte number by 18.9 (Р < 0.05), 41.9 (Р < 0.01), 51.7 (Р < 0.05) and 83.3 % (Р < 0.001) as depending on NPs chemical composition and doses was characteristic, though on day 14 a significant difference to control (54.5 %, Р < 0.05) was found only for MoO 3 NPs at a dose of 29 mg/kg. The number of thrombocytes was the highest on day 14 for the maximum dosage of both NPs leading to hindered blood microcirculation. The experiments also showed an increase in serum aminotransferases, GGT and LDH activity. In sum, we observed manifestations of oxidative stress, anemia and capillary-trophic insufficiency in the animals administrated with high doses of molybdenum and Mo oxide NPs. These signs were progressing and the most apparent for molybdenum oxide NPs. Given the comparable doses used, the molybdenum nanoparticles exhibit lower toxicity as compared to its oxide.

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“…These clusters can be stimulated to generate the death of tumor cells [36] by inducing apoptosis through caspase activation [37], its antioxidant properties [38], or as a substitute for chemotherapy [39]. Molybdenum clusters have also shown photosensitizer properties, becoming promising components for photodynamic therapy, which requires agents that exert cytotoxic effects only under photoirradiation [40].…”

Section: Resultsmentioning

confidence: 99%

The [Mo6Cl14]2− Cluster is Biologically Secure and Has Anti-Rotavirus Activity In Vitro

et al. 2017

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The molybdenum cluster [Mo6Cl14]2− is a fluorescent component with potential for use in cell labelling and pharmacology. Biological safety and antiviral properties of the cluster are as yet unknown. Here, we show the effect of acute exposition of human cells and red blood cells to the molybdenum cluster and its interaction with proteins and antiviral activity in vitro. We measured cell viability of HepG2 and EA.hy926 cell lines exposed to increasing concentrations of the cluster (0.1 to 250 µM), by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. Hemolysis and morphological alterations of red blood cells, obtained from healthy donors, exposed to the cluster (10 to 200 µM) at 37 °C were analyzed. Furthermore, quenching of tryptophan residues of albumin was performed. Finally, plaque formation by rotavirus SA11 in MA104 cells treated with the cluster (100 to 300 µM) were analyzed. We found that all doses of the cluster showed similar cell viability, hemolysis, and morphology values, compared to control. Quenching of tryptophan residues of albumin suggests a protein-cluster complex formation. Finally, the cluster showed antiviral activity at 300 µM. These results indicate that the cluster [Mo6Cl14]2− could be intravenously administered in animals at therapeutic doses for further in vivo studies and might be studied as an antiviral agent.

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Antioxidative and cytoprotective response elicited by molybdenum nanoparticles in human cells

Cited by 49 publications

References 47 publications

Effect of Molybdenum Nanoparticles on Blood Cells, Liver Enzymes, and Sexual Hormones in Male Rats

Effect of Molybdenum Nanoparticles on Blood Cells, Liver Enzymes, and Sexual Hormones in Male Rats

MORPHOLOGICAL AND BIOCHEMICAL PARAMETERS IN Wistar RATS INFLUENCED BY MOLYBDENUM AND ITS OXIDE NANOPARTICLES

The [Mo6Cl14]2− Cluster is Biologically Secure and Has Anti-Rotavirus Activity In Vitro

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